Transcript: Human NM_001349113.2

Homo sapiens transmembrane and coiled-coil domains 4 (TMCO4), transcript variant 3, mRNA.

Source:
NCBI, updated 2019-06-02
Taxon:
Homo sapiens (human)
Gene:
TMCO4 (255104)
Length:
3070
CDS:
339..2243

Additional Resources:

NCBI RefSeq record:
NM_001349113.2
NBCI Gene record:
TMCO4 (255104)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001349113.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000412290 ATCTCCCTGTCCCAGTTATTT pLKO_005 480 CDS 100% 15.000 10.500 N TMCO4 n/a
2 TRCN0000430085 CGGAGGAAATGGAAGCGTTAT pLKO_005 888 CDS 100% 10.800 7.560 N TMCO4 n/a
3 TRCN0000127824 GCATCCCGAAAGAAGAAAGAA pLKO.1 864 CDS 100% 5.625 3.938 N TMCO4 n/a
4 TRCN0000128110 CCTGACAGGATACAAGATGAA pLKO.1 1088 CDS 100% 4.950 3.465 N TMCO4 n/a
5 TRCN0000129357 CTCCTTCTGCACAGAGTTCAT pLKO.1 518 CDS 100% 4.950 3.465 N TMCO4 n/a
6 TRCN0000129375 GAGTCAACCAAGCACAGGTTA pLKO.1 2500 3UTR 100% 4.950 3.465 N TMCO4 n/a
7 TRCN0000130795 GCCATTGAAGAGTTCACGTTT pLKO.1 1122 CDS 100% 4.950 3.465 N TMCO4 n/a
8 TRCN0000129707 CCTAAATGGAAGGAATTGGAA pLKO.1 2355 3UTR 100% 3.000 2.100 N TMCO4 n/a
9 TRCN0000129432 GCCAGAGTCATCTACTTCTGT pLKO.1 1557 CDS 100% 3.000 2.100 N TMCO4 n/a
10 TRCN0000130796 GCATGGTGAATACATGCTGAA pLKO.1 2906 3UTR 100% 0.405 0.284 N TMCO4 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001349113.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_09906 pDONR223 100% 99.8% 99.6% None 1433_1434delGTinsAC;1717T>N n/a
2 ccsbBroad304_09906 pLX_304 0% 99.8% 99.6% V5 1433_1434delGTinsAC;1717T>N n/a
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