Construct: ORF TRCN0000468203
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017226.1_s317c1
- Derived from:
- ccsbBroadEn_09451
- DNA Barcode:
- CTACGGACGCCAGGAGGAATACTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- OLFM3 (118427)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468203
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 118427 | OLFM3 | olfactomedin 3 | NM_058170.3 | 99.7% | 99.5% | 141C>T;202C>A;1301A>G |
| 2 | human | 118427 | OLFM3 | olfactomedin 3 | NM_001288821.1 | 92.6% | 92% | (many diffs) |
| 3 | human | 118427 | OLFM3 | olfactomedin 3 | NM_001288823.1 | 83.5% | 83.4% | 0_1ins225;1076A>G |
| 4 | human | 118427 | OLFM3 | olfactomedin 3 | XM_017000240.1 | 83.5% | 83.4% | 0_1ins225;1076A>G |
| 5 | human | 118427 | OLFM3 | olfactomedin 3 | NR_110212.1 | 33.7% | (many diffs) | |
| 6 | human | 118427 | OLFM3 | olfactomedin 3 | NR_110211.1 | 32.5% | (many diffs) | |
| 7 | human | 118427 | OLFM3 | olfactomedin 3 | NR_110210.1 | 32% | (many diffs) | |
| 8 | mouse | 229759 | Olfm3 | olfactomedin 3 | NM_153458.3 | 90.9% | 98% | (many diffs) |
| 9 | mouse | 229759 | Olfm3 | olfactomedin 3 | NM_001286750.1 | 88.3% | 93.3% | (many diffs) |
| 10 | mouse | 229759 | Olfm3 | olfactomedin 3 | NM_153157.3 | 84.9% | 91.4% | (many diffs) |
| 11 | mouse | 229759 | Olfm3 | olfactomedin 3 | XM_006501418.1 | 76.2% | 82.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1440
- ORF length:
- 1374
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca ggcgacgtcc aaccttctca acctcctgct gctgtctttg tttgccggat 121 tagatccttc caagactcag attagtccta aagaagggtg gcaggtgtac agctcagctc 181 aggatcctga tgggcggtgc atttgtacag ttgttgctcc agaacaaaac ctgtgttccc 241 gggatgccaa aagcaggcaa cttcgcaaac tactggaaaa ggttcagaac atgtcccagt 301 ctattgaagt cttaaacttg agaactcaga gagatttcca atatgtttta aaaatggaaa 361 cccaaatgaa agggctgaag gcaaaatttc ggcagattga agatgatcga aagacactta 421 tgaccaagca ttttcaggag ttgaaagaga aaatggacga gctcctgcct ttgatccccg 481 tgctggaaca gtacaaaaca gatgctaagt taatcaccca gttcaaggag gaaataagga 541 atctgtctgc tgtcctcact ggtattcagg aggaaattgg tgcctatgac tacgaggaac 601 tacaccaaag agtgctgagc ttggaaacaa gacttcgtga ctgcatgaaa aagctaacat 661 gtggcaaact gatgaaaatc acaggcccag ttacagtcaa gacatctgga acccgatttg 721 gtgcttggat gacagaccct ttagcatctg agaaaaacaa cagagtctgg tacatggaca 781 gttatactaa caataaaatt gttcgtgaat acaaatcaat tgcagacttt gtcagtgggg 841 ctgaatcaag gacatacaac cttcctttca agtgggcagg aactaaccat gttgtctaca 901 atggctcact ctattttaac aagtatcaga gtaatatcat catcaaatac agctttgata 961 tggggagagt gcttgcccaa cgaagcctgg agtatgctgg ttttcataat gtttacccct 1021 acacatgggg tggattctct gacatcgacc taatggctga tgaaatcggg ctgtgggctg 1081 tgtatgcaac taaccagaat gcaggcaata ttgtcatcag ccaacttaac caagatacct 1141 tGGAGGTGAT GAAGAGCTGG AGCACTGGCT ACCCCAAGAG AAGTGCAGGG GAATCTTTCA 1201 TGATCTGTGG GACACTGTAT GTCACCAACT CCCACTTAAC TGGAGCCAAG GTGTATTATT 1261 CCTATTCCAC CAAAACCTCC ACATATGAGT ACACAGACAT TCCCTTCCAT AACCAATACT 1321 TTCACATATC CATGCTTGAC TACAATGCAA GAGATCGAGC TCTCTGTGCC TGGAACAATG 1381 GCCACCAGGT GCTGTTCAAT GTCACCCTTT TCCATATCAT CAAGACAGAG GATGACACAT 1441 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1501 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1561 TTTATATATC TTGTGGAAAG GACGACTACG GACGCCAGGA GGAATACTGA CGCGTTAAGT 1621 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt