Construct: ORF TRCN0000477369
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007662.1_s317c1
- Derived from:
- ccsbBroadEn_07812
- DNA Barcode:
- CAACTCGTGAATAAGATCATACCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPH3A (22895)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477369
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 22895 | RPH3A | rabphilin 3A | NM_014954.4 | 99.9% | 100% | 1263C>T;1452T>C |
| 2 | human | 22895 | RPH3A | rabphilin 3A | NM_001143854.2 | 99.3% | 99.2% | 71_82del;1275C>T;1464T>C |
| 3 | human | 22895 | RPH3A | rabphilin 3A | NM_001347952.2 | 99.3% | 99.2% | 71_82del;1275C>T;1464T>C |
| 4 | human | 22895 | RPH3A | rabphilin 3A | NM_001347953.1 | 99.3% | 99.2% | 71_82del;1275C>T;1464T>C |
| 5 | human | 22895 | RPH3A | rabphilin 3A | NM_001347954.2 | 99.3% | 99.2% | 71_82del;1275C>T;1464T>C |
| 6 | human | 22895 | RPH3A | rabphilin 3A | NM_001347955.2 | 90.7% | 90.8% | 0_1ins189;1074C>T;1263T>C |
| 7 | human | 22895 | RPH3A | rabphilin 3A | XM_017019034.2 | 50% | 50.1% | 0_1ins1032;231C>T;420T>C |
| 8 | human | 22895 | RPH3A | rabphilin 3A | NR_145126.2 | 44% | (many diffs) | |
| 9 | human | 22895 | RPH3A | rabphilin 3A | NR_145127.2 | 43.4% | (many diffs) | |
| 10 | mouse | 19894 | Rph3a | rabphilin 3A | XM_011248188.2 | 81.2% | 86.5% | (many diffs) |
| 11 | mouse | 19894 | Rph3a | rabphilin 3A | NM_001302344.1 | 81.1% | 86.2% | (many diffs) |
| 12 | mouse | 19894 | Rph3a | rabphilin 3A | NM_001302345.1 | 81.1% | 86.2% | (many diffs) |
| 13 | mouse | 19894 | Rph3a | rabphilin 3A | NM_011286.3 | 81.1% | 86.2% | (many diffs) |
| 14 | mouse | 19894 | Rph3a | rabphilin 3A | XM_011248185.2 | 80.8% | 85.9% | (many diffs) |
| 15 | mouse | 19894 | Rph3a | rabphilin 3A | XM_011248186.2 | 80.8% | 85.9% | (many diffs) |
| 16 | mouse | 19894 | Rph3a | rabphilin 3A | XM_011248187.2 | 80.8% | 85.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2136
- ORF length:
- 2070
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac tgacaccgtg ttcagcaaca gttctaaccg ttggatgtac cccagtgacc 121 ggccccttca atcaaagctc caggcaggct ggtccgtcca ccccggtggt cagcctgaca 181 ggcagaggaa gcaggaagag ctgactgatg aggagaaaga aatcatcaac agggtgattg 241 ctcgagctga gaaaatggaa gagatggagc aggagcgaat cggacgcctg gtggaccgcc 301 tagaaaacat gaggaagaac gtggctggag atggggtgaa ccgctgcata ctgtgtggag 361 aacagctggg gatgctgggc tctgcctgtg tagtatgtga ggactgtaag aagaacgtct 421 gcaccaagtg cggagtggag accaacaacc gcctgcattc tgtgtggctc tgcaaaatct 481 gcattgagca gagggaggtg tggaagcgtt ctggagcgtg gttcttcaaa ggcttcccca 541 aacaggtcct cccacagcct atgcctataa agaagaccaa gccccagcag cctgtcagtg 601 agcctgctgc ccctgaacag cctgctcctg agcccaagca ccctgcccgg gctccagctc 661 gaggtgacag tgaagatagg aggggcccgg gtcagaagac aggccctgac ccagcctctg 721 ctcccgggcg aggaaactat gggcctcccg tgcgcagggc ctccgaggca cgaatgagct 781 catctagccg agattcagag agctgggacc acagtggggg tgctggagac tccagccgga 841 gcccagcagg tttgagacgg gccaactcag tccaggcctc cagacctgcc ccaggctcgg 901 tgcagagccc agcgccacct cagcctgggc agccagggac cccaggagga agcagaccgg 961 gtcctgggcc agcaggacgc tttccagatc agaagccaga ggtggctccg agcgaccctg 1021 ggaccactgc cccaccccga gaggagagaa cagggggagt cgggggctac ccagcagttg 1081 gagccagaga ggaccgaatg agccacccct ccggacccta ttcccaagca tctgcagctg 1141 ccccccagcc tgctgcagcc cgccagccac cacccccaga ggaggaggaa gaggaagcca 1201 acagctacga ttcggatgaa gcaaccaccc tgggtgccct ggaattcagc cttctctacg 1261 accaggacaa cagctccctg cagtgcacca tcattaaggc caagggcctg aagcccatgg 1321 attcaaatgg cttggctgat ccctacgtta agctgcacct cctgccggga gccagcaagt 1381 ccaacaagct tcgtacaaaa actctgcgga atacccggaa ccccatctgg aatgagaccc 1441 tcgtgtatca cggcatcacc gatgaggaca tgcaaaggaa gaccctcagg atctccgtct 1501 gtgatgagga caaattcggc cacaatgaat ttattggtga gaccagattc tccctcaaga 1561 aactgaagcc caaccagagg aagaatttca acatctgcct ggagcgagtg attcctatga 1621 aacgtgctgg gaccaccggg tcagcccgag gcatggccct ttatgaggaa gagcaggtgg 1681 agcgtgttgg tgacatcgag gagcgtggca agatcctggt ctccctcatg tacagcacac 1741 agcagggagg cctcattgtg ggcatcatac gctgcgtgca cctggctgcc atggacgcta 1801 atggctacTC AGACCCATTC GTCAAGCTCT GGCTGAAACC GGACATGGGA AAGAAGGCCA 1861 AACACAAGAC TCAAATTAAA AAGAAAACCT TGAATCCCGA ATTCAATGAG GAGTTTTTCT 1921 ATGACATCAA ACACAGTGAC CTGGCAAAGA AGTCACTGGA CATTTCAGTC TGGGACTATG 1981 ACATCGGCAA GTCCAATGAT TACATCGGAG GCTGCCAGCT GGGGATCTCT GCCAAGGGAG 2041 AGCGCTTAAA ACACTGGTAC GAGTGTCTGA AAAATAAAGA CAAGAAGATA GAGCGCTGGC 2101 ACCAGCTACA GAATGAGAAC CACGTGTCAA GTGATTACCC AACTTTCTTG TACAAAGTGG 2161 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 2221 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 2281 ACAACTCGTG AATAAGATCA TACCTACGCG TTAAGTCgac aatcaacctc tggattacaa 2341 aatttgtgaa agatt