Construct: ORF TRCN0000470624
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001510.1_s317c1
- Derived from:
- ccsbBroadEn_04673
- DNA Barcode:
- GCAATCACTTGGACAAACTCACCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- WDR31 (114987)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470624
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 114987 | WDR31 | WD repeat domain 31 | NM_145241.5 | 100% | 100% | |
| 2 | human | 114987 | WDR31 | WD repeat domain 31 | NM_001012361.4 | 99.7% | 99.7% | 116_118delGCA |
| 3 | human | 114987 | WDR31 | WD repeat domain 31 | XM_011518194.2 | 92.9% | 92.9% | 116_118delGCA;246_247ins75 |
| 4 | human | 114987 | WDR31 | WD repeat domain 31 | NM_001006615.3 | 66.1% | 66.1% | 0_1ins372 |
| 5 | human | 114987 | WDR31 | WD repeat domain 31 | NR_134677.2 | 20.9% | (many diffs) | |
| 6 | mouse | 71354 | Wdr31 | WD repeat domain 31 | NM_001290522.1 | 85.1% | 83% | (many diffs) |
| 7 | mouse | 71354 | Wdr31 | WD repeat domain 31 | NM_001290521.1 | 84.9% | 82.8% | (many diffs) |
| 8 | mouse | 71354 | Wdr31 | WD repeat domain 31 | NM_023597.2 | 84.9% | 82.8% | (many diffs) |
| 9 | mouse | 71354 | Wdr31 | WD repeat domain 31 | XM_006538254.2 | 84.9% | 82.8% | (many diffs) |
| 10 | mouse | 71354 | Wdr31 | WD repeat domain 31 | XR_390342.3 | 43.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1164
- ORF length:
- 1098
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct gctactcagg tgccaactga aacaagctcc tccacagaag gtttcgttta 121 ggttttgtgt cgtgatgggg aaacagcaaa gcaaactcaa acacagcact tataaatacg 181 ggcctgatga aattatagaa gagagaattc aaactaaagc ttttcaagag tatagcccag 241 ctcacatgga taccgtctct gtcgtggctg ctttgaactc agacctttgt gtctctggag 301 ggaaagataa gacagttgtg gcctataatt ggaaaactgg aaatgtggtg aaaaggttca 361 aaggacatga acatgagatc accaaggtag cctgtattcc caaatccagc cagttcttca 421 gtgcctctcg tgacaggatg gtcatgatgt gggacttgca cggttcctca caaccaaggc 481 agcaattgtg tggccatgcc atggtggtca ccggattggc tgtgagtcca gactcatcac 541 agctgtgcac tggctctcgg gacaacaccc tgcttctgtg ggatgtggtg acaggacaga 601 gtgtggaaag agcatctgtc tccaggaacg tggtcactca cctgtgctgg gtccccagag 661 aaccatacat actacagacc tctgaagata aaaccctcag attatgGGAC AGTCGGGGGC 721 TGCAGGTAGC TCATATGTTT CCTGCAAAGC AGCACATTCA GACCTACTGT GAAGTCAGTG 781 TGGATGGACA CAAGTGTATC TCCTGCAGCA ATGGCTTTGG AGGAGAAGGC TGTGAAGCCA 841 CGTTGTGGGA CCTAAGACAG ACTCGAAACA GAATATGTGA GTATAAGGGG CATTTCCAGA 901 CTGTCGCATC CTGCGTCTTT CTACCAAGAG CATTGGCCTT GATGCCTTTA ATTGCTACCT 961 CATCACATGA TTGCAAGGTG AAGATTTGGA ACCAAGATAC TGGAGCCTGC CTTTTCACCT 1021 TGTCTCTGGA TGGATCAGGA CCCTTGACTT CTCTGGCTGT TGGTGACGCC ATCTCCTTAT 1081 TGTGTGCAAG TTTTAACAGA GGAATTCACT TACTCAGAAT GGACCACAGC CAAGGGCTGG 1141 AACTGCAGGA AGTGGCAGCA TTCTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1201 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1261 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAG CAATCACTTG 1321 GACAAACTCA CCCACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1381 att