Transcript: Human NM_001347955.2

Homo sapiens rabphilin 3A (RPH3A), transcript variant 6, mRNA.

Source:
NCBI, updated 2019-09-16
Taxon:
Homo sapiens (human)
Gene:
RPH3A (22895)
Length:
4501
CDS:
517..2400

Additional Resources:

NCBI RefSeq record:
NM_001347955.2
NBCI Gene record:
RPH3A (22895)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001347955.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000419241 CATCGGCAAGTCCAATGATTA pLKO_005 2244 CDS 100% 13.200 18.480 N RPH3A n/a
2 TRCN0000220581 CCCGAATTCAATGAGGAGTTT pLKO.1 2158 CDS 100% 0.000 0.000 N RPH3A n/a
3 TRCN0000220582 CCCACAGCCTATGCCTATAAA pLKO.1 813 CDS 100% 15.000 10.500 N RPH3A n/a
4 TRCN0000220580 CCTCTCAGTTTGGGTAAATTA pLKO.1 2684 3UTR 100% 15.000 10.500 N RPH3A n/a
5 TRCN0000419452 TTTGGCCACAATGAATTTATT pLKO_005 1777 CDS 100% 15.000 10.500 N RPH3A n/a
6 TRCN0000419397 CCTGCAGTGCACCATCATTAA pLKO_005 1539 CDS 100% 13.200 9.240 N RPH3A n/a
7 TRCN0000435461 GAACCACGTGTCAAGTGATTA pLKO_005 2379 CDS 100% 13.200 9.240 N RPH3A n/a
8 TRCN0000436342 AGCGAGTGATTCCTATGAAAC pLKO_005 1865 CDS 100% 10.800 7.560 N RPH3A n/a
9 TRCN0000220583 GTGTAGTATGTGAGGACTGTA pLKO.1 650 CDS 100% 4.950 3.465 N RPH3A n/a
10 TRCN0000166364 CACACACACACACACACACAA pLKO.1 2767 3UTR 100% 4.950 2.475 Y KAAG1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001347955.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07812 pDONR223 100% 90.7% 90.8% None 0_1ins189;1074C>T;1263T>C n/a
2 ccsbBroad304_07812 pLX_304 0% 90.7% 90.8% V5 0_1ins189;1074C>T;1263T>C n/a
3 TRCN0000477369 CAACTCGTGAATAAGATCATACCT pLX_317 16.1% 90.7% 90.8% V5 0_1ins189;1074C>T;1263T>C n/a
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