Transcript: Human NM_001172575.1

Homo sapiens microcephalin 1 (MCPH1), transcript variant 3, mRNA.

Source:
NCBI, updated 2019-09-21
Taxon:
Homo sapiens (human)
Gene:
MCPH1 (79648)
Length:
3629
CDS:
77..1765

Additional Resources:

NCBI RefSeq record:
NM_001172575.1
NBCI Gene record:
MCPH1 (79648)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001172575.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000423827 ACACTTATCAAGCCTAATTAA pLKO_005 370 CDS 100% 15.000 10.500 N MCPH1 n/a
2 TRCN0000426642 GAAGTTGGAAGGATCCATTAA pLKO_005 679 CDS 100% 13.200 9.240 N MCPH1 n/a
3 TRCN0000083786 GAGACGTTTGAAGAGAAGTAT pLKO.1 902 CDS 100% 5.625 3.938 N MCPH1 n/a
4 TRCN0000083787 CGTAAATGTATGCAGCCCAAA pLKO.1 398 CDS 100% 4.050 2.835 N MCPH1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001172575.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12581 pDONR223 100% 76.3% 76% None (many diffs) n/a
2 ccsbBroad304_12581 pLX_304 0% 76.3% 76% V5 (many diffs) n/a
3 TRCN0000468585 TCAACCTCTCCTATCTTCTGCACC pLX_317 20.5% 76.3% 76% V5 (many diffs) n/a
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