Construct: ORF TRCN0000467494
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017383.1_s317c1
- Derived from:
- ccsbBroadEn_13290
- DNA Barcode:
- GTTCTTGGTGCAGCTCGATGCTAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RASGEF1B (153020)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467494
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 153020 | RASGEF1B | RasGEF domain family member 1B | NM_001300735.2 | 100% | 100% | |
| 2 | human | 153020 | RASGEF1B | RasGEF domain family member 1B | NM_152545.3 | 99.7% | 99.7% | 437_439delAGC |
| 3 | human | 153020 | RASGEF1B | RasGEF domain family member 1B | XM_017007813.1 | 99.7% | 99.7% | 437_439delAGC |
| 4 | human | 153020 | RASGEF1B | RasGEF domain family member 1B | NM_001300736.2 | 91.3% | 91.3% | 177_178ins123 |
| 5 | human | 153020 | RASGEF1B | RasGEF domain family member 1B | XM_017007812.1 | 91.1% | 91.1% | 177_178ins123;314_316delAGC |
| 6 | mouse | 320292 | Rasgef1b | RasGEF domain family, membe... | NM_181318.4 | 87.2% | 97.4% | (many diffs) |
| 7 | mouse | 320292 | Rasgef1b | RasGEF domain family, membe... | NM_145839.2 | 87.1% | 97.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1485
- ORF length:
- 1416
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcctcagact cctccctttt cagcaatgtt tgacagcagt ggttacaatc 121 gaaacctcta tcagtctgca gaggacagct gtggagggtt gtattaccat gacaacaacc 181 tcctctctgg atccctggaa gcactcatcc agcacttagt acctaatgtg gattactatc 241 cagatagaac atacatattt accttcctac tcagttctcg gttatttatg catccgtatg 301 agctaatggc caaagtttgc cacttatgtg ttgagcacca gagactaagt gatcctgata 361 gtgataagaa ccagatgaga aaaattgcac ccaaaatcct tcaactcctc acggaatgga 421 cggaaacatt tccctatgat tttcgggatg aaagaatgat gagaaactta aaagatctgg 481 ctcaccgaat agccagtggc gaagagacat acagaaagaa tgtccagcaa atgatgcagt 541 gtctgatccg caagcttgct gcgctcagcc agtacgaaga agtcctggca aaaatcagct 601 ccacatccac agatcggctc acagttctca agaccaagcc acagtctata caaagggata 661 tcattactgt ctgcaacgac ccttacacgt tggcccagca gctgactcat atagagctgg 721 agaggctcaa ttatattggg ccagaagaat ttgttcaggc gttcgtgcag aaggaccctt 781 tggataatga caagagttgc tacagtgaac ggaagaaaac acgaaactta gaagcttacg 841 tggaatggtt taatcgcctc agctacttgg ttgctacaga aatctgtatg cctgttaaga 901 aaaaacaccg agcaagaatg attgagtatt tcattgacgt agctcgggag tgttttaaca 961 ttggcaactt caactccttg atggcgataa tctctggtat gaatatgagc ccagtctctc 1021 gactaaaaaa aacttgggcc aaagtgaaga ctgcaaAATT TGACATTCTT GAGCATCAGA 1081 TGGACCCTTC AAGCAATTTC TATAATTATC GAACAGCTCT TCGTGGGGCA GCACAAAGGT 1141 CTTTAACTGC TCATAGTAGT AGAGAAAAGA TTGTGATACC ATTCTTCAGT CTCTTAATCA 1201 AAGATATTTA TTTCCTCAAT GAGGGTTGTG CCAACCGCCT TCCCAATGGC CATGTCAATT 1261 TTGAGAAATT TTGGGAACTG GCCAAACAAG TGAGTGAATT TATGACATGG AAACAAGTGG 1321 AGTGTCCATT TGAGAGGGAC CGGAAGATCT TGCAGTATCT GCTCACAGTA CCAGTCTTCA 1381 GTGAAGATGC TCTCTACTTG GCTTCTTATG AGAGTGAAGG ACCTGAAAAT CATATAGAGA 1441 AAGACAGATG GAAGTCTTTA AGGTCGAGCC TCTTAGGCAG AGTTTTGCCA ACTTTCTTGT 1501 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1561 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1621 GAAAGGACGA GTTCTTGGTG CAGCTCGATG CTATACGCGT TAAGTCgaca atcaacctct 1681 ggattacaaa atttgtgaaa gatt