Construct: ORF TRCN0000472658
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018512.1_s317c1
- Derived from:
- ccsbBroadEn_01128
- DNA Barcode:
- TCCGCACGCTCGAAAAAACGTCGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- OTX2 (5015)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472658
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5015 | OTX2 | orthodenticle homeobox 2 | NM_001270525.2 | 100% | 100% | |
| 2 | human | 5015 | OTX2 | orthodenticle homeobox 2 | NM_021728.4 | 100% | 100% | |
| 3 | human | 5015 | OTX2 | orthodenticle homeobox 2 | NM_001270523.2 | 97.3% | 97.3% | 96_97ins24 |
| 4 | human | 5015 | OTX2 | orthodenticle homeobox 2 | NM_001270524.2 | 97.3% | 97.3% | 96_97ins24 |
| 5 | human | 5015 | OTX2 | orthodenticle homeobox 2 | NM_172337.3 | 97.3% | 97.3% | 96_97ins24 |
| 6 | human | 5015 | OTX2 | orthodenticle homeobox 2 | NR_073036.2 | 24.3% | 1_211del;307_308ins176;927_2759del | |
| 7 | human | 5015 | OTX2 | orthodenticle homeobox 2 | NR_073034.2 | 23.7% | 1_287del;383_384ins176;1003_2835del | |
| 8 | mouse | 18424 | Otx2 | orthodenticle homeobox 2 | NM_001286481.1 | 94.8% | 99.3% | (many diffs) |
| 9 | mouse | 18424 | Otx2 | orthodenticle homeobox 2 | XM_011244988.2 | 94.8% | 99.3% | (many diffs) |
| 10 | mouse | 18424 | Otx2 | orthodenticle homeobox 2 | XM_017315913.1 | 94.8% | 99.3% | (many diffs) |
| 11 | mouse | 18424 | Otx2 | orthodenticle homeobox 2 | XM_017315914.1 | 94.8% | 99.3% | (many diffs) |
| 12 | mouse | 18424 | Otx2 | orthodenticle homeobox 2 | XM_017315915.1 | 94.8% | 99.3% | (many diffs) |
| 13 | mouse | 18424 | Otx2 | orthodenticle homeobox 2 | XM_017315916.1 | 94.8% | 99.3% | (many diffs) |
| 14 | mouse | 18424 | Otx2 | orthodenticle homeobox 2 | NM_001286482.1 | 92.2% | 96.6% | (many diffs) |
| 15 | mouse | 18424 | Otx2 | orthodenticle homeobox 2 | NM_001286483.1 | 92.2% | 96.6% | (many diffs) |
| 16 | mouse | 18424 | Otx2 | orthodenticle homeobox 2 | NM_144841.4 | 92.2% | 96.6% | (many diffs) |
| 17 | mouse | 18424 | Otx2 | orthodenticle homeobox 2 | XM_011244989.2 | 92.2% | 96.6% | (many diffs) |
| 18 | mouse | 18424 | Otx2 | orthodenticle homeobox 2 | XM_017315917.1 | 92.2% | 96.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 957
- ORF length:
- 891
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat gtcttatctt aagcaaccgc cttacgcagt caatgggctg agtctgacca 121 cttcgggtat ggacttgctg cacccctccg tgggctaccc ggggccctgg gcttcttgtc 181 ccgcagccac cccccggaaa cagcgccggg agaggacgac gttcactcgg gcgcagctag 241 atgtgctgga agcactgttt gccaagaccc ggtacccaga catcttcatg cgagaggagg 301 tggcactgaa aatcaacttg cccgagtcga gggtgcaggt atggtttaag aatcgaagag 361 ctaagtgccg ccaacaacag caacaacagc agaatggagg tcaaaacaaa gtgagacctg 421 ccaaaaagaa gacatctcca gctcgggaag tgagttcaga gagtggaaca agtggccaat 481 tcactccccc ctctagcacc TCAGTCCCGA CCATTGCCAG CAGCAGTGCT CCTGTGTCTA 541 TCTGGAGCCC AGCTTCCATC TCCCCACTGT CAGATCCCTT GTCCACCTCC TCTTCCTGCA 601 TGCAGAGGTC CTATCCCATG ACCTATACTC AGGCTTCAGG TTATAGTCAA GGATATGCTG 661 GCTCAACTTC CTACTTTGGG GGCATGGACT GTGGATCATA TTTGACCCCT ATGCATCACC 721 AGCTTCCCGG ACCAGGGGCC ACACTCAGTC CCATGGGTAC CAATGCAGTC ACCAGCCATC 781 TCAATCAGTC CCCAGCTTCT CTTTCCACCC AGGGATATGG AGCTTCAAGC TTGGGTTTTA 841 ACTCAACCAC TGATTGCTTG GATTATAAGG ACCAAACTGC CTCCTGGAAG CTTAACTTCA 901 ATGCTGACTG CTTGGATTAT AAAGATCAGA CATCCTCGTG GAAATTCCAG GTTTTGTGCC 961 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1021 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1081 ATATATCTTG TGGAAAGGAC GATCCGCACG CTCGAAAAAA CGTCGTACGC GTTAAGTCga 1141 caatcaacct ctggattaca aaatttgtga aagatt