Construct: ORF TRCN0000481400
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015567.1_s317c1
- Derived from:
- ccsbBroadEn_12147
- DNA Barcode:
- ACACTTGTGTCTAGTGCGGATGTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CRYBG2 (55057)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481400
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55057 | CRYBG2 | crystallin beta-gamma domai... | NM_001039775.3 | 37% | 37% | 1_3135del |
| 2 | human | 55057 | CRYBG2 | crystallin beta-gamma domai... | XM_005245918.2 | 37% | 37% | 1_3135del |
| 3 | human | 55057 | CRYBG2 | crystallin beta-gamma domai... | XM_011541672.1 | 36.3% | 36.3% | 1_3135del;3470_3471ins36 |
| 4 | human | 55057 | CRYBG2 | crystallin beta-gamma domai... | XM_011541673.2 | 35.8% | 35.8% | 1_3306del |
| 5 | human | 55057 | CRYBG2 | crystallin beta-gamma domai... | XR_946681.1 | 33.2% | 1_3271del;4712_5039del;5448_5558del | |
| 6 | human | 55057 | CRYBG2 | crystallin beta-gamma domai... | XR_001737260.1 | 33.1% | 1_3271del;4205_4206ins113;5007_5117del | |
| 7 | mouse | 230806 | Crybg2 | crystallin beta-gamma domai... | NM_001162970.1 | 34.6% | 35.4% | (many diffs) |
| 8 | mouse | 230806 | Crybg2 | crystallin beta-gamma domai... | XM_006538750.2 | 34.6% | 35.4% | (many diffs) |
| 9 | mouse | 230806 | Crybg2 | crystallin beta-gamma domai... | XM_006538751.3 | 34.6% | 35.4% | (many diffs) |
| 10 | mouse | 230806 | Crybg2 | crystallin beta-gamma domai... | XM_006538748.3 | 28.7% | 29.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1917
- ORF length:
- 1848
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaactcaga accccaggga caaagtggag tccccaaggc atcggctccc 121 taaggagggt tgtctgggac tacagcaccc cggaaatcag cctcttctct gaggagggcc 181 tcaaggggga gcaagtgaag ctaacagagg ccttgaagaa ttcccagggt ctggagaagc 241 ccctgcaggt ggcatctgcc accgtctctg caggactgtg gctactgtac cccaaaccat 301 tattcgaaga cactccctac atcctggaac ctggagagta ccccacctca gaggcctggg 361 gcacatcgga ccccagcgtg ggctccctga agcccatgag attgggctgc ccaagtgtgg 421 agaagccagg ggagcccagg gctgtggtgt atgaggcccc aggctttcag ggccgcagct 481 gggaagtgag ccgagacatc tacaaccttc agcagccaga ggacagccag agcccccacc 541 tggcctctgt ggggtccctg agagttctcg gaggctgctg ggtgggctac gagaaggagg 601 gcttccgggg ccaccagtat ctgctggagg agggggaata cccagactgg tcacactggg 661 gaggctatga cgagttgctg acctccctcc gggtcatccg gacggacttc ggggacccgg 721 ccgtcgtgct atttgaggcc atggacttcg aggggcacgg cgtggaggtg agcaaggcat 781 tgccggatgt ggagctggtg caacacggcc ccagcacaca ggccatccac gtgctcagcg 841 gcgtgtgggt ggcctaccag gaggtgggct tctccgggga acagtacgtg ctggagaagg 901 gcgtgtatcg taactgcgag gactggggcg ctggcaacag caccctcgcc tcgctgcagc 961 cggtcctaca ggtcggggag cacgatctgc acttcgtctc aaagattcag cttttctccc 1021 gccccgactt tctgggcgac cacttctctt tcgaagatga ccaggccgct ctgcccgcct 1081 ccttccgacc tcagtcctgc cgggtccacg gcggcagctg gatcctgttt gatgagacga 1141 acttcgaggg tgaccagcac attctctctg agggcgagtt ccccactctc acggccatgg 1201 gctgcctcgc ctccacagtc ctgggctctc tccagaaggt atccctgcac ttttcagagc 1261 cttccatttt cctgtatgga ctcgagtgct tcgaggggaa ggagatcgag ctcagcaggg 1321 aggtgcggag cctgcaagcc gagggcttca acaaccatgt gctgtctgtg cggatcaagg 1381 ggggcatttg ggtgctatgt gaacacagtg acttccgggg ccgccagtgg ctggtgggaa 1441 gctgcgagat caccaactgg ctgacctaca gcggcaccca gagggtgggc tccctctacc 1501 ccatcaagca gcgccgggtt tatttccgcc TCTGGAATGC AGCACTGGGG GGATTCCTGG 1561 CAGTGCCGGA CCATGTGGAG GACATGAAAG CAGGCCGTGT GGTGGTCGCC GACCCCCAAG 1621 CTGGAGGTAG CTGCATCTGG TACTACGAGG ATGGGCTGCT GAAGAACCAG ATGGCCCCCA 1681 CCATGAGCCT ACAGGTGATT GGACCCCCTA GCCCAGGCTC CAAGGTGGTG CTGTGGGCCG 1741 AGAGCCGCCT GCCGCGCCAG ACGTGGAGCA TCAGTGAATC GGGCCACATC TGCAGCCAGA 1801 TGTTCGAAGG CCAGATCCTG GACGTGAAGG GAGGCCGGGG CTACGACCGG GACCACGTGG 1861 TGCTATGGGA GCCGGATGAG GACAGGGCAT CCCAGATCTG GACTATCCAC GTGCTTTTGC 1921 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1981 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 2041 ATATATCTTG TGGAAAGGAC GAACACTTGT GTCTAGTGCG GATGTAACGC GTTAAGTCga 2101 caatcaacct ctggattaca aaatttgtga aagatt