Transcript: Human NM_001270525.2

Homo sapiens orthodenticle homeobox 2 (OTX2), transcript variant 5, mRNA.

Source:
NCBI, updated 2019-09-29
Taxon:
Homo sapiens (human)
Gene:
OTX2 (5015)
Length:
2935
CDS:
212..1105

Additional Resources:

NCBI RefSeq record:
NM_001270525.2
NBCI Gene record:
OTX2 (5015)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001270525.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000015612 CCATGACCTATACTCAGGCTT pLKO.1 762 CDS 100% 2.640 3.696 N OTX2 n/a
2 TRCN0000176356 CCACTGATTGCTTGGATTATA pLKO.1 993 CDS 100% 15.000 12.000 N Otx2 n/a
3 TRCN0000421657 GATCATTGGTTATCCTGATTT pLKO_005 1330 3UTR 100% 13.200 10.560 N OTX2 n/a
4 TRCN0000015611 GCTGGCTCAACTTCCTACTTT pLKO.1 803 CDS 100% 5.625 4.500 N OTX2 n/a
5 TRCN0000175734 GCTGACTGCTTGGATTATAAA pLKO.1 1049 CDS 100% 15.000 10.500 N Otx2 n/a
6 TRCN0000438502 AGGGTGCAGGTATGGTTTAAG pLKO_005 476 CDS 100% 13.200 9.240 N Otx2 n/a
7 TRCN0000015609 CCTCGTGGAAATTCCAGGTTT pLKO.1 1080 CDS 100% 4.950 3.465 N OTX2 n/a
8 TRCN0000015608 GCACTGAAACTTTACGACAAA pLKO.1 1869 3UTR 100% 4.950 3.465 N OTX2 n/a
9 TRCN0000419989 TAGCCAATCCTTGGTTGAATC pLKO_005 1479 3UTR 100% 10.800 6.480 N OTX2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001270525.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01128 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_01128 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000472658 TCCGCACGCTCGAAAAAACGTCGT pLX_317 51.2% 100% 100% V5 n/a
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