Construct: ORF TRCN0000469877
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000818.1_s317c1
- Derived from:
- ccsbBroadEn_13201
- DNA Barcode:
- TTACACTTCTAATATACCATCCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RNF32 (140545)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469877
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 140545 | RNF32 | ring finger protein 32 | NM_001308274.1 | 100% | 100% | |
| 2 | human | 140545 | RNF32 | ring finger protein 32 | XM_017011755.1 | 100% | 100% | |
| 3 | human | 140545 | RNF32 | ring finger protein 32 | XM_024446666.1 | 100% | 100% | |
| 4 | human | 140545 | RNF32 | ring finger protein 32 | NM_001308273.2 | 74.3% | 73.5% | (many diffs) |
| 5 | human | 140545 | RNF32 | ring finger protein 32 | XM_017011754.1 | 74.3% | 73.5% | (many diffs) |
| 6 | human | 140545 | RNF32 | ring finger protein 32 | XR_927369.2 | 66.6% | 1_230del;647_663del;953_1057del | |
| 7 | human | 140545 | RNF32 | ring finger protein 32 | XM_017011753.1 | 63.9% | 63.1% | (many diffs) |
| 8 | human | 140545 | RNF32 | ring finger protein 32 | NM_001184996.1 | 63.7% | 62.9% | (many diffs) |
| 9 | human | 140545 | RNF32 | ring finger protein 32 | NM_001184997.1 | 63.7% | 62.9% | (many diffs) |
| 10 | human | 140545 | RNF32 | ring finger protein 32 | NM_030936.3 | 63.7% | 62.9% | (many diffs) |
| 11 | human | 140545 | RNF32 | ring finger protein 32 | XM_005249522.5 | 63.7% | 62.9% | (many diffs) |
| 12 | human | 140545 | RNF32 | ring finger protein 32 | XM_011515804.3 | 63.7% | 62.9% | (many diffs) |
| 13 | human | 140545 | RNF32 | ring finger protein 32 | XM_011515805.3 | 63.7% | 62.9% | (many diffs) |
| 14 | human | 140545 | RNF32 | ring finger protein 32 | XM_011515806.3 | 63.7% | 62.9% | (many diffs) |
| 15 | human | 140545 | RNF32 | ring finger protein 32 | XM_011515807.3 | 63.7% | 62.9% | (many diffs) |
| 16 | human | 140545 | RNF32 | ring finger protein 32 | XM_011515808.3 | 63.7% | 62.9% | (many diffs) |
| 17 | human | 140545 | RNF32 | ring finger protein 32 | XM_011515809.3 | 63.7% | 62.9% | (many diffs) |
| 18 | human | 140545 | RNF32 | ring finger protein 32 | XM_011515810.3 | 63.7% | 62.9% | (many diffs) |
| 19 | human | 140545 | RNF32 | ring finger protein 32 | XM_011515811.3 | 63.7% | 62.9% | (many diffs) |
| 20 | human | 140545 | RNF32 | ring finger protein 32 | XM_011515812.3 | 63.7% | 62.9% | (many diffs) |
| 21 | human | 140545 | RNF32 | ring finger protein 32 | XR_428168.3 | 62.1% | 1_234del;684_685ins56;884_988del | |
| 22 | human | 140545 | RNF32 | ring finger protein 32 | XM_011515813.2 | 57.7% | 55.7% | (many diffs) |
| 23 | human | 140545 | RNF32 | ring finger protein 32 | NR_131778.1 | 39.4% | (many diffs) | |
| 24 | human | 140545 | RNF32 | ring finger protein 32 | XR_001744553.1 | 29.4% | (many diffs) | |
| 25 | human | 140545 | RNF32 | ring finger protein 32 | XR_001744552.1 | 28.2% | (many diffs) | |
| 26 | human | 140545 | RNF32 | ring finger protein 32 | XR_927367.3 | 26.1% | (many diffs) | |
| 27 | mouse | 56874 | Rnf32 | ring finger protein 32 | XM_017321035.1 | 42.8% | 43.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 771
- ORF length:
- 705
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt aaaaaataag ggtcactcat ctaagaaaga taacttggca gtcaatgcag 121 ttgctttaca agatcacatt ttacatgatc ttcaacttcg aaatctttca gttgcagatc 181 attctaagac acaagtacaa aagaaagaga acaaatctct aaaaagagat acaaaggcaa 241 taatagatac tggacttaaa aaaactacac agtgccccaa actagaagac tcagaaaaag 301 aatatgttct tgatcccaaa ccgccgccgt tgactttggc acagaagttg ggcctcattg 361 ggccTCCACC ACCTCCACTG TCATCAGATG AATGGGAGAA GGTGAAACAG CGCTCTCTCC 421 TGCAAGGGGA CTCCGTGCAA CCATGCCCCA TCTGTAAAGA AGAATTCGAG CTTCGTCCTC 481 AGGTGCTGCT TTCATGCTCC CATGTGTTCC ACAAAGCATG TCTTCAGGCT TTTGAAAAGT 541 TCACAAATAA GAAAACCTGT CCTCTCTGTA GAAAGAACCA GTATCAAACC CGAGTGATAC 601 ACGATGGGGC CCGCCTGTTC AGAATCAAGT GTGTGACCAG AATCCAAGCC TACTGGAGAG 661 GATGTGTTGT TAGAAAGTGG TACAGAAACC TGAGGAAAAC AGTACCTCCC ACAGATGCCA 721 AGTTAAGAAA AAAATTCTTT GAAAAAAAGA CACAAGACTG GAAACCAGCT TACCCAACTT 781 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 841 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 901 CTTGTGGAAA GGACGATTAC ACTTCTAATA TACCATCCTT ACGCGTTAAG TCgacaatca 961 acctctggat tacaaaattt gtgaaagatt