Construct: ORF TRCN0000477050
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012253.1_s317c1
- Derived from:
- ccsbBroadEn_09792
- DNA Barcode:
- CAAAGAATCCAGTTCCGTGAGCGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ANO6 (196527)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477050
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 196527 | ANO6 | anoctamin 6 | NM_001025356.3 | 99.9% | 99.8% | 2509T>C |
| 2 | human | 196527 | ANO6 | anoctamin 6 | XM_005268706.5 | 98.2% | 97.4% | (many diffs) |
| 3 | human | 196527 | ANO6 | anoctamin 6 | NM_001142678.2 | 97.8% | 96.9% | (many diffs) |
| 4 | human | 196527 | ANO6 | anoctamin 6 | NM_001204803.2 | 97.7% | 97.6% | 70_132del;2572T>C |
| 5 | human | 196527 | ANO6 | anoctamin 6 | XM_005268707.4 | 96.3% | 92.9% | 0_1insATGAAAAAGATGAGCAGGA;51_52ins80;2410T>C |
| 6 | human | 196527 | ANO6 | anoctamin 6 | NM_001142679.2 | 90.6% | 87.3% | (many diffs) |
| 7 | mouse | 105722 | Ano6 | anoctamin 6 | NM_175344.4 | 83.2% | 90.1% | (many diffs) |
| 8 | mouse | 105722 | Ano6 | anoctamin 6 | XM_006520234.2 | 82.2% | 88.8% | (many diffs) |
| 9 | mouse | 105722 | Ano6 | anoctamin 6 | XM_006520233.3 | 82.2% | 87.6% | (many diffs) |
| 10 | mouse | 105722 | Ano6 | anoctamin 6 | XM_006520232.3 | 81.8% | 87.6% | (many diffs) |
| 11 | mouse | 105722 | Ano6 | anoctamin 6 | XM_006520231.3 | 81.7% | 87.7% | (many diffs) |
| 12 | mouse | 105722 | Ano6 | anoctamin 6 | NM_001253813.1 | 81.4% | 88.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2799
- ORF length:
- 2730
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaaaaagatg agcaggaatg ttttgctaca aatggaggag gaggaggacg 121 acgacgatgg ggatatcgtg ttggaaaacc ttggacagac aattgtcccc gatttgggat 181 cactggaaag tcagcatgat tttcgaaccc cggagtttga agaatttaat ggaaaacctg 241 actccctctt ttttaatgat ggccagcgaa gaattgactt tgttctagta tatgaggatg 301 aaagcagaaa agagaccaat aaaaagggta caaatgaaaa acaaaggagg aaaagacaag 361 catacgaatc taaccttatc tgtcatggcc tgcagttaga agcaacaaga tcagtattgg 421 atgacaagct tgtatttgta aaagtacacg caccatggga ggtgttatgt acgtatgctg 481 agataatgca catcaaattg cctctgaaac ccaatgatct gaaaaaccgg tcctcagcct 541 ttggtacact caactggttt accaaagtcc tcagtgtaga cgaaagcatc atcaagccag 601 agcaagagtt tttcactgcc ccatttgaga agaaccggat gaatgatttt tacatagttg 661 atagagatgc tttcttcaat ccagccacca gaagccgcat tgtttacttc atcctctctc 721 gggtcaagta tcaagtgata aacaatgtta gcaagtttgg gatcaacaga cttgtaaact 781 ctgggatcta caaggcagct ttcccactcc atgattgcaa attccgccgt cagtcagagg 841 atcccagctg ccctaatgaa cggtaccttc tgtacagaga atgggctcat cctcgaagca 901 tatacaaaaa gcagcccttg gatcttatca ggaaatacta tggagagaag attggaatct 961 actttgcttg gctgggctat tacactcaga tgcttctcct ggccgcagtt gtaggagtgg 1021 cttgctttct ctatggatat cttaatcaag ataactgtac atggagcaaa gaagtttgtc 1081 atcctgatat tggtggcaag atcataatgt gtcctcagtg tgataggctt tgtccattct 1141 ggaaactcaa tattacttgc gagtcctcaa agaaattgtg catcttcgac agttttggaa 1201 ccctggtctt tgcagtattt atgggagtat gggttacctt gtttttggag ttttggaagc 1261 gacgccaggc agaacttgag tatgaatggg atactgttga gttacagcag gaagaacaag 1321 cccgaccaga atacgaagca cgatgtactc acgtagtgat aaatgagatt actcaggaag 1381 aagaacgcat tccctttact gcctggggaa aatgtatacg gataaccctc tgtgccagtg 1441 ctgtcttttt ctggatccta ttgatcatcg cttcagttat tgggatcatt gtctataggc 1501 tctcggtgtt cattgtattt tctgcaaaac ttcccaagaa cattaatgga acagacccaa 1561 tccagaaata cctgactcca cagacagcca cgtccatcac ggcctccatc atcagcttta 1621 taattatcat gattctgaac accatatatg aaaaagtggc aattatgatt actaacttcg 1681 aactcccaag gacccagact gattatgaga acagcctcac catgaagatg ttcttattcc 1741 agtttgtcaa ctactactct tcatgcttct acatagcatt ctttaagggc aaatttgtag 1801 gctatccagg agacccagtt tattggttgg gaaaatacag aaatgaagag tgtgacccag 1861 gtggctgtct tcttgaactg acaactcagc tgacaataat catgggagga aaagcaatct 1921 ggaataacat acaagaagta ttattgccct ggatcatgaa tctaattggg cgatttcaca 1981 gagtttctgg atcagaaaag ataaccccac gatgggaaca ggactaccat ctgcagccta 2041 tgggcaaact gggattattt tatgaatatc ttgaaatgat tattcagttt gggttcgtca 2101 ccttatttgt ggcctctttt ccactggccc ctctgttggc tctcgtgaac aatatattgg 2161 aaataagagt ggacgcatgg aaactgacca cccagtttag acgcctggta ccagagaaag 2221 cccaagacat tggagcatgg cagcccatca tgcaaggaat agcaattctg gctgtggtga 2281 ccaatgccat gatcatagct ttcacgtcgg acatgatccc ccgcctagtg tactactggt 2341 ccttctccgt ccctccctac ggggaccaca cttcctacac catGGAAGGG TACATCAACA 2401 ACACTCTCTC CATCTTCAAA GTCGCAGACT TCAAAAACAA AAGCAAGGGA AACCCGTACT 2461 CTGACCTGGG TAACCATACC ACATGCAGGT ATCGTGATTT CCGATACCCA CCTGGACACC 2521 CCCAGGAGTA TAAACACAAC ATCTACTATT GGCATGTGAT TGCAGCCAAG CTGGCTCTTA 2581 TCATTGTCAT GGAGCACGTC ATCTACTCTG TGAAATTTTT CATTTCATAT GCAATTCCCG 2641 ATGTATCAAA ACGCACAAAG AGCAAGATCC AGAGAGAAAA ATACCTAACC CAAAAGCTTC 2701 TTCATGAGAA TCACCTCAAA GATATGACGA AAAATATGGG GGTGATAGCT GAGCGGATGA 2761 TAGAAGCAGT AGATAACAAT TTACGGCCAA AATCAGAATT GCCAACTTTC TTGTACAAAG 2821 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 2881 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 2941 ACGACAAAGA ATCCAGTTCC GTGAGCGGAC GCGTTAAGTC gacaatcaac ctctggatta 3001 caaaatttgt gaaagatt