Transcript: Human NM_001143821.3

Homo sapiens pleckstrin homology domain containing A5 (PLEKHA5), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-08-08
Taxon:
Homo sapiens (human)
Gene:
PLEKHA5 (54477)
Length:
6565
CDS:
68..3592

Additional Resources:

NCBI RefSeq record:
NM_001143821.3
NBCI Gene record:
PLEKHA5 (54477)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001143821.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000284379 ACACCCGAATCTTCGACAATA pLKO_005 2810 CDS 100% 13.200 18.480 N PLEKHA5 n/a
2 TRCN0000271423 CAACCATAGAGTGCTAATTAA pLKO_005 949 CDS 100% 15.000 10.500 N PLEKHA5 n/a
3 TRCN0000271366 CAGTTGGAACAGTGGATTAAA pLKO_005 1304 CDS 100% 15.000 10.500 N PLEKHA5 n/a
4 TRCN0000033846 CGGCCAATAAGTATGATAAAT pLKO.1 416 CDS 100% 15.000 10.500 N PLEKHA5 n/a
5 TRCN0000271365 CCCGCTACCCTGAAGGTTATA pLKO_005 1422 CDS 100% 13.200 9.240 N PLEKHA5 n/a
6 TRCN0000033844 GCCGATTATGTGAACAAGATA pLKO.1 2160 CDS 100% 5.625 3.938 N PLEKHA5 n/a
7 TRCN0000033848 GCAACAGAAATTGTTCAACTA pLKO.1 3167 CDS 100% 4.950 3.465 N PLEKHA5 n/a
8 TRCN0000033845 GCACCAACTAAAGAAACCAAT pLKO.1 920 CDS 100% 4.950 3.465 N PLEKHA5 n/a
9 TRCN0000033847 GCCTGACTTTACAGTCTGTTA pLKO.1 1866 CDS 100% 4.950 3.465 N PLEKHA5 n/a
10 TRCN0000256745 CAGCCTGGCCAACATGGTAAA pLKO_005 6065 3UTR 100% 10.800 5.400 Y SMIM11A n/a
11 TRCN0000007228 CACCTGTAATCCCAGCACTTT pLKO.1 6298 3UTR 100% 4.950 2.475 Y CFLAR n/a
12 TRCN0000166635 CACCTGTAATCCCAGCACTTT pLKO.1 6298 3UTR 100% 4.950 2.475 Y C19orf31 n/a
13 TRCN0000021429 CACACCTGTAATCCCAGCATT pLKO.1 6296 3UTR 100% 4.950 2.475 Y ERN2 n/a
14 TRCN0000138998 CACACCTGTAATCCCAGCATT pLKO.1 6296 3UTR 100% 4.950 2.475 Y P3H4 n/a
15 TRCN0000344020 CACACCTGTAATCCCAGCATT pLKO_005 6296 3UTR 100% 4.950 2.475 Y P3H4 n/a
16 TRCN0000138772 GCAGGAGAATCGCTTGAACTT pLKO.1 6455 3UTR 100% 4.950 2.475 Y DCAF11 n/a
17 TRCN0000164834 CAAACTCCTGAGCTCAAGCAA pLKO.1 5869 3UTR 100% 3.000 1.500 Y LINC00336 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001143821.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12044 pDONR223 100% 74.4% 74.4% None 1_726del;1845_2018del n/a
2 ccsbBroad304_12044 pLX_304 0% 74.4% 74.4% V5 1_726del;1845_2018del n/a
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