Transcript: Mouse NM_001290502.1

Mus musculus tetratricopeptide repeat domain 14 (Ttc14), transcript variant 4, mRNA.

Source:
NCBI, updated 2017-06-04
Taxon:
Mus musculus (mouse)
Gene:
Ttc14 (67120)
Length:
9572
CDS:
344..1651

Additional Resources:

NCBI RefSeq record:
NM_001290502.1
NBCI Gene record:
Ttc14 (67120)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001290502.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000094016 CGAGATATTGAACGTGGTGAT pLKO.1 692 CDS 100% 4.050 5.670 N Ttc14 n/a
2 TRCN0000094015 CGTGAGAGATATTTCGCACTT pLKO.1 787 CDS 100% 4.050 5.670 N Ttc14 n/a
3 TRCN0000094017 GCCCAACTCATAGGAATGCAA pLKO.1 1443 CDS 100% 3.000 4.200 N Ttc14 n/a
4 TRCN0000094014 GCATTATAGTACCACTGCTTT pLKO.1 1752 3UTR 100% 4.950 3.960 N Ttc14 n/a
5 TRCN0000094018 GAGATATTGAACGTGGTGATA pLKO.1 693 CDS 100% 4.950 3.465 N Ttc14 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001290502.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_05053 pDONR223 100% 48.2% 46.5% None (many diffs) n/a
2 ccsbBroad304_05053 pLX_304 0% 48.2% 46.5% V5 (many diffs) n/a
3 TRCN0000467798 TCTAAGTAAAGCATAAAATGGTCC pLX_317 18.6% 48.2% 46.5% V5 (many diffs) n/a
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