Transcript: Human NM_001288823.1

Homo sapiens olfactomedin 3 (OLFM3), transcript variant 3, mRNA.

Source:
NCBI, updated 2019-08-05
Taxon:
Homo sapiens (human)
Gene:
OLFM3 (118427)
Length:
3538
CDS:
705..1856

Additional Resources:

NCBI RefSeq record:
NM_001288823.1
NBCI Gene record:
OLFM3 (118427)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001288823.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000187076 CCCTTCCATAACCAATACTTT pLKO.1 1716 CDS 100% 5.625 7.875 N OLFM3 n/a
2 TRCN0000186737 GCCAAGGTGTATTATTCCTAT pLKO.1 1659 CDS 100% 4.950 6.930 N OLFM3 n/a
3 TRCN0000202922 CCATAACCAATACTTTCACAT pLKO.1 1721 CDS 100% 4.950 3.960 N Olfm3 n/a
4 TRCN0000185699 GCAGTATGTATTTCTACCATT pLKO.1 2419 3UTR 100% 4.950 3.465 N OLFM3 n/a
5 TRCN0000189157 GCTGTCTTTGTTTGCCGGATT pLKO.1 454 5UTR 100% 4.050 2.835 N OLFM3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001288823.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_09451 pDONR223 100% 83.5% 83.4% None 0_1ins225;1076A>G n/a
2 ccsbBroad304_09451 pLX_304 0% 83.5% 83.4% V5 0_1ins225;1076A>G n/a
3 TRCN0000468203 CTACGGACGCCAGGAGGAATACTG pLX_317 22.1% 83.5% 83.4% V5 0_1ins225;1076A>G n/a
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