Transcript: Human NM_001195733.2

Homo sapiens phosphatidylinositol-4-phosphate 5-kinase type 1 gamma (PIP5K1C), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-09-29
Taxon:
Homo sapiens (human)
Gene:
PIP5K1C (23396)
Length:
4985
CDS:
79..2001

Additional Resources:

NCBI RefSeq record:
NM_001195733.2
NBCI Gene record:
PIP5K1C (23396)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001145164 GGGTTGGACAGCTCGATCAG pXPR_003 CGG 492 26% 6 0.4825 PIP5K1C PIP5K1C 77667
2 BRDN0001145736 TGTTGTTCATGACCACGACG pXPR_003 CGG 704 37% 7 0.2041 PIP5K1C PIP5K1C 77666
3 BRDN0001148198 GGGAAGAAGTTGGGCCATCG pXPR_003 AGG 179 9% 3 -0.6450 PIP5K1C PIP5K1C 77665
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001195733.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000363153 ATCGCATTTGAGTCCTTATTT pLKO_005 2239 3UTR 100% 15.000 21.000 N PIP5K1C n/a
2 TRCN0000194679 CAATCGCATTTGAGTCCTTAT pLKO.1 2237 3UTR 100% 10.800 15.120 N PIP5K1C n/a
3 TRCN0000037668 CAACACGGTCTTTCGGAAGAA pLKO.1 1398 CDS 100% 4.950 6.930 N PIP5K1C n/a
4 TRCN0000037665 CGGCGAAACCACCTACAAGAA pLKO.1 276 CDS 100% 4.950 6.930 N PIP5K1C n/a
5 TRCN0000199143 CCAACAGAGGTTCTGTCCATG pLKO.1 196 CDS 100% 4.050 5.670 N PIP5K1C n/a
6 TRCN0000195424 CTTTCGAAGAAGCCACTACAG pLKO.1 1622 CDS 100% 4.050 5.670 N PIP5K1C n/a
7 TRCN0000037666 CGTGGTCAAGATGCACCTCAA pLKO.1 810 CDS 100% 4.050 3.240 N PIP5K1C n/a
8 TRCN0000037664 GCAGTCCTACAGGTTCATCAA pLKO.1 1278 CDS 100% 4.950 3.465 N PIP5K1C n/a
9 TRCN0000199413 CGCCACCTTCTTTCGAAGAAG pLKO.1 1613 CDS 100% 0.495 0.347 N PIP5K1C n/a
10 TRCN0000363133 TCATGAGCAACACGGTCTTTC pLKO_005 1391 CDS 100% 10.800 6.480 N PIP5K1C n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001195733.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

No results found.