Construct: ORF TRCN0000479276
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017355.1_s317c1
- Derived from:
- ccsbBroadEn_11389
- DNA Barcode:
- GCTGATGTGCAGGCAAGTTCTTGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CHD1L (9557)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479276
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9557 | CHD1L | chromodomain helicase DNA b... | NM_001256338.3 | 99.9% | 100% | 657A>G |
| 2 | human | 9557 | CHD1L | chromodomain helicase DNA b... | NM_001348454.2 | 92.9% | 93% | 125_280del;813A>G |
| 3 | human | 9557 | CHD1L | chromodomain helicase DNA b... | NM_001348455.2 | 92% | 86.9% | (many diffs) |
| 4 | human | 9557 | CHD1L | chromodomain helicase DNA b... | NM_001256337.3 | 88.8% | 88.8% | 0_1ins231;426A>G |
| 5 | human | 9557 | CHD1L | chromodomain helicase DNA b... | NM_001348456.2 | 88.8% | 88.8% | 0_1ins231;426A>G |
| 6 | human | 9557 | CHD1L | chromodomain helicase DNA b... | NM_001348457.2 | 88.8% | 88.8% | 0_1ins231;426A>G |
| 7 | human | 9557 | CHD1L | chromodomain helicase DNA b... | NM_001348458.2 | 88.8% | 88.8% | 0_1ins231;426A>G |
| 8 | human | 9557 | CHD1L | chromodomain helicase DNA b... | NM_001348459.2 | 88.8% | 88.8% | 0_1ins231;426A>G |
| 9 | human | 9557 | CHD1L | chromodomain helicase DNA b... | NM_001348460.2 | 88.8% | 88.8% | 0_1ins231;426A>G |
| 10 | human | 9557 | CHD1L | chromodomain helicase DNA b... | NM_001348461.2 | 88.8% | 88.8% | 0_1ins231;426A>G |
| 11 | human | 9557 | CHD1L | chromodomain helicase DNA b... | NM_001348462.2 | 88.8% | 88.8% | 0_1ins231;426A>G |
| 12 | human | 9557 | CHD1L | chromodomain helicase DNA b... | NM_001348463.2 | 88.8% | 88.8% | 0_1ins231;426A>G |
| 13 | human | 9557 | CHD1L | chromodomain helicase DNA b... | NM_001348464.2 | 88.8% | 88.8% | 0_1ins231;426A>G |
| 14 | human | 9557 | CHD1L | chromodomain helicase DNA b... | NM_001348465.2 | 88.8% | 88.8% | 0_1ins231;426A>G |
| 15 | human | 9557 | CHD1L | chromodomain helicase DNA b... | NM_001348466.2 | 88.8% | 88.8% | 0_1ins231;426A>G |
| 16 | human | 9557 | CHD1L | chromodomain helicase DNA b... | NM_001348453.2 | 86.2% | 81.5% | (many diffs) |
| 17 | human | 9557 | CHD1L | chromodomain helicase DNA b... | NM_024568.4 | 86.2% | 81.5% | (many diffs) |
| 18 | human | 9557 | CHD1L | chromodomain helicase DNA b... | NM_001256336.3 | 84.8% | 80.2% | (many diffs) |
| 19 | human | 9557 | CHD1L | chromodomain helicase DNA b... | NM_001348451.2 | 81.9% | 77.5% | (many diffs) |
| 20 | human | 9557 | CHD1L | chromodomain helicase DNA b... | NM_001348452.1 | 81.9% | 77.5% | (many diffs) |
| 21 | human | 9557 | CHD1L | chromodomain helicase DNA b... | NR_145693.2 | 81.1% | (many diffs) | |
| 22 | human | 9557 | CHD1L | chromodomain helicase DNA b... | NR_145690.2 | 79.5% | (many diffs) | |
| 23 | human | 9557 | CHD1L | chromodomain helicase DNA b... | NM_004284.6 | 77.2% | 77.2% | 125_736del;1269A>G |
| 24 | human | 9557 | CHD1L | chromodomain helicase DNA b... | NR_145687.2 | 73.8% | (many diffs) | |
| 25 | human | 9557 | CHD1L | chromodomain helicase DNA b... | NR_145695.2 | 72.2% | (many diffs) | |
| 26 | human | 9557 | CHD1L | chromodomain helicase DNA b... | NR_145681.2 | 72.1% | (many diffs) | |
| 27 | human | 9557 | CHD1L | chromodomain helicase DNA b... | NR_145688.2 | 71% | (many diffs) | |
| 28 | human | 9557 | CHD1L | chromodomain helicase DNA b... | NR_145684.2 | 70.3% | (many diffs) | |
| 29 | human | 9557 | CHD1L | chromodomain helicase DNA b... | NR_145689.2 | 70.2% | (many diffs) | |
| 30 | human | 9557 | CHD1L | chromodomain helicase DNA b... | NR_145685.2 | 69.6% | (many diffs) | |
| 31 | human | 9557 | CHD1L | chromodomain helicase DNA b... | NR_145692.2 | 69.4% | (many diffs) | |
| 32 | human | 9557 | CHD1L | chromodomain helicase DNA b... | NR_145691.2 | 68.8% | (many diffs) | |
| 33 | human | 9557 | CHD1L | chromodomain helicase DNA b... | XM_024451049.1 | 67.8% | 67.8% | 0_1ins669 |
| 34 | human | 9557 | CHD1L | chromodomain helicase DNA b... | XM_024451050.1 | 67.8% | 67.8% | 0_1ins669 |
| 35 | human | 9557 | CHD1L | chromodomain helicase DNA b... | XM_024451051.1 | 67.8% | 67.8% | 0_1ins669 |
| 36 | human | 9557 | CHD1L | chromodomain helicase DNA b... | NR_145683.2 | 67.7% | (many diffs) | |
| 37 | human | 9557 | CHD1L | chromodomain helicase DNA b... | NR_145682.2 | 67.5% | (many diffs) | |
| 38 | human | 9557 | CHD1L | chromodomain helicase DNA b... | NR_046070.3 | 63.8% | (many diffs) | |
| 39 | human | 9557 | CHD1L | chromodomain helicase DNA b... | NR_145694.2 | 63.7% | (many diffs) | |
| 40 | human | 9557 | CHD1L | chromodomain helicase DNA b... | XR_001737550.2 | 63.6% | (many diffs) | |
| 41 | human | 9557 | CHD1L | chromodomain helicase DNA b... | NR_145686.2 | 63.5% | (many diffs) | |
| 42 | mouse | 68058 | Chd1l | chromodomain helicase DNA b... | XM_017319700.1 | 73.1% | 71.9% | (many diffs) |
| 43 | mouse | 68058 | Chd1l | chromodomain helicase DNA b... | NM_026539.2 | 64.5% | 63.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2145
- ORF length:
- 2079
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gcgcgcgggc gctactagcc gcgggggcca agcccctggc ttcttactgc 121 ggcttcatac tgagggccga gccgaggcgg cgcgggtgca ggagcaggac ttacggcagt 181 gggggctgac agcaagtgaa ctgcacaaac tcttgcagcc atttctgctg aggcgagtga 241 aagctgaggt agctacagag cttcccaaga agacagaagt agtgatatac catggcatgt 301 cagcattgca gaagaaatac tacaaggcca ttttgatgaa agacctagat gcatttgaaa 361 atgagacggc aaagaaagtt aaactacaga acattttgtc ccagcttcga aagtgtgtgg 421 atcacccata tttgtttgat ggtgtggagc cggagccttt tgaagttgga gaccacctga 481 ctgaggctag tgggaagctt cacctgctgg ataagctact agcattcctg tattctgggg 541 gccatcgggt tttacttttc tcccaaatga cccagatgtt ggatattctc caagactata 601 tggattacag aggctacagc tatgagcgtg tggatggttc tgtgagagga gaagagagac 661 acttggccat taagaacttt ggacagcagc ccattttcgt ttttctcctg agtactaggg 721 cgggtggagt tggcatgaac ttaacagcag cagatactgt gatttttgtt gacagtgact 781 ttaatcctca gaatgacttg caagcagctg ccagggctca tcgcattggc caaaacaagt 841 ctgttaaagt tattcggctg attggtcgag acactgtgga agaaatagtc tataggaaag 901 cagcctccaa actgcagctc accaacatga tcatagaagg aggccatttt actctgggag 961 cccagaaacc cgctgccgat gctgacctcc agttgagtga gatactcaaa tttggtttgg 1021 ataaactgct ggcctctgag gggagcacca tggatgaaat agacctggag tccatcctgg 1081 gagaaacaaa agatggccag tgggtctctg atgccttgcc tgcagcagaa ggagggagca 1141 gagatcaaga ggaaggaaaa aatcatatgt acttatttga aggtaaagat tattctaaag 1201 agcccagtaa ggaagacaga aaatcatttg aacaactggt aaaccttcag aaaacccttt 1261 tggagaaagc tagtcaagag ggccgatcac tccgaaataa aggcagtgtt ctcatcccag 1321 gccttgtgga gggatctacc aaaaggaagc gggttctgag tccagaagag ctggaggaca 1381 gacagaagaa aagacaagaa gcagctgcca agagaaggag actcatagag gagaagaaga 1441 ggcaaaagga agaggctgaa cataagaaaa agatggcctg gtgggaatcc aacaattacc 1501 agtccttctg cctgccctct gaggagagcg agccagagga ccttgagaat ggggaagaga 1561 gctctgctga gctggattac caagacccag atgctacttc cctcaagtac gttagtggtg 1621 atgtcaccca ccctcaggct ggggccgagg atgctctcat tgtgcactgc gtagatgact 1681 ctggccactg gggcagaggt ggtttattta cagctctgga aaagcgatcc gctgagccaa 1741 gaaaaatata tgagctggct gggaaaatga aagaccTGAG TTTGGGAGGT GTCCTTTTAT 1801 TTCCTGTTGA TGATAAAGAA TCAAGAAACA AAGGGCAAGA TTTGTTGGCC TTGATTGTGG 1861 CTCAGCATCG TGATCGTTCC AATGTCCTGT CTGGCATTAA GATGGCAGCC CTAGAAGAGG 1921 GCCTGAAGAA GATATTTTTA GCAGCAAAAA AGAAGAAAGC AAGTGTTCAT CTTCCACGTA 1981 TTGGACATGC CACGAAAGGT TTTAACTGGT ATGGTACTGA GCGACTTATT CGGAAACATC 2041 TGGCTGCAAG AGGCATCCCA ACTTACATAT ATTATTTTCC TAGAAGCAAG TCTGCTGTCC 2101 TTCATTCACA GTCTTCATCT TCCTCCTCAA GACAGCTGGT GCCTTACCCA ACTTTCTTGT 2161 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 2221 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 2281 GAAAGGACGA GCTGATGTGC AGGCAAGTTC TTGAACGCGT TAAGTCgaca atcaacctct 2341 ggattacaaa atttgtgaaa gatt