Transcript: Mouse NM_001290522.1

Mus musculus WD repeat domain 31 (Wdr31), transcript variant 3, mRNA.

Source:
NCBI, updated 2017-06-04
Taxon:
Mus musculus (mouse)
Gene:
Wdr31 (71354)
Length:
2447
CDS:
202..1302

Additional Resources:

NCBI RefSeq record:
NM_001290522.1
NBCI Gene record:
Wdr31 (71354)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001290522.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000313720 AGCGGGAAATCACGAAGATAG pLKO_005 506 CDS 100% 10.800 15.120 N Wdr31 n/a
2 TRCN0000113281 GAGCGGGAAATCACGAAGATA pLKO.1 505 CDS 100% 5.625 7.875 N Wdr31 n/a
3 TRCN0000113280 CCTCCTTGTTATGCGCAAGTT pLKO.1 1208 CDS 100% 4.950 6.930 N Wdr31 n/a
4 TRCN0000317310 CCTCCTTGTTATGCGCAAGTT pLKO_005 1208 CDS 100% 4.950 6.930 N Wdr31 n/a
5 TRCN0000113282 ACGGCCTTGATGCCTATGATT pLKO.1 1069 CDS 100% 5.625 4.500 N Wdr31 n/a
6 TRCN0000349986 GACAGAATGTGTGAGTATAAG pLKO_005 1003 CDS 100% 13.200 9.240 N Wdr31 n/a
7 TRCN0000313721 TGGATGGACACACGTGTATTT pLKO_005 917 CDS 100% 13.200 9.240 N Wdr31 n/a
8 TRCN0000113283 CTCTGAACTCAGACCTTTGTA pLKO.1 407 CDS 100% 5.625 3.938 N Wdr31 n/a
9 TRCN0000317239 CTCTGAACTCAGACCTTTGTA pLKO_005 407 CDS 100% 5.625 3.938 N Wdr31 n/a
10 TRCN0000113284 CCACTCTGAACTCAGACCTTT pLKO.1 404 CDS 100% 4.950 3.465 N Wdr31 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001290522.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04673 pDONR223 100% 85.1% 83% None (many diffs) n/a
2 ccsbBroad304_04673 pLX_304 0% 85.1% 83% V5 (many diffs) n/a
3 TRCN0000470624 GCAATCACTTGGACAAACTCACCC pLX_317 41.8% 85.1% 83% V5 (many diffs) n/a
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