Transcript: Mouse NM_001190179.1

Mus musculus nucleoporin 35 (Nup35), transcript variant 2, mRNA.

Source:
NCBI, updated 2017-06-11
Taxon:
Mus musculus (mouse)
Gene:
Nup35 (69482)
Length:
2752
CDS:
153..1079

Additional Resources:

NCBI RefSeq record:
NM_001190179.1
NBCI Gene record:
Nup35 (69482)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001190179.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000352664 ACTCCGCAACCTCGATCAATT pLKO_005 243 CDS 100% 13.200 18.480 N Nup35 n/a
2 TRCN0000192441 CCATCTGTAGCTTGCACTTTA pLKO.1 1219 3UTR 100% 13.200 18.480 N Nup35 n/a
3 TRCN0000342150 CCATCTGTAGCTTGCACTTTA pLKO_005 1219 3UTR 100% 13.200 18.480 N Nup35 n/a
4 TRCN0000201892 CTCCGCAACCTCGATCAATTA pLKO.1 244 CDS 100% 13.200 18.480 N Nup35 n/a
5 TRCN0000215747 GTCAAGCCATGTATAGATAAA pLKO.1 816 CDS 100% 13.200 18.480 N Nup35 n/a
6 TRCN0000191786 GCATATTCGTTACCAATCTAA pLKO.1 731 CDS 100% 5.625 7.875 N Nup35 n/a
7 TRCN0000342149 GCATATTCGTTACCAATCTAA pLKO_005 731 CDS 100% 5.625 7.875 N Nup35 n/a
8 TRCN0000191966 GTTTGCACAATATGGGAATAT pLKO.1 674 CDS 100% 13.200 10.560 N Nup35 n/a
9 TRCN0000191878 GAGAAGTATCTATGATGACAT pLKO.1 374 CDS 100% 4.950 3.960 N Nup35 n/a
10 TRCN0000342148 GAGAAGTATCTATGATGACAT pLKO_005 374 CDS 100% 4.950 3.960 N Nup35 n/a
11 TRCN0000192201 CTGAACCAATGATGTTGGGTT pLKO.1 145 5UTR 100% 2.640 2.112 N Nup35 n/a
12 TRCN0000342151 TAGTGACTACCAGGTTATTTC pLKO_005 989 CDS 100% 13.200 9.240 N Nup35 n/a
13 TRCN0000202161 CCGTGATAGACCTGAAGAGAA pLKO.1 1248 3UTR 100% 4.950 3.465 N Nup35 n/a
14 TRCN0000202394 GTCCTCTTGTTGGAGCTACAA pLKO.1 463 CDS 100% 4.950 3.465 N Nup35 n/a
15 TRCN0000190167 CCTTTACAACACCCATCAGGA pLKO.1 886 CDS 100% 2.640 1.848 N Nup35 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001190179.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04852 pDONR223 100% 83.5% 88.6% None (many diffs) n/a
2 ccsbBroad304_04852 pLX_304 0% 83.5% 88.6% V5 (many diffs) n/a
3 TRCN0000466533 GCGCATGGCTTCGAAATGATGGTG pLX_317 36.1% 83.5% 88.6% V5 (many diffs) n/a
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