Construct: ORF TRCN0000467211

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015282.1_s317c1
Derived from:
ccsbBroadEn_10680
DNA Barcode:
TCGACAATGATCTCATCATGGCAT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
APBB2 (323)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000467211
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 323 APBB2 amyloid beta precursor prot... NM_001166051.2 63.3% 63.3% 0_1ins360;84_86delAGT
2 human 323 APBB2 amyloid beta precursor prot... NM_001166052.2 63.3% 63.3% 0_1ins360;84_86delAGT
3 human 323 APBB2 amyloid beta precursor prot... NM_001166053.1 63.3% 63.3% 0_1ins360;84_86delAGT
4 human 323 APBB2 amyloid beta precursor prot... NM_001166054.1 63.3% 63.3% 0_1ins360;84_86delAGT
5 human 323 APBB2 amyloid beta precursor prot... XM_017008158.2 45.6% 45.6% 1_1173del;1617_1619delAGT
6 human 323 APBB2 amyloid beta precursor prot... XM_017008156.2 45.5% 45.5% 1_1173del;1415_1420delGAGAGA;1623_1625delAGT
7 human 323 APBB2 amyloid beta precursor prot... XM_017008157.2 45.5% 45.5% 1_1173del;1415_1420delGAGAGA;1623_1625delAGT
8 human 323 APBB2 amyloid beta precursor prot... NM_173075.5 44.7% 44.7% 1_1221del
9 human 323 APBB2 amyloid beta precursor prot... NM_001330656.2 44.6% 44.6% 1_1221del;1665_1667delAGT
10 human 323 APBB2 amyloid beta precursor prot... NM_001330658.2 44.6% 44.6% 1_1221del;1665_1667delAGT
11 human 323 APBB2 amyloid beta precursor prot... XM_011513687.3 44.5% 44.5% 1_1224del;1668_1670delAGT
12 human 323 APBB2 amyloid beta precursor prot... XM_017008154.2 44.5% 44.5% 1_1224del;1668_1670delAGT
13 human 323 APBB2 amyloid beta precursor prot... XM_006714012.4 44.5% 44.5% 1_1221del;1463_1468delGAGAGA;1671_1673delAGT
14 human 323 APBB2 amyloid beta precursor prot... XM_017008152.2 44.5% 44.5% 1_1221del;1463_1468delGAGAGA;1671_1673delAGT
15 human 323 APBB2 amyloid beta precursor prot... XM_006714011.4 44.4% 44.4% 1_1224del;1466_1471delGAGAGA;1674_1676delAGT
16 human 323 APBB2 amyloid beta precursor prot... XM_017008151.2 44.4% 44.4% 1_1224del;1466_1471delGAGAGA;1674_1676delAGT
17 human 323 APBB2 amyloid beta precursor prot... XM_017008149.2 44.2% 44.2% 1_1233del;1475_1480delGAGAGA;1683_1685delAGT
18 human 323 APBB2 amyloid beta precursor prot... XM_017008146.2 44.2% 44.2% 1_1236del;1478_1483delGAGAGA;1686_1688delAGT
19 human 323 APBB2 amyloid beta precursor prot... XM_017008147.2 44.2% 44.2% 1_1236del;1478_1483delGAGAGA;1686_1688delAGT
20 human 323 APBB2 amyloid beta precursor prot... XM_017008148.2 44.2% 44.2% 1_1236del;1478_1483delGAGAGA;1686_1688delAGT
21 human 323 APBB2 amyloid beta precursor prot... NM_001166050.2 43.4% 43.4% 1_1284del;1728_1730delAGT
22 human 323 APBB2 amyloid beta precursor prot... NM_004307.2 43.3% 43.3% 1_1287del;1731_1733delAGT
23 human 323 APBB2 amyloid beta precursor prot... XM_017008145.2 43.3% 43.3% 1_1287del;1731_1733delAGT
24 human 323 APBB2 amyloid beta precursor prot... XM_006714010.4 43.2% 43.2% 1_1284del;1526_1531delGAGAGA;1734_1736delAGT
25 human 323 APBB2 amyloid beta precursor prot... XM_017008143.2 43.2% 43.2% 1_1284del;1526_1531delGAGAGA;1734_1736delAGT
26 human 323 APBB2 amyloid beta precursor prot... XM_017008144.2 43.2% 43.2% 1_1284del;1526_1531delGAGAGA;1734_1736delAGT
27 human 323 APBB2 amyloid beta precursor prot... XM_006714005.4 43.2% 43.2% 1_1287del;1529_1534delGAGAGA;1737_1739delAGT
28 human 323 APBB2 amyloid beta precursor prot... XM_006714007.4 43.2% 43.2% 1_1287del;1529_1534delGAGAGA;1737_1739delAGT
29 human 323 APBB2 amyloid beta precursor prot... XM_006714008.4 43.2% 43.2% 1_1287del;1529_1534delGAGAGA;1737_1739delAGT
30 human 323 APBB2 amyloid beta precursor prot... XM_006714009.4 43.2% 43.2% 1_1287del;1529_1534delGAGAGA;1737_1739delAGT
31 human 323 APBB2 amyloid beta precursor prot... XM_011513686.3 43.2% 43.2% 1_1287del;1529_1534delGAGAGA;1737_1739delAGT
32 mouse 11787 Apbb2 amyloid beta (A4) precursor... XM_017320622.1 59.9% 65% (many diffs)
33 mouse 11787 Apbb2 amyloid beta (A4) precursor... NM_001201416.1 56.3% 60.3% (many diffs)
34 mouse 11787 Apbb2 amyloid beta (A4) precursor... XM_006503696.3 56.3% 60.3% (many diffs)
35 mouse 11787 Apbb2 amyloid beta (A4) precursor... XM_006503697.3 56.3% 60.3% (many diffs)
36 mouse 11787 Apbb2 amyloid beta (A4) precursor... NM_001310626.1 39.7% 43% (many diffs)
37 mouse 11787 Apbb2 amyloid beta (A4) precursor... NM_001201415.1 39.6% 43% (many diffs)
38 mouse 11787 Apbb2 amyloid beta (A4) precursor... NM_001201414.1 39.5% 42.9% (many diffs)
39 mouse 11787 Apbb2 amyloid beta (A4) precursor... XM_006503694.3 39.5% 42.9% (many diffs)
40 mouse 11787 Apbb2 amyloid beta (A4) precursor... XM_006503693.3 39.4% 42.8% (many diffs)
41 mouse 11787 Apbb2 amyloid beta (A4) precursor... NM_001201413.1 38.5% 41.8% (many diffs)
42 mouse 11787 Apbb2 amyloid beta (A4) precursor... XM_006503692.3 38.5% 41.8% (many diffs)
43 mouse 11787 Apbb2 amyloid beta (A4) precursor... XM_017320620.1 38.4% 41.7% (many diffs)
44 mouse 11787 Apbb2 amyloid beta (A4) precursor... XM_017320621.1 38.4% 41.7% (many diffs)
45 mouse 11787 Apbb2 amyloid beta (A4) precursor... NM_009686.2 38.4% 41.7% (many diffs)
46 mouse 11787 Apbb2 amyloid beta (A4) precursor... XM_011240693.2 38.4% 41.7% (many diffs)
47 mouse 11787 Apbb2 amyloid beta (A4) precursor... XM_006503688.3 38.3% 41.6% (many diffs)
48 mouse 11787 Apbb2 amyloid beta (A4) precursor... XM_006503690.3 38.3% 41.6% (many diffs)
49 mouse 11787 Apbb2 amyloid beta (A4) precursor... XM_006503691.3 38.3% 41.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1053
ORF length:
987
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc agaagaggac ctcgcccccg gtaaaagtag tgttgcggtc aacaactgca
121tcaggcaact ttcctactgc aaaaatgaca tccgagacac agtcgggatt tggggagagg
181ggaaagacat gtacctgatc ctggagaatg acatgctcag cctggtggac cccatggacc
241gcagcgtgct gcactcgcag cccatcgtca gcatccgcgt gtggggcgtg ggccgcgaca
301atggccggga ttttgcttat gtagcaagag ataaagatac aagaattttg aaatgtcatg
361tatttcgatg tgacacacca gcaaaagcca ttgccacaag tctccacgag atctgctcca
421agattatggc tgaacggaag aatgccaaag cgctggcctg cagctcctta caggaaaggg
481ccaatgtgaa cctcgatgtc cctttgcaag attttccaac accaaagact gagctggtcc
541agaagttcca cgtgcagtac ttgggcatgt tacctgtaga caaaccagtc ggaatggata
601ttttgaacag tgccatagaa aatcttatga cctcatccaa caaggaggac tggctgtcag
661tgaacatgaa cgtggctgat gccactgtga ctgtcatcag tgaaaagaat gaagaggaag
721tcttagtgga atgtcgtgtg cgattcctgt ccTTCATGGG TGTTGGGAAG GACGTCCACA
781CATTTGCCTT CATCATGGAC ACGGGGAACC AGCGCTTTGA GTGCCACGTT TTCTGGTGCG
841AGCCTAATGC TGGTAACGTG TCTGAGGCGG TGCAGGCCGC CTGCATGTTA CGATATCAGA
901AGTGCTTGGT AGCCAGGCCG CCTTCTCAGA AAGTTCGACC ACCTCCACCG CCAGCAGATT
961CAGTAACCAG AAGAGTCACA ACCAATGTAA AACGAGGGGT CTTATCCCTC ATTGACACTT
1021TGAAACAGAA ACGCCCTGTC ACCGAAATGC CATACCCAAC TTTCTTGTAC AAAGTGGTTG
1081ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA
1141GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATC
1201GACAATGATC TCATCATGGC ATACGCGTTA AGTCgacaat caacctctgg attacaaaat
1261ttgtgaaaga tt

Download FASTA (ORF) (Full)