Construct: ORF ccsbBroadEn_12044
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008225.1_s300c1, BRDN0000457262
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PLEKHA5 (54477)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54477 | PLEKHA5 | pleckstrin homology domain ... | XM_017019498.1 | 79.9% | 79.9% | 1_402del;1522_1776del |
| 2 | human | 54477 | PLEKHA5 | pleckstrin homology domain ... | NM_001256787.1 | 79.5% | 79.5% | 1_402del;1522_1602del;2158_2346del |
| 3 | human | 54477 | PLEKHA5 | pleckstrin homology domain ... | NM_019012.6 | 78.3% | 78.3% | 1_726del |
| 4 | human | 54477 | PLEKHA5 | pleckstrin homology domain ... | XM_017019497.1 | 74.8% | 74.8% | 1_402del;1522_1812del;2368_2556del |
| 5 | human | 54477 | PLEKHA5 | pleckstrin homology domain ... | NM_001143821.3 | 74.4% | 74.4% | 1_726del;1845_2018del |
| 6 | human | 54477 | PLEKHA5 | pleckstrin homology domain ... | XM_011520717.1 | 74.4% | 74.4% | 1_726del;1845_2018del |
| 7 | human | 54477 | PLEKHA5 | pleckstrin homology domain ... | XM_005253400.1 | 74.1% | 74.1% | 1_726del;2401_2589del |
| 8 | human | 54477 | PLEKHA5 | pleckstrin homology domain ... | XM_006719091.1 | 73% | 73% | 1_726del;1845_1898del;2455_2643del |
| 9 | human | 54477 | PLEKHA5 | pleckstrin homology domain ... | XM_011520716.1 | 72% | 72% | 1_726del;1846_2136del |
| 10 | human | 54477 | PLEKHA5 | pleckstrin homology domain ... | XM_006719093.1 | 71.7% | 71.7% | 1_726del;1760_1761ins84;2317_2505del |
| 11 | human | 54477 | PLEKHA5 | pleckstrin homology domain ... | XM_017019496.1 | 71.6% | 71.6% | 1_726del;1846_2136del;2627_2628insAGGTACTACAGAAAT |
| 12 | human | 54477 | PLEKHA5 | pleckstrin homology domain ... | XM_011520715.1 | 70.6% | 70.6% | 1_726del;1845_2018del;2575_2763del |
| 13 | human | 54477 | PLEKHA5 | pleckstrin homology domain ... | XM_011520714.1 | 69.1% | 69.1% | 1_726del;1846_2100del;2656_2844del |
| 14 | human | 54477 | PLEKHA5 | pleckstrin homology domain ... | XM_006719096.1 | 68.4% | 68.4% | 1_726del;1846_2136del;2692_2880del |
| 15 | human | 54477 | PLEKHA5 | pleckstrin homology domain ... | NM_001256470.2 | 68.1% | 68.1% | (many diffs) |
| 16 | human | 54477 | PLEKHA5 | pleckstrin homology domain ... | XM_017019495.1 | 68.1% | 68.1% | (many diffs) |
| 17 | human | 54477 | PLEKHA5 | pleckstrin homology domain ... | XM_017019499.1 | 50.4% | 49.4% | (many diffs) |
| 18 | human | 54477 | PLEKHA5 | pleckstrin homology domain ... | XR_001748772.1 | 39.7% | (many diffs) | |
| 19 | human | 54477 | PLEKHA5 | pleckstrin homology domain ... | XM_017019500.1 | 37.6% | 34.8% | (many diffs) |
| 20 | human | 54477 | PLEKHA5 | pleckstrin homology domain ... | XM_017019501.1 | 34.2% | 33.6% | (many diffs) |
| 21 | mouse | 109135 | Plekha5 | pleckstrin homology domain ... | XM_006506892.2 | 35.3% | 36.8% | (many diffs) |
| 22 | mouse | 109135 | Plekha5 | pleckstrin homology domain ... | XR_378094.2 | 35.1% | (many diffs) | |
| 23 | mouse | 109135 | Plekha5 | pleckstrin homology domain ... | XR_001785083.1 | 35.1% | (many diffs) | |
| 24 | mouse | 109135 | Plekha5 | pleckstrin homology domain ... | XR_378093.2 | 35.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2691
- ORF length:
- 2622
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAGAAAG 61 TTGGCACCAT GCGGACCTAT TATTTCTGCA CTGATACAGG AAAGGAAATG GAGTTGTGGA 121 TGAAAGCCAT GTTAGATGCT GCCCTAGTAC AGACAGAACC TGTGAAAAGA GTGGACAAGA 181 TTACATCTGA AAATGCACCA ACTAAAGAAA CCAATAACAT TCCCAACCAT AGAGTGCTAA 241 TTAAACCAGA GATCCAAAAC AATCAAAAAA ACAAGGAAAT GAGCAAAATT GAAGAAAAAA 301 AGGCATTAGA AGCTGAAAAA TATGGATTTC AGAAGGATGG TCAAGATAGA CCCTTAACAA 361 AAATTAATAG TGTAAAGCTG AATTCTCTGC CATCTGAATA TGAGAGTGGG TCAGCATGCC 421 CTGCTCAGAC TGTGCACTAC AGACCAATCA ACTTGAGCAG TTCAGAGAAC AAAATAGTCA 481 ATGTTAGCCT GGCAGATCTT AGAGGTGGAA ATCGCCCCAA TACAGGGCCC TTATACACAG 541 AGGCCGATCG AGTCATACAG AGAACAAATT CAATGCAGCA GTTGGAACAG TGGATTAAAA 601 TCCAGAAGGG GAGGGGTCAT GAAGAAGAAA CCAGGGGAGT AATTTCTTAC CAAACATTAC 661 CAAGAAATAT GCCAAGTCAC AGAGCCCAGA TTATGGCCCG CTACCCTGAA GGTTATAGAA 721 CACTCCCAAG AAACAGCAAG ACAAGGCCTG AAAGTATCTG CAGTGTAACC CCTTCCACTC 781 ATGACAAGAC ATTAGGACCC GGAGCGGAGG AGAAACGGAG GTCCATGAGA GATGACACAA 841 TGTGGCAGCT CTACGAATGG CAGCAGCGTC AGTTTTATAA CAAACAGAGC ACCCTCCCTC 901 GACACAGTAC TTTGAGTAGT CCCAAAACCA TGGTAAATAT TTCTGACCAG ACAATGCACT 961 CTATTCCCAC ATCACCTTCC CACGGGTCAA TAGCTGCTTA TCAGGGATAC TCCCCTCAAC 1021 GAACTTACAG ATCGGAAGTG TCTTCACCAA TTCAGAGAGG AGATGTGACA ATAGACCGCA 1081 GACACAGGGC CCATCACCCT AAGCATGTCT ATGTGCCTGA CAGAAGGTCA GTGCCAGCTG 1141 GCCTGACTTT ACAGTCTGTT AGTCCCCAGA GCCTCCAAGG GAAAACGCTG TCACAAGATG 1201 AAGGTAGAGG CACATTATAC AAATACAGAC CTGAAGAAGT AGATATTGAT GCCAAGTTAA 1261 GCCGATTATG TGAACAAGAT AAAGTGGTGC ATGCTCTGGA AGAGAAACTT CAGCAACTCC 1321 ACAAGGAGAA ATACACGCTT GAGCAAGCTT TGCTATCAGC CAGCCAAGAG ATAGAAATGC 1381 ATGCAGATAA CCCAGCAGCC ATTCAGACAG TGGTGTTACA AAGGGATGAT TTACAAAATG 1441 GACTGCTTAG TACGTGTCGA GAACTTTCTC GAGCCACTGC CGAATTGGAA CGAGCATGGA 1501 GAGAATATGA TAAGTTAGAA TACGATGTAA CTGTTACCAG GAACCAGATG CAAGAGCAGC 1561 TGGATCACCT TGGTGAAGTT CAGACGGAAT CAGCAGGAAT TCAGCGTGCA CAGATTCAGA 1621 AAGAACTTTG GCGAATTCAG GATGTCATGG AAGGGCTGAG TAAACATAAG CAGCAAAGAG 1681 GTACTACAGA AATAGGTATG ATAGGATCAA AGCCTTTCTC AACAGTTAAG TACAAAAATG 1741 AGGGTCCAGA TTATAGACTC TACAAGAGTG AACCAGAGTT AACAACAGTG GCAGAAGTTG 1801 ATGAATCTAA TGGAGAAGAA AAATCAGAAC CTGTTTCAGA GATAGAAACT TCAGTTGTTA 1861 AAGGTTCCCA CTTTCCTGTT GGAGTAGTCC CTCCAAGAGC AAAATCACCA ACACCCGAAT 1921 CTTCGACAAT AGCTTCCTAT GTAACCTTGA GGAAAACTAA GAAGATGATG GATCTAAGAA 1981 CGGAAAGACC AAGAAGTGCA GTGGAACAGC TCTGTTTGGC TGAAAGTACT CGACCAAGGA 2041 TGACTGTGGA AGAGCAAATG GAAAGAATAA GAAGACATCA ACAAGCGTGC CTGAGGGAGA 2101 AGAAAAAAGG GTTAAATGTT ATCGGTGCTT CAGACCAGTC ACCCTTACAA AGCCCTTCAA 2161 ATTTAAGGGA TAATCCATTT AGGACTACTC AGACTCGAAG GAGGGATGAT AAGGAACTGG 2221 ACACTGCCAT TAGAGAAAAT GATGTAAAGC CAGACCATGA AACTCCTGCA ACAGAAATTG 2281 TTCAACTAAA AGAAACCGAA CCCCAAAATG TGGACTTCAG CAAAGAGTTA AAAAAAACTG 2341 AAAACATTTC ATATGAAATG CTTTTTGAAC CTGAGCCAAA TGGAGTAAAT TCTGTGGAAA 2401 TGATGGATAA AGAAAGAAAC AAAGACAAAA TGCCTGAGGA TGTTACATTC AGCCCTCAAG 2461 ATGAAACACA GACCGCAAAT CATAAACCAG AAGAGCATCC TGAAGAAAAT ACAAAGAACA 2521 GTGTTGACGA ACAGGAAGAA ACTGTTATTT CTTACGAATC AACTCCTGAG GTTTCTAGAG 2581 GAAATCAAAC AATGGCAGTG AAAAGTCTGT CCCCATCTCC TGAGTCCTCG GCATCGCCAG 2641 TTCCATCCAC TCAGCCGCAG CTCACAGAAG GATCACATTT CATGTGTGTG TTGCCAACTT 2701 TCTTGTACAA AGTTGGcatt ataagaaagc attgcttatc aatttgttgc aacgaac