Transcript: Human NM_001204066.2

Homo sapiens ankyrin repeat and sterile alpha motif domain containing 1B (ANKS1B), transcript variant 5, mRNA.

Source:
NCBI, updated 2019-09-29
Taxon:
Homo sapiens (human)
Gene:
ANKS1B (56899)
Length:
3035
CDS:
197..1270

Additional Resources:

NCBI RefSeq record:
NM_001204066.2
NBCI Gene record:
ANKS1B (56899)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001204066.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000234648 TGAGCATGAAATTCGTAATAT pLKO_005 868 CDS 100% 15.000 12.000 N ANKS1B n/a
2 TRCN0000167412 GTCGTGTGATTACAAAGCTTT pLKO.1 664 CDS 100% 4.950 3.960 N ANKS1B n/a
3 TRCN0000234649 CTCTCAACATTTGCCTATATC pLKO_005 917 CDS 100% 13.200 9.240 N ANKS1B n/a
4 TRCN0000234647 TGAAGAAGGTCCCTACTATTA pLKO_005 786 CDS 100% 13.200 9.240 N ANKS1B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001204066.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15926 pDONR223 0% 99.9% 99.7% None 974C>G n/a
2 ccsbBroad304_15926 pLX_304 0% 99.9% 99.7% V5 974C>G n/a
3 TRCN0000481009 CTCTTATTGAGGCACTTGCCAGAA pLX_317 37.9% 99.9% 99.7% V5 974C>G n/a
4 ccsbBroadEn_12309 pDONR223 100% 75.4% 75.4% None 1_45del;284_285ins285;965_966insGCA n/a
5 ccsbBroad304_12309 pLX_304 0% 75.4% 75.4% V5 1_45del;284_285ins285;965_966insGCA n/a
6 TRCN0000474562 TCACTGTACAGTACAGTCAGCCCA pLX_317 16.7% 75.4% 75.4% V5 1_45del;284_285ins285;965_966insGCA n/a
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