Construct: ORF TRCN0000468585
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015697.1_s317c1
- Derived from:
- ccsbBroadEn_12581
- DNA Barcode:
- TCAACCTCTCCTATCTTCTGCACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MCPH1 (79648)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468585
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79648 | MCPH1 | microcephalin 1 | NM_001322045.1 | 89.2% | 89% | (many diffs) |
| 2 | human | 79648 | MCPH1 | microcephalin 1 | NM_001322043.1 | 84.5% | 84.3% | (many diffs) |
| 3 | human | 79648 | MCPH1 | microcephalin 1 | NM_001172574.1 | 84.2% | 84% | (many diffs) |
| 4 | human | 79648 | MCPH1 | microcephalin 1 | NM_001172575.1 | 76.3% | 76% | (many diffs) |
| 5 | human | 79648 | MCPH1 | microcephalin 1 | NM_001363980.2 | 69.1% | 68.8% | (many diffs) |
| 6 | human | 79648 | MCPH1 | microcephalin 1 | XM_011534759.3 | 68.9% | 68.6% | (many diffs) |
| 7 | human | 79648 | MCPH1 | microcephalin 1 | XM_011534758.3 | 68.2% | 67.9% | (many diffs) |
| 8 | human | 79648 | MCPH1 | microcephalin 1 | XM_017013833.2 | 67.1% | 66.8% | (many diffs) |
| 9 | human | 79648 | MCPH1 | microcephalin 1 | XM_011534757.3 | 67% | 66.7% | (many diffs) |
| 10 | human | 79648 | MCPH1 | microcephalin 1 | XM_011534756.3 | 66.1% | 65.8% | (many diffs) |
| 11 | human | 79648 | MCPH1 | microcephalin 1 | XM_017013832.2 | 65.9% | 65.6% | (many diffs) |
| 12 | human | 79648 | MCPH1 | microcephalin 1 | XM_017013831.2 | 63.8% | 63.5% | (many diffs) |
| 13 | human | 79648 | MCPH1 | microcephalin 1 | NM_001363979.1 | 61.8% | 61.5% | (many diffs) |
| 14 | human | 79648 | MCPH1 | microcephalin 1 | NM_024596.5 | 61.4% | 61.1% | (many diffs) |
| 15 | human | 79648 | MCPH1 | microcephalin 1 | XM_011534755.3 | 58.8% | 58.6% | (many diffs) |
| 16 | human | 79648 | MCPH1 | microcephalin 1 | NM_001322042.2 | 58.6% | 58.4% | (many diffs) |
| 17 | human | 79648 | MCPH1 | microcephalin 1 | XM_017013829.2 | 58.4% | 58.2% | (many diffs) |
| 18 | human | 79648 | MCPH1 | microcephalin 1 | XM_011534760.2 | 55.8% | 55.6% | (many diffs) |
| 19 | human | 79648 | MCPH1 | microcephalin 1 | NR_136159.1 | 40.8% | (many diffs) | |
| 20 | human | 79648 | MCPH1 | microcephalin 1 | XR_001745596.2 | 29% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1611
- ORF length:
- 1545
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa tgaacactta tcaagcctaa ttaaaaaaaa acgtaaatgt atgcagccca 121 aggattttaa ttttaaaaca ccagaaaatg ataagagatt tcagaagaaa tttgagaaaa 181 tggctaaaga gctacaaagg caaaaaacaa atctagatga tgatgtacct attctcttat 241 ttgaatctaa tggttcatta atatatactc ccacaattga aattaatagt agtcaccaca 301 gcgcaatgga gaagagatta caagagatga aggagaaaag ggaaaatctt tcccccacct 361 cttcccaaat gattcagcag tctcatgata atccaagtaa ctctctgtgt gaagcacctt 421 tgaacatttc acgtgatact ttgtgttcag atgaatactt tgctggtggc ttacactcat 481 cttttgatga tctttgtgga aactcaggat gtggaaatca ggaaaggaag ttggaaggat 541 ccattaatga cattaaaagt gatgtgtgta tttcttcact tgtattgaaa gcaaataata 601 ttcattcatc accatctttc actcacctcg ataaatcaag tcctcagaaa tttctgagta 661 atctttcaaa ggaagaaata aacttgcaaa gaaatattgc aggtaaagta gtcacccctc 721 accaaaagca ggctgcaggt atgtctcagg agacgtttga agagaagtat cgtttgtctc 781 ctaccttatc ttcaacaaaa ggccaccttt tgatacattc aagacccagg agttcctcag 841 taaagagaaa aagagtatca catggctccc attcacctcc gaaggaaaaa tgcaagagaa 901 agaggagcac caggagatct atcatgccga ggctgcagct gtgcaggtcg gaaggcaggc 961 tgcagcacgt ggcgggacct gccctggagg ctcttagctg tggggagtct tcatatgatg 1021 actatttttc acctgataat cttaaggaaa ggtattcaga gaatcttcct cctgaatctc 1081 agctgccatc aagccctgct cagttgagct gcagaagtct ttctaagaag gagagaacaa 1141 gcatatttga aatgtctgat ttttcctgcg ttggcaaaaa aaccagaaca gttgacatta 1201 ccaatttcac agcaaaaacc atctccagtc ctcggaaaac tggaaatggt gaaggccgtg 1261 caacttcgag ttgcgtgact tctgcccctg aagaagcccT AAGGTGTTGT AGACAGGCTG 1321 GGAAAGAAGA CGCATGCCCA GAGGGAAATG GCTTTTCTTA CACCATTGAG GACCCTGCTC 1381 TTCCAAAAGG ACATGATGAT GATTTAACTC CTTTGGAAGG AAGCCTTGAA GAAATGAAAG 1441 AAGCGGTTGG TCTGAAAAGC ACACAGAACA AAGGTACCAC TTCCAAAATA TCAAACTCCT 1501 CTGAAGGCGA AGCCCAGAGT GAACATGAGC CATGTTTTAT AGTTGACTGT AACATGGAGA 1561 CGTCTACAGA AGAGAAGGAA AACTTACCCG GAGGATACAG TGGAAGTATG TGCCCAACTT 1621 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1681 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1741 CTTGTGGAAA GGACGATCAA CCTCTCCTAT CTTCTGCACC ACGCGTTAAG TCgacaatca 1801 acctctggat tacaaaattt gtgaaagatt