Construct: ORF TRCN0000477369

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007662.1_s317c1
Derived from:
ccsbBroadEn_07812
DNA Barcode:
CAACTCGTGAATAAGATCATACCT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RPH3A (22895)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000477369
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 22895 RPH3A rabphilin 3A NM_014954.4 99.9% 100% 1263C>T;1452T>C
2 human 22895 RPH3A rabphilin 3A NM_001143854.2 99.3% 99.2% 71_82del;1275C>T;1464T>C
3 human 22895 RPH3A rabphilin 3A NM_001347952.2 99.3% 99.2% 71_82del;1275C>T;1464T>C
4 human 22895 RPH3A rabphilin 3A NM_001347953.1 99.3% 99.2% 71_82del;1275C>T;1464T>C
5 human 22895 RPH3A rabphilin 3A NM_001347954.2 99.3% 99.2% 71_82del;1275C>T;1464T>C
6 human 22895 RPH3A rabphilin 3A NM_001347955.2 90.7% 90.8% 0_1ins189;1074C>T;1263T>C
7 human 22895 RPH3A rabphilin 3A XM_017019034.2 50% 50.1% 0_1ins1032;231C>T;420T>C
8 human 22895 RPH3A rabphilin 3A NR_145126.2 44% (many diffs)
9 human 22895 RPH3A rabphilin 3A NR_145127.2 43.4% (many diffs)
10 mouse 19894 Rph3a rabphilin 3A XM_011248188.2 81.2% 86.5% (many diffs)
11 mouse 19894 Rph3a rabphilin 3A NM_001302344.1 81.1% 86.2% (many diffs)
12 mouse 19894 Rph3a rabphilin 3A NM_001302345.1 81.1% 86.2% (many diffs)
13 mouse 19894 Rph3a rabphilin 3A NM_011286.3 81.1% 86.2% (many diffs)
14 mouse 19894 Rph3a rabphilin 3A XM_011248185.2 80.8% 85.9% (many diffs)
15 mouse 19894 Rph3a rabphilin 3A XM_011248186.2 80.8% 85.9% (many diffs)
16 mouse 19894 Rph3a rabphilin 3A XM_011248187.2 80.8% 85.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2136
ORF length:
2070
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgac tgacaccgtg ttcagcaaca gttctaaccg ttggatgtac cccagtgacc
121ggccccttca atcaaagctc caggcaggct ggtccgtcca ccccggtggt cagcctgaca
181ggcagaggaa gcaggaagag ctgactgatg aggagaaaga aatcatcaac agggtgattg
241ctcgagctga gaaaatggaa gagatggagc aggagcgaat cggacgcctg gtggaccgcc
301tagaaaacat gaggaagaac gtggctggag atggggtgaa ccgctgcata ctgtgtggag
361aacagctggg gatgctgggc tctgcctgtg tagtatgtga ggactgtaag aagaacgtct
421gcaccaagtg cggagtggag accaacaacc gcctgcattc tgtgtggctc tgcaaaatct
481gcattgagca gagggaggtg tggaagcgtt ctggagcgtg gttcttcaaa ggcttcccca
541aacaggtcct cccacagcct atgcctataa agaagaccaa gccccagcag cctgtcagtg
601agcctgctgc ccctgaacag cctgctcctg agcccaagca ccctgcccgg gctccagctc
661gaggtgacag tgaagatagg aggggcccgg gtcagaagac aggccctgac ccagcctctg
721ctcccgggcg aggaaactat gggcctcccg tgcgcagggc ctccgaggca cgaatgagct
781catctagccg agattcagag agctgggacc acagtggggg tgctggagac tccagccgga
841gcccagcagg tttgagacgg gccaactcag tccaggcctc cagacctgcc ccaggctcgg
901tgcagagccc agcgccacct cagcctgggc agccagggac cccaggagga agcagaccgg
961gtcctgggcc agcaggacgc tttccagatc agaagccaga ggtggctccg agcgaccctg
1021ggaccactgc cccaccccga gaggagagaa cagggggagt cgggggctac ccagcagttg
1081gagccagaga ggaccgaatg agccacccct ccggacccta ttcccaagca tctgcagctg
1141ccccccagcc tgctgcagcc cgccagccac cacccccaga ggaggaggaa gaggaagcca
1201acagctacga ttcggatgaa gcaaccaccc tgggtgccct ggaattcagc cttctctacg
1261accaggacaa cagctccctg cagtgcacca tcattaaggc caagggcctg aagcccatgg
1321attcaaatgg cttggctgat ccctacgtta agctgcacct cctgccggga gccagcaagt
1381ccaacaagct tcgtacaaaa actctgcgga atacccggaa ccccatctgg aatgagaccc
1441tcgtgtatca cggcatcacc gatgaggaca tgcaaaggaa gaccctcagg atctccgtct
1501gtgatgagga caaattcggc cacaatgaat ttattggtga gaccagattc tccctcaaga
1561aactgaagcc caaccagagg aagaatttca acatctgcct ggagcgagtg attcctatga
1621aacgtgctgg gaccaccggg tcagcccgag gcatggccct ttatgaggaa gagcaggtgg
1681agcgtgttgg tgacatcgag gagcgtggca agatcctggt ctccctcatg tacagcacac
1741agcagggagg cctcattgtg ggcatcatac gctgcgtgca cctggctgcc atggacgcta
1801atggctacTC AGACCCATTC GTCAAGCTCT GGCTGAAACC GGACATGGGA AAGAAGGCCA
1861AACACAAGAC TCAAATTAAA AAGAAAACCT TGAATCCCGA ATTCAATGAG GAGTTTTTCT
1921ATGACATCAA ACACAGTGAC CTGGCAAAGA AGTCACTGGA CATTTCAGTC TGGGACTATG
1981ACATCGGCAA GTCCAATGAT TACATCGGAG GCTGCCAGCT GGGGATCTCT GCCAAGGGAG
2041AGCGCTTAAA ACACTGGTAC GAGTGTCTGA AAAATAAAGA CAAGAAGATA GAGCGCTGGC
2101ACCAGCTACA GAATGAGAAC CACGTGTCAA GTGATTACCC AACTTTCTTG TACAAAGTGG
2161TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA
2221CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG
2281ACAACTCGTG AATAAGATCA TACCTACGCG TTAAGTCgac aatcaacctc tggattacaa
2341aatttgtgaa agatt

Download FASTA (ORF) (Full)