Transcript: Human NM_001308274.1

Homo sapiens ring finger protein 32 (RNF32), transcript variant 5, mRNA.

Source:
NCBI, updated 2019-08-03
Taxon:
Homo sapiens (human)
Gene:
RNF32 (140545)
Length:
2265
CDS:
78..785

Additional Resources:

NCBI RefSeq record:
NM_001308274.1
NBCI Gene record:
RNF32 (140545)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001308274.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000434613 CATGATCTTCAACTTCGAAAT pLKO_005 156 CDS 100% 10.800 7.560 N RNF32 n/a
2 TRCN0000034108 GAGAGGATGTGTTGTTAGAAA pLKO.1 668 CDS 100% 5.625 3.938 N RNF32 n/a
3 TRCN0000034107 GTAGAAAGAACCAGTATCAAA pLKO.1 580 CDS 100% 5.625 3.938 N RNF32 n/a
4 TRCN0000034105 CCCAAACTAGAAGACTCAGAA pLKO.1 288 CDS 100% 4.950 3.465 N RNF32 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001308274.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13201 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_13201 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000469877 TTACACTTCTAATATACCATCCTT pLX_317 57.5% 100% 100% V5 n/a
4 TRCN0000487699 GAATAAAGAGTCCCGAAAGTGAGT pLX_317 34.3% 100% 100% V5 (not translated due to prior stop codon) n/a
5 ccsbBroadEn_14386 pDONR223 100% 89.6% 87.8% None (many diffs) n/a
6 ccsbBroad304_14386 pLX_304 0% 89.6% 87.8% V5 (many diffs) n/a
7 TRCN0000473770 CTGTGGCTAACTTGGAGCGGCTAC pLX_317 62.4% 89.6% 87.8% V5 (many diffs) n/a
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