Transcript: Mouse NM_001170742.1

Mus musculus WD repeat domain 59 (Wdr59), transcript variant 3, mRNA.

Source:
NCBI, updated 2017-06-26
Taxon:
Mus musculus (mouse)
Gene:
Wdr59 (319481)
Length:
5111
CDS:
127..3051

Additional Resources:

NCBI RefSeq record:
NM_001170742.1
NBCI Gene record:
Wdr59 (319481)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001170742.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000157812 CCGGAATGTCAATGTGGAGAT pLKO.1 1332 CDS 100% 4.050 5.670 N WDR59 n/a
2 TRCN0000217337 GAACGAGACCAGCATGATAAA pLKO.1 2644 CDS 100% 13.200 9.240 N Wdr59 n/a
3 TRCN0000152608 GCCTCGGAAATACCTCAATAT pLKO.1 795 CDS 100% 13.200 9.240 N WDR59 n/a
4 TRCN0000178460 GAGAGACAGAACACATATCTT pLKO.1 2552 CDS 100% 5.625 3.938 N Wdr59 n/a
5 TRCN0000181360 CCAAGGACTATCAACTGGTTA pLKO.1 1016 CDS 100% 4.950 3.465 N Wdr59 n/a
6 TRCN0000182517 CCTCACAAAGGGATCGAGTTT pLKO.1 2800 CDS 100% 4.950 3.465 N Wdr59 n/a
7 TRCN0000182035 CGTGATTTAGGATGGAGAGTA pLKO.1 3156 3UTR 100% 4.950 3.465 N Wdr59 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001170742.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12603 pDONR223 100% 51.6% 55.9% None (many diffs) n/a
2 ccsbBroad304_12603 pLX_304 0% 51.6% 55.9% V5 (many diffs) n/a
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