Construct: ORF TRCN0000487699
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021319.1_s317c1
- DNA Barcode:
- GAATAAAGAGTCCCGAAAGTGAGT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- RNF32 (140545)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487699
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 140545 | RNF32 | ring finger protein 32 | NM_001308274.1 | 100% | 100% | |
| 2 | human | 140545 | RNF32 | ring finger protein 32 | XM_017011755.1 | 100% | 100% | |
| 3 | human | 140545 | RNF32 | ring finger protein 32 | XM_024446666.1 | 100% | 100% | |
| 4 | human | 140545 | RNF32 | ring finger protein 32 | NM_001308273.2 | 74.3% | 73.5% | (many diffs) |
| 5 | human | 140545 | RNF32 | ring finger protein 32 | XM_017011754.1 | 74.3% | 73.5% | (many diffs) |
| 6 | human | 140545 | RNF32 | ring finger protein 32 | XR_927369.2 | 66.6% | 1_230del;647_663del;953_1057del | |
| 7 | human | 140545 | RNF32 | ring finger protein 32 | XM_017011753.1 | 63.9% | 63.1% | (many diffs) |
| 8 | human | 140545 | RNF32 | ring finger protein 32 | NM_001184996.1 | 63.7% | 62.9% | (many diffs) |
| 9 | human | 140545 | RNF32 | ring finger protein 32 | NM_001184997.1 | 63.7% | 62.9% | (many diffs) |
| 10 | human | 140545 | RNF32 | ring finger protein 32 | NM_030936.3 | 63.7% | 62.9% | (many diffs) |
| 11 | human | 140545 | RNF32 | ring finger protein 32 | XM_005249522.5 | 63.7% | 62.9% | (many diffs) |
| 12 | human | 140545 | RNF32 | ring finger protein 32 | XM_011515804.3 | 63.7% | 62.9% | (many diffs) |
| 13 | human | 140545 | RNF32 | ring finger protein 32 | XM_011515805.3 | 63.7% | 62.9% | (many diffs) |
| 14 | human | 140545 | RNF32 | ring finger protein 32 | XM_011515806.3 | 63.7% | 62.9% | (many diffs) |
| 15 | human | 140545 | RNF32 | ring finger protein 32 | XM_011515807.3 | 63.7% | 62.9% | (many diffs) |
| 16 | human | 140545 | RNF32 | ring finger protein 32 | XM_011515808.3 | 63.7% | 62.9% | (many diffs) |
| 17 | human | 140545 | RNF32 | ring finger protein 32 | XM_011515809.3 | 63.7% | 62.9% | (many diffs) |
| 18 | human | 140545 | RNF32 | ring finger protein 32 | XM_011515810.3 | 63.7% | 62.9% | (many diffs) |
| 19 | human | 140545 | RNF32 | ring finger protein 32 | XM_011515811.3 | 63.7% | 62.9% | (many diffs) |
| 20 | human | 140545 | RNF32 | ring finger protein 32 | XM_011515812.3 | 63.7% | 62.9% | (many diffs) |
| 21 | human | 140545 | RNF32 | ring finger protein 32 | XR_428168.3 | 62.1% | 1_234del;684_685ins56;884_988del | |
| 22 | human | 140545 | RNF32 | ring finger protein 32 | XM_011515813.2 | 57.7% | 55.7% | (many diffs) |
| 23 | human | 140545 | RNF32 | ring finger protein 32 | NR_131778.1 | 39.4% | (many diffs) | |
| 24 | human | 140545 | RNF32 | ring finger protein 32 | XR_001744553.1 | 29.4% | (many diffs) | |
| 25 | human | 140545 | RNF32 | ring finger protein 32 | XR_001744552.1 | 28.2% | (many diffs) | |
| 26 | human | 140545 | RNF32 | ring finger protein 32 | XR_927367.3 | 26.1% | (many diffs) | |
| 27 | mouse | 56874 | Rnf32 | ring finger protein 32 | XM_017321035.1 | 42.8% | 43.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 777
- ORF length:
- 705
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgttaaaa aataagggtc actcatctaa gaaagataac ttggcagtca 121 atgcagttgc tttacaagat cacattttac atgatcttca acttcgaaat ctttcagttg 181 cagatcattc taagacacaa gtacaaaaga aagagaacaa atctctaaaa agagatacaa 241 aggcaataat agatactgga cttaaaaaaa ctacacagtg ccccaaacta gaagactcag 301 aaaaagaata tgttcttgat cccaaaccgc cgccgttgac tttggcacag aagttgggcc 361 tcattgggcc tccaccacct ccactgtcat cagatgaatg ggagaaggtg aaacagcgct 421 ctctcctgca aggggactcc gtgcaaccat gccccatctg taaagaagaa ttcgagcttc 481 gtcctcaggt gctgctttca tgctcccatg tgttccacaa agcatgtctt caggctttTG 541 AAAAGTTCAC AAATAAGAAA ACCTGTCCTC TCTGTAGAAA GAACCAGTAT CAAACCCGAG 601 TGATACACGA TGGGGCCCGC CTGTTCAGAA TCAAGTGTGT GACCAGAATC CAAGCCTACT 661 GGAGAGGATG TGTTGTTAGA AAGTGGTACA GAAACCTGAG GAAAACAGTA CCTCCCACAG 721 ATGCCAAGTT AAGAAAAAAA TTCTTTGAAA AAAAGACACA AGACTGGAAA CCAGCTTAGA 781 ACCCAGCTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 841 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 901 TTTATATATC TTGTGGAAAG GACGAGAATA AAGAGTCCCG AAAGTGAGTA CGCGTTAAGT 961 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt