Construct: ORF ccsbBroad304_02460
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007554.1_s304c1, BRDN0000409255
- Derived from:
- ccsbBroadEn_02460
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ATG7 (10533)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02460
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10533 | ATG7 | autophagy related 7 | NM_001136031.3 | 100% | 100% | |
| 2 | human | 10533 | ATG7 | autophagy related 7 | XM_011533286.2 | 100% | 100% | |
| 3 | human | 10533 | ATG7 | autophagy related 7 | XM_017005551.1 | 98.9% | 98.6% | (many diffs) |
| 4 | human | 10533 | ATG7 | autophagy related 7 | NM_001349232.2 | 96.1% | 96.1% | 1876_1956del |
| 5 | human | 10533 | ATG7 | autophagy related 7 | NM_001349233.2 | 96.1% | 96.1% | 1876_1956del |
| 6 | human | 10533 | ATG7 | autophagy related 7 | NM_001349234.2 | 96.1% | 96.1% | 1876_1956del |
| 7 | human | 10533 | ATG7 | autophagy related 7 | NM_001349235.2 | 96.1% | 96.1% | 1876_1956del |
| 8 | human | 10533 | ATG7 | autophagy related 7 | NM_006395.3 | 96.1% | 96.1% | 1876_1956del |
| 9 | human | 10533 | ATG7 | autophagy related 7 | XM_011533283.2 | 95.1% | 94.8% | (many diffs) |
| 10 | human | 10533 | ATG7 | autophagy related 7 | XM_017005548.1 | 95.1% | 94.8% | (many diffs) |
| 11 | human | 10533 | ATG7 | autophagy related 7 | XM_017005547.2 | 94.9% | 94.2% | (many diffs) |
| 12 | human | 10533 | ATG7 | autophagy related 7 | XM_011533284.2 | 94.7% | 94.7% | 1876_1956del;2080_2081delCAinsAT;2082_2083ins27 |
| 13 | human | 10533 | ATG7 | autophagy related 7 | XM_011533281.2 | 94.5% | 93.1% | (many diffs) |
| 14 | human | 10533 | ATG7 | autophagy related 7 | XM_011533280.2 | 94.2% | 93.1% | (many diffs) |
| 15 | human | 10533 | ATG7 | autophagy related 7 | XM_017005543.1 | 94.2% | 93.1% | (many diffs) |
| 16 | human | 10533 | ATG7 | autophagy related 7 | XM_011533279.2 | 91.7% | 90.7% | (many diffs) |
| 17 | human | 10533 | ATG7 | autophagy related 7 | XM_024453312.1 | 91.7% | 90.7% | (many diffs) |
| 18 | human | 10533 | ATG7 | autophagy related 7 | XM_006712931.4 | 90.7% | 89.7% | (many diffs) |
| 19 | human | 10533 | ATG7 | autophagy related 7 | XM_006712932.4 | 90.7% | 89.7% | (many diffs) |
| 20 | human | 10533 | ATG7 | autophagy related 7 | XM_006712933.3 | 90.7% | 89.7% | (many diffs) |
| 21 | human | 10533 | ATG7 | autophagy related 7 | XM_011533277.3 | 90.7% | 89.7% | (many diffs) |
| 22 | human | 10533 | ATG7 | autophagy related 7 | XM_011533278.2 | 90.7% | 89.7% | (many diffs) |
| 23 | human | 10533 | ATG7 | autophagy related 7 | XM_017005542.1 | 90.7% | 89.7% | (many diffs) |
| 24 | human | 10533 | ATG7 | autophagy related 7 | NM_001349236.2 | 90.6% | 90.6% | 411_412ins117;1759_1839del |
| 25 | human | 10533 | ATG7 | autophagy related 7 | NM_001144912.2 | 90.2% | 88.4% | (many diffs) |
| 26 | human | 10533 | ATG7 | autophagy related 7 | NM_001349237.2 | 88.6% | 88.6% | 1123_1124ins159;1717_1797del |
| 27 | human | 10533 | ATG7 | autophagy related 7 | XM_017005550.1 | 84.5% | 83.5% | (many diffs) |
| 28 | human | 10533 | ATG7 | autophagy related 7 | XM_011533285.3 | 83.6% | 82.6% | (many diffs) |
| 29 | human | 10533 | ATG7 | autophagy related 7 | XM_017005549.2 | 83.6% | 82.6% | (many diffs) |
| 30 | human | 10533 | ATG7 | autophagy related 7 | XR_001739980.2 | 80.1% | (many diffs) | |
| 31 | human | 10533 | ATG7 | autophagy related 7 | XR_940363.2 | 69% | (many diffs) | |
| 32 | human | 10533 | ATG7 | autophagy related 7 | XM_024453315.1 | 51.9% | 51.9% | 0_1ins933;943_1023del |
| 33 | human | 10533 | ATG7 | autophagy related 7 | NM_001349238.2 | 50.9% | 50.2% | (many diffs) |
| 34 | human | 10533 | ATG7 | autophagy related 7 | XM_024453313.1 | 48.7% | 47.9% | (many diffs) |
| 35 | human | 10533 | ATG7 | autophagy related 7 | XM_024453314.1 | 48.7% | 47.9% | (many diffs) |
| 36 | mouse | 74244 | Atg7 | autophagy related 7 | NM_001253718.1 | 82.3% | 88.7% | (many diffs) |
| 37 | mouse | 74244 | Atg7 | autophagy related 7 | NM_028835.4 | 82.3% | 88.7% | (many diffs) |
| 38 | mouse | 74244 | Atg7 | autophagy related 7 | XM_006506709.2 | 82.3% | 88.7% | (many diffs) |
| 39 | mouse | 74244 | Atg7 | autophagy related 7 | XM_006506710.3 | 82.3% | 88.7% | (many diffs) |
| 40 | mouse | 74244 | Atg7 | autophagy related 7 | XM_011241494.2 | 82.3% | 88.7% | (many diffs) |
| 41 | mouse | 74244 | Atg7 | autophagy related 7 | XM_011241495.2 | 82.3% | 88.7% | (many diffs) |
| 42 | mouse | 74244 | Atg7 | autophagy related 7 | XM_006506708.3 | 81.3% | 87.4% | (many diffs) |
| 43 | mouse | 74244 | Atg7 | autophagy related 7 | NM_001253717.1 | 78.3% | 84.2% | (many diffs) |
| 44 | mouse | 74244 | Atg7 | autophagy related 7 | XM_006506707.2 | 78.3% | 84.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2094
- ORF length:
- 2028
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcagctacg ggggatcctg gactctctaa actgcagttt gcccctttta 121 gtagtgcctt ggatgttggg ttttggcatg agttgaccca gaagaagctg aacgagtatc 181 ggctggatga agctcccaag gacattaagg gttattacta caatggtgac tctgctgggc 241 tgccagctcg cttaacattg gagttcagtg cttttgacat gagtgctccc accccagccc 301 gttgctgccc agctattgga acactgtata acaccaacac actcgagtct ttcaagactg 361 cagataagaa gctccttttg gaacaagcag caaatgagat atgggaatcc ataaaatcag 421 gcactgctct tgaaaaccct gtactcctca acaagttcct cctcttgaca tttgcagatc 481 taaagaagta ccacttctac tattggtttt gctatcctgc cctctgtctt ccagagagtt 541 tacctctcat tcaggggcca gtgggtttgg atcaaaggtt ttcactaaaa cagattgaag 601 cactagagtg tgcatatgat aatctttgtc aaacagaagg agtcacagct cttccttact 661 tcttaatcaa gtatgatgag aacatggtgc tggtttcctt gcttaaacac tacagtgatt 721 tcttccaagg tcaaaggacg aagataacaa ttggtgtata tgatccctgt aacttagccc 781 agtaccctgg atggcctttg aggaattttt tggtcctagc agcccacaga tggagtagca 841 gtttccagtc tgttgaagtt gtttgcttcc gtgaccgtac catgcagggg gcgagagacg 901 ttgcccacag catcatcttc gaagtgaagc ttccagaaat ggcatttagc ccagattgtc 961 ctaaagcagt tggatgggaa aagaaccaga aaggaggcat gggaccaagg atggtgaacc 1021 tcagtgaatg tatggaccct aaaaggttag ctgagtcatc agtggatcta aatctcaaac 1081 tgatgtgttg gagattggtt cctactttag acttggacaa ggttgtgtct gtcaaatgtc 1141 tgctgcttgg agccggcacc ttgggttgca atgtagctag gacgttgatg ggttggggcg 1201 tgagacacat cacatttgtg gacaatgcca agatctccta ctccaatcct gtgaggcagc 1261 ctctctatga gtttgaagat tgcctagggg gtggtaagcc caaggctctg gcagcagcgg 1321 accggctcca gaaaatattc cccggtgtga atgccagagg attcaacatg agcataccta 1381 tgcctgggca tccagtgaac ttctccagtg tcactctgga gcaagcccgc agagatgtgg 1441 agcaactgga gcagctcatc gaaagccatg atgtcgtctt cctattgatg gacaccaggg 1501 agagccggtg gcttcctgcc gtcattgctg caagcaagag aaagctggtc atcaatgctg 1561 ctttgggatt tgacacattt gttgtcatga gacatggtct gaagaaacca aagcagcaag 1621 gagctgggga cttgtgtcca aaccaccctg tggcatctgc tgacctcctg ggctcatcgc 1681 tttttgccaa catccctggt tacaagcttg gctgctactt ctgcaatgat gtggtggccc 1741 caggagattc aaccagagac cggaccttgg accagcagtg cactgtgagt cgtccaggac 1801 tggccgtgat tgcaggagcc ctggccgtgg aattgatggt atctgttttg cagcatccag 1861 aagggggcta tgccattgcc agcagcagtg acgatcggat gaatgagcct ccaacctctc 1921 ttgggcttgt gcctcaccag gttcttgatc aatatgaacg agaaggattt aacttcctag 1981 ccaaggtgtt taattcttca cattccttct tagaagactt gactggtctt acattgctgc 2041 atcaagaaac ccaagctgct gagatctggg acatgagcga tgatgagacc atctgcccaa 2101 ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta 2161 cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta