Transcript: Human NM_001321136.2

Homo sapiens proline-serine-threonine phosphatase interacting protein 1 (PSTPIP1), transcript variant 3, mRNA.

Source:
NCBI, updated 2019-09-16
Taxon:
Homo sapiens (human)
Gene:
PSTPIP1 (9051)
Length:
2217
CDS:
695..1918

Additional Resources:

NCBI RefSeq record:
NM_001321136.2
NBCI Gene record:
PSTPIP1 (9051)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001321136.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000367620 CGGTGCCCTACCAGAACTATT pLKO_005 1509 CDS 100% 13.200 18.480 N PSTPIP1 n/a
2 TRCN0000002989 CTACGATTATACAGCGCAGAA pLKO.1 1768 CDS 100% 4.050 5.670 N PSTPIP1 n/a
3 TRCN0000356123 ATGGAGTCCAAGAAGACATAC pLKO_005 1079 CDS 100% 10.800 7.560 N PSTPIP1 n/a
4 TRCN0000367595 GACTCCTTGAAGCAGCAAATG pLKO_005 899 CDS 100% 10.800 7.560 N PSTPIP1 n/a
5 TRCN0000002987 GTGGAGAAGAGTCAGAACAAA pLKO.1 1175 CDS 100% 5.625 3.938 N PSTPIP1 n/a
6 TRCN0000367548 AGACGCCGACATCGACAGTTT pLKO_005 1450 CDS 100% 4.950 3.465 N PSTPIP1 n/a
7 TRCN0000010750 TGCAAAGACATGGAGGAGCTA pLKO.1 773 CDS 100% 2.640 1.848 N PSTPIP1 n/a
8 TRCN0000002990 CGACAGTTTCATCCAGGCCAA pLKO.1 1462 CDS 100% 2.160 1.512 N PSTPIP1 n/a
9 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 489 5UTR 100% 5.625 2.813 Y KLHL30 n/a
10 TRCN0000002988 TAAAGGAGTGCGTTCTGTTCA pLKO.1 2053 3UTR 100% 4.950 3.465 N PSTPIP1 n/a
11 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 489 5UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001321136.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07348 pDONR223 100% 97.8% 97.1% None 3_4insATACCCCAGCTGCA;8_9insAAGATGCCTTTTG n/a
2 ccsbBroad304_07348 pLX_304 0% 97.8% 97.1% V5 3_4insATACCCCAGCTGCA;8_9insAAGATGCCTTTTG n/a
3 TRCN0000467183 ACACGATCCGCGTCCTTACAATAC pLX_317 13.2% 97.8% 97.1% V5 3_4insATACCCCAGCTGCA;8_9insAAGATGCCTTTTG n/a
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