Transcript: Human NM_001253693.1

Homo sapiens mucolipin 3 (MCOLN3), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-08-06
Taxon:
Homo sapiens (human)
Gene:
MCOLN3 (55283)
Length:
2699
CDS:
148..1641

Additional Resources:

NCBI RefSeq record:
NM_001253693.1
NBCI Gene record:
MCOLN3 (55283)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001253693.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000416641 TTGACAATCATTGGATCAATT pLKO_005 1030 CDS 100% 13.200 10.560 N MCOLN3 n/a
2 TRCN0000428091 GATGACCCTCCAGTATCTTTA pLKO_005 1600 CDS 100% 13.200 9.240 N MCOLN3 n/a
3 TRCN0000074311 CCATGGAAACTTGCCATACAA pLKO.1 325 CDS 100% 5.625 3.938 N MCOLN3 n/a
4 TRCN0000074309 CTGGCATGTATCTGGATCAAT pLKO.1 792 CDS 100% 5.625 3.938 N MCOLN3 n/a
5 TRCN0000074310 GAACACTCATTACATGATGAT pLKO.1 819 CDS 100% 4.950 3.465 N MCOLN3 n/a
6 TRCN0000074308 GCACCAGCTAACAATGTGATA pLKO.1 2171 3UTR 100% 4.950 3.465 N MCOLN3 n/a
7 TRCN0000430431 GAGTGGGTGTAGTTATCATTT pLKO_005 2066 3UTR 100% 13.200 7.920 N MCOLN3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001253693.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12197 pDONR223 100% 47.6% 46.4% None (many diffs) n/a
2 ccsbBroad304_12197 pLX_304 0% 47.6% 46.4% V5 (many diffs) n/a
3 TRCN0000478835 GCCATGTGATAGAGAATTGTTCGG pLX_317 45.7% 47.6% 46.4% V5 (many diffs) n/a
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