Transcript: Human NM_001256336.3

Homo sapiens chromodomain helicase DNA binding protein 1 like (CHD1L), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-05-31
Taxon:
Homo sapiens (human)
Gene:
CHD1L (9557)
Length:
2860
CDS:
214..2607

Additional Resources:

NCBI RefSeq record:
NM_001256336.3
NBCI Gene record:
CHD1L (9557)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001256336.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000013472 CGTATTGGACATGCCACGAAA pLKO.1 2437 CDS 100% 4.950 3.465 N CHD1L n/a
2 TRCN0000280395 CGTATTGGACATGCCACGAAA pLKO_005 2437 CDS 100% 4.950 3.465 N CHD1L n/a
3 TRCN0000013471 GCCAAGAGAAGGAGACTCATA pLKO.1 1867 CDS 100% 4.950 3.465 N CHD1L n/a
4 TRCN0000280441 GCCAAGAGAAGGAGACTCATA pLKO_005 1867 CDS 100% 4.950 3.465 N CHD1L n/a
5 TRCN0000013468 CCTGTCTTTAGCAACCAGCTA pLKO.1 2628 3UTR 100% 2.640 1.848 N CHD1L n/a
6 TRCN0000297839 CCTGTCTTTAGCAACCAGCTA pLKO_005 2628 3UTR 100% 2.640 1.848 N CHD1L n/a
7 TRCN0000013470 GCACAAACTCTTGCAGCCATT pLKO.1 663 CDS 100% 4.050 2.430 N CHD1L n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001256336.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11389 pDONR223 100% 84.8% 80.2% None (many diffs) n/a
2 ccsbBroad304_11389 pLX_304 0% 84.8% 80.2% V5 (many diffs) n/a
3 TRCN0000479276 GCTGATGTGCAGGCAAGTTCTTGA pLX_317 18% 84.8% 80.2% V5 (many diffs) n/a
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