Transcript: Mouse NM_001253718.1

Mus musculus autophagy related 7 (Atg7), transcript variant 2, mRNA.

Source:
NCBI, updated 2017-06-14
Taxon:
Mus musculus (mouse)
Gene:
Atg7 (74244)
Length:
3895
CDS:
195..2291

Additional Resources:

NCBI RefSeq record:
NM_001253718.1
NBCI Gene record:
Atg7 (74244)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001253718.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000305991 TTCTGTCACGGTTCGATAATG pLKO_005 2065 CDS 100% 13.200 18.480 N Atg7 n/a
2 TRCN0000092165 CGTGACACATAGCATCATCTT pLKO.1 1016 CDS 100% 4.950 3.960 N Atg7 n/a
3 TRCN0000375421 TCTTACCCTGCTCCATCAAGA pLKO_005 2222 CDS 100% 4.950 3.960 N Atg7 n/a
4 TRCN0000092163 CCAGCTCTGAACTCAATAATA pLKO.1 2453 3UTR 100% 15.000 10.500 N Atg7 n/a
5 TRCN0000305993 GCAGTGATGACCGCATGAATG pLKO_005 1999 CDS 100% 10.800 7.560 N Atg7 n/a
6 TRCN0000305928 GTCCTTCCATGTGCACTAATC pLKO_005 2505 3UTR 100% 10.800 7.560 N Atg7 n/a
7 TRCN0000092164 GCCTGGCATTTGATAAATGTA pLKO.1 2098 CDS 100% 5.625 3.938 N Atg7 n/a
8 TRCN0000375444 GCCAACATCCCTGGATACAAG pLKO_005 1800 CDS 100% 4.950 3.465 N Atg7 n/a
9 TRCN0000092166 CCTAAAGAAGTACCACTTCTA pLKO.1 596 CDS 100% 0.495 0.347 N Atg7 n/a
10 TRCN0000325414 CCTAAAGAAGTACCACTTCTA pLKO_005 596 CDS 100% 0.495 0.347 N Atg7 n/a
11 TRCN0000092167 CCAAGGTCAAAGGACAAAGAT pLKO.1 842 CDS 100% 5.625 3.375 N Atg7 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001253718.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02460 pDONR223 100% 82.3% 88.7% None (many diffs) n/a
2 ccsbBroad304_02460 pLX_304 0% 82.3% 88.7% V5 (many diffs) n/a
3 TRCN0000472530 ACCACTTTTGACACCTGTACTAAC pLX_317 21.4% 82.3% 88.7% V5 (many diffs) n/a
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