Transcript: Human NM_001265609.1

Homo sapiens trafficking kinesin protein 1 (TRAK1), transcript variant 4, mRNA.

Source:
NCBI, updated 2019-07-05
Taxon:
Homo sapiens (human)
Gene:
TRAK1 (22906)
Length:
4683
CDS:
302..2314

Additional Resources:

NCBI RefSeq record:
NM_001265609.1
NBCI Gene record:
TRAK1 (22906)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001265609.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000036275 CCAGATGACTAAGACATATAA pLKO.1 388 CDS 100% 15.000 21.000 N TRAK1 n/a
2 TRCN0000286856 CCAGATGACTAAGACATATAA pLKO_005 388 CDS 100% 15.000 21.000 N TRAK1 n/a
3 TRCN0000036274 GCACCACGATAAGTCCTGTAA pLKO.1 2187 CDS 100% 4.950 6.930 N TRAK1 n/a
4 TRCN0000306921 ATGGCCCATCAGGGATCTTTA pLKO_005 2417 3UTR 100% 13.200 9.240 N TRAK1 n/a
5 TRCN0000036276 GCTATCGCAAATAGTTGATTT pLKO.1 940 CDS 100% 13.200 9.240 N TRAK1 n/a
6 TRCN0000286855 GCTATCGCAAATAGTTGATTT pLKO_005 940 CDS 100% 13.200 9.240 N TRAK1 n/a
7 TRCN0000294179 TGTACTGCCTTAACGACTTTG pLKO_005 1944 CDS 100% 10.800 7.560 N TRAK1 n/a
8 TRCN0000421920 TGTACTGCCTTAACGACTTTG pLKO_005 1944 CDS 100% 10.800 7.560 N Trak1 n/a
9 TRCN0000036278 CAGTCCAGAATTACTTTCATT pLKO.1 687 CDS 100% 5.625 3.938 N TRAK1 n/a
10 TRCN0000036277 CGACAACAAGACCAACAGCAT pLKO.1 1456 CDS 100% 2.640 1.848 N TRAK1 n/a
11 TRCN0000286901 CGACAACAAGACCAACAGCAT pLKO_005 1456 CDS 100% 2.640 1.848 N TRAK1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001265609.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11645 pDONR223 100% 95.8% 95.2% None (many diffs) n/a
2 ccsbBroad304_11645 pLX_304 0% 95.8% 95.2% V5 (many diffs) n/a
3 TRCN0000479369 CTTCTCATCTAATATCCCAATAAC pLX_317 7.7% 95.8% 95.2% V5 (many diffs) n/a
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