Construct: ORF ccsbBroad304_12197

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018103.1_s304c1, BRDN0000400593
Derived from:
ccsbBroadEn_12197
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MCOLN3 (55283)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_12197
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55283 MCOLN3 mucolipin 3 XM_011541741.2 71.7% 70.1% (many diffs)
2 human 55283 MCOLN3 mucolipin 3 NM_018298.11 57.7% 56.5% (many diffs)
3 human 55283 MCOLN3 mucolipin 3 XM_005271003.1 57.7% 56.5% (many diffs)
4 human 55283 MCOLN3 mucolipin 3 XM_006710750.1 57.7% 56.5% (many diffs)
5 human 55283 MCOLN3 mucolipin 3 XM_011541739.2 56.4% 55.2% (many diffs)
6 human 55283 MCOLN3 mucolipin 3 NM_001253693.1 47.6% 46.4% (many diffs)
7 human 55283 MCOLN3 mucolipin 3 XM_011541740.2 45.6% 44.5% (many diffs)
8 human 55283 MCOLN3 mucolipin 3 XM_011541742.1 29.7% 28.7% (many diffs)
9 human 55283 MCOLN3 mucolipin 3 XR_946699.2 15.5% (many diffs)
10 mouse 171166 Mcoln3 mucolipin 3 XM_006501085.2 55.6% 57.3% (many diffs)
11 mouse 171166 Mcoln3 mucolipin 3 NM_134160.1 48.7% 50.2% (many diffs)
12 mouse 171166 Mcoln3 mucolipin 3 XM_006501081.2 48.7% 50.2% (many diffs)
13 mouse 171166 Mcoln3 mucolipin 3 XM_006501082.3 48.7% 50.2% (many diffs)
14 mouse 171166 Mcoln3 mucolipin 3 XM_006501083.3 48.7% 50.2% (many diffs)
15 mouse 171166 Mcoln3 mucolipin 3 XM_006501084.3 48.7% 50.2% (many diffs)
16 mouse 171166 Mcoln3 mucolipin 3 XM_006501086.3 36% 37.7% (many diffs)
17 mouse 171166 Mcoln3 mucolipin 3 XM_006501087.3 36% 37.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1029
ORF length:
963
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc agatcctgag gtagttgtga gtagctgcag ctctcatgaa gaggaaaatc
121gctgcaattt taaccagcaa acatctccat ctgaggagct tctattagaa gaccagatga
181ggcgaaaact caaatttttt ttcatgaatc cctgtgagaa gttctgggct cgaggtagaa
241aaccatggaa acttgccata caaattctaa aaattgcaat ggtgactatc cagctggtct
301tatttgggct aagtaaccag atggtggtag ctttcaagga agagaatact atagcattca
361aacacctttt cctaaaagga tatatggacc gaatggatga cacatatgca gtgtacacac
421aaagtgacgt gtatgatcag ttaatcttcg cagtaaacca gtacttgcag ctatacaatg
481tctccgttgg gaatcatgct tatgagaaca aaggtaccaa gcaatctgct atggcaatct
541gtcagcactt atacaagcga ggaaacatct accctggaaa tgataccttt gacatcgatc
601cagaaattga aactgagtgt ttctttgtgg agccagatga accttttcac attgggacac
661cagcagaaaa taaactgaac ttaacactgg acttccacag actcctaaca gtggagcttc
721agtttaaact gaaagccatt aatctgcaga cagttcgtca tcaagaactc cctgactgtt
781atgactttac tctgactata acatttgaca acaaggccca tagtggaaga attaaaataa
841gtttagataa tgacatttcc atcagagaat gtaaagactg gcatgtatct ggatcaattc
901agaagaacac tcattacatg atgatctttg atgcctttgt cattctgact tgcttggttt
961cattaatcct ctgcattaga tctgtgatta gaggacttca gcttcagcag gtagggaacg
1021ttgctttcta cccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc
1081tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa
1141tcaacctctg gatta

Download FASTA (ORF) (Full)