Construct: ORF TRCN0000468097
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012049.1_s317c1
- Derived from:
- ccsbBroadEn_08974
- DNA Barcode:
- GAAGCAGCAAATCCCAAGTAACAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ALG13 (79868)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468097
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79868 | ALG13 | ALG13 UDP-N-acetylglucosami... | NM_018466.6 | 99.7% | 99.3% | 495A>T |
| 2 | human | 79868 | ALG13 | ALG13 UDP-N-acetylglucosami... | NM_001324290.2 | 98.6% | 98.2% | 243_248delAGGTAA;501A>T |
| 3 | human | 79868 | ALG13 | ALG13 UDP-N-acetylglucosami... | NM_001039210.5 | 83.6% | 41.2% | 182_183ins59;414_415ins22 |
| 4 | human | 79868 | ALG13 | ALG13 UDP-N-acetylglucosami... | NM_001168385.3 | 79.5% | 78.7% | (many diffs) |
| 5 | human | 79868 | ALG13 | ALG13 UDP-N-acetylglucosami... | XR_001755728.1 | 66.1% | 1_40del;535_747delinsT | |
| 6 | human | 79868 | ALG13 | ALG13 UDP-N-acetylglucosami... | XR_001755727.1 | 63.7% | 1_40del;535_775delinsT | |
| 7 | human | 79868 | ALG13 | ALG13 UDP-N-acetylglucosami... | NM_001257241.3 | 52.3% | 50.3% | (many diffs) |
| 8 | human | 79868 | ALG13 | ALG13 UDP-N-acetylglucosami... | XR_001755726.1 | 42.9% | 1_40del;535_1149delinsT | |
| 9 | human | 79868 | ALG13 | ALG13 UDP-N-acetylglucosami... | NM_001257235.3 | 36.7% | 36.3% | 0_1ins312;183A>T |
| 10 | human | 79868 | ALG13 | ALG13 UDP-N-acetylglucosami... | NM_001257239.3 | 36.7% | 36.3% | 0_1ins312;183A>T |
| 11 | human | 79868 | ALG13 | ALG13 UDP-N-acetylglucosami... | NM_001257240.3 | 36.7% | 36.3% | 0_1ins312;183A>T |
| 12 | human | 79868 | ALG13 | ALG13 UDP-N-acetylglucosami... | NM_001324291.2 | 36.7% | 36.3% | 0_1ins312;183A>T |
| 13 | human | 79868 | ALG13 | ALG13 UDP-N-acetylglucosami... | NM_001324294.2 | 36.7% | 36.3% | 0_1ins312;183A>T |
| 14 | human | 79868 | ALG13 | ALG13 UDP-N-acetylglucosami... | XM_024452449.1 | 16.8% | 14.9% | (many diffs) |
| 15 | human | 79868 | ALG13 | ALG13 UDP-N-acetylglucosami... | XM_017029850.2 | 16.6% | 14.7% | (many diffs) |
| 16 | human | 79868 | ALG13 | ALG13 UDP-N-acetylglucosami... | XM_017029849.1 | 16.4% | 14.5% | (many diffs) |
| 17 | human | 79868 | ALG13 | ALG13 UDP-N-acetylglucosami... | NR_136735.2 | 16% | (many diffs) | |
| 18 | human | 79868 | ALG13 | ALG13 UDP-N-acetylglucosami... | XM_017029848.1 | 14.7% | 13% | (many diffs) |
| 19 | human | 79868 | ALG13 | ALG13 UDP-N-acetylglucosami... | NM_001324292.2 | 14.5% | 12.9% | (many diffs) |
| 20 | human | 79868 | ALG13 | ALG13 UDP-N-acetylglucosami... | XM_011531030.2 | 14.2% | 12.6% | (many diffs) |
| 21 | human | 79868 | ALG13 | ALG13 UDP-N-acetylglucosami... | NR_033125.3 | 14.1% | (many diffs) | |
| 22 | human | 79868 | ALG13 | ALG13 UDP-N-acetylglucosami... | XM_006724693.3 | 13.7% | 12.1% | (many diffs) |
| 23 | human | 79868 | ALG13 | ALG13 UDP-N-acetylglucosami... | NM_001099922.3 | 13.5% | 12% | (many diffs) |
| 24 | human | 79868 | ALG13 | ALG13 UDP-N-acetylglucosami... | XM_017029847.1 | 13.4% | 11.9% | (many diffs) |
| 25 | human | 79868 | ALG13 | ALG13 UDP-N-acetylglucosami... | XM_017029846.1 | 13.3% | 11.8% | (many diffs) |
| 26 | human | 79868 | ALG13 | ALG13 UDP-N-acetylglucosami... | XM_011531028.2 | 13.3% | 11.7% | (many diffs) |
| 27 | human | 79868 | ALG13 | ALG13 UDP-N-acetylglucosami... | NR_148693.2 | 13% | (many diffs) | |
| 28 | mouse | 67574 | Alg13 | asparagine-linked glycosyla... | NM_026247.3 | 85.5% | 86.6% | (many diffs) |
| 29 | mouse | 320302 | Glt28d2 | glycosyltransferase 28 doma... | NM_177130.3 | 85.2% | 88.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 561
- ORF length:
- 495
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gtgcgtgttt gttaccgtag ggaccaccag ctttgacgac ctcattgcgt 121 gtgtgtcggc gcccgacagt ctgcaaaaaa tcgagagcct tggttacaac cgacttatcc 181 tgcaaattgg tagaggaacg gtggtacctg aacccTTCAG TACTGAGTCG TTTACTCTGG 241 ATGTTTACAG GTACAAGGAT TCCTTGAAAG AAGACATTCA GAAAGCAGAT CTTGTTATTA 301 GTCACGCAGG TGCAGGAAGC TGTTTGGAGA CTCTGGAAAA AGGAAAGCCA CTCGTAGTGG 361 TTATAAACGA AAAGTTGATG AACAATCATC AGCTGGAACT GGCAAAGCAG CTACACAAAG 421 AGGGTCATCT CTTCTATTGT ACCTGCAGCA CGCTTCCTGG GCTGTTACAG TCAATGGACT 481 TATCAACACT GAAATGTTAT CCTCCTGGCC AGCCAGAAAA ATTTTCTGCA TTTTTGGATA 541 AAGTTGTTGG ATTACAAAAT TCCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 601 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 661 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGAAG CAGCAAATCC 721 CAAGTAACAG ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt