Construct: ORF ccsbBroad304_09906

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008595.1_s304c1, BRDN0000404892
Derived from:
ccsbBroadEn_09906
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TMCO4 (255104)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_09906
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 255104 TMCO4 transmembrane and coiled-co... NM_001349112.2 99.8% 99.6% 1433_1434delGTinsAC;1717T>N
2 human 255104 TMCO4 transmembrane and coiled-co... NM_001349113.2 99.8% 99.6% 1433_1434delGTinsAC;1717T>N
3 human 255104 TMCO4 transmembrane and coiled-co... NM_001349114.2 99.8% 99.6% 1433_1434delGTinsAC;1717T>N
4 human 255104 TMCO4 transmembrane and coiled-co... NM_181719.6 99.8% 99.6% 1433_1434delGTinsAC;1717T>N
5 human 255104 TMCO4 transmembrane and coiled-co... XM_005245822.4 99.8% 99.6% 1433_1434delGTinsAC;1717T>N
6 human 255104 TMCO4 transmembrane and coiled-co... XM_011541185.3 99.8% 99.6% 1433_1434delGTinsAC;1717T>N
7 human 255104 TMCO4 transmembrane and coiled-co... XM_011541186.2 99.8% 99.6% 1433_1434delGTinsAC;1717T>N
8 human 255104 TMCO4 transmembrane and coiled-co... XM_005245827.3 93.5% 93.3% 757_758ins120;1313_1314delGTinsAC;1597T>N
9 human 255104 TMCO4 transmembrane and coiled-co... XM_017000913.2 93.5% 93.3% 757_758ins120;1313_1314delGTinsAC;1597T>N
10 human 255104 TMCO4 transmembrane and coiled-co... XM_017000917.1 91.3% 91.1% 1_177del;1610_1611delGTinsAC;1894T>N
11 human 255104 TMCO4 transmembrane and coiled-co... XM_017000916.1 90.6% 90.5% 1_192del;1625_1626delGTinsAC;1909T>N
12 human 255104 TMCO4 transmembrane and coiled-co... XM_011541179.2 86.2% 86.1% 1_300del;1733_1734delGTinsAC;2017T>N
13 human 255104 TMCO4 transmembrane and coiled-co... XM_011541180.2 86.2% 86.1% 1_300del;1733_1734delGTinsAC;2017T>N
14 human 255104 TMCO4 transmembrane and coiled-co... XM_011541181.1 86.2% 86.1% 1_300del;1733_1734delGTinsAC;2017T>N
15 human 255104 TMCO4 transmembrane and coiled-co... XM_017000914.1 86.2% 86.1% 1_300del;1733_1734delGTinsAC;2017T>N
16 human 255104 TMCO4 transmembrane and coiled-co... XM_017000915.1 86.2% 86.1% 1_300del;1733_1734delGTinsAC;2017T>N
17 human 255104 TMCO4 transmembrane and coiled-co... XM_017000922.1 82.6% 78.8% (many diffs)
18 human 255104 TMCO4 transmembrane and coiled-co... NM_001349115.1 47.9% 47% (many diffs)
19 mouse 77056 Tmco4 transmembrane and coiled-co... XM_017320451.2 55.4% 57.5% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1968
ORF length:
1902
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc catgtggaac aggccatgcc agaggctgcc tcagcagcct ctggtagctg
121agcccactgc agagggggag ccacacctgc ccacgggccg ggagctgact gaggccaacc
181gcttcgccta tgctgccctc tgtggcatct ccctgtccca gttatttcct gaacccgaac
241acagctcctt ctgcacagag ttcatggcag gcctggtgca gtggctggag ttgtctgaag
301ctgtcttgcc aaccatgact gcttttgcga gcggcctggg aggtgaagga gcagatgtgt
361ttgttcaaat tttactgaag gaccccatct tgaaggacga cccgacggtg atcactcagg
421accttctgag cttctcactc aaggatgggc actatgacgc ccgggccaga gtcctcgttt
481gccacatgac ctccctgctc caagtgccct tggaggagct ggatgtcctt gaagagatgt
541tcctggagag cctgaaggaa atcaaagaag aggaatctga aatggccgag gcatcccgaa
601agaagaaaga aaaccggagg aaatggaagc gttatctcct gataggcctg gcgactgtcg
661gaggcggaac ggtgatcggt gtgactggag gtctagctgc accccttgtt gccgctggag
721cagcgacgat tattggcagc gccggggcag cggctctggg ctcagcagcc ggcatagcca
781tcatgacctc gctgtttggt gcagctggag ctggcctgac aggatacaag atgaagaagc
841gagtgggagc cattgaagag ttcacgtttc tgcctctgac ggagggcagg cagctgcaca
901tcaccatcgc cgtcacgggg tggctcgctt ctggcaaata ccgcaccttc agtgccccgt
961gggctgccct ggcccacagc cgtgagcagt actgcctggc ctgggaagcc aagtacctga
1021tggagctcgg caatgccctg gagaccatcc tcagtggtct cgccaacatg gtggcccagg
1081aggccctaaa gtacacagtg ttgtctggca ttgtggctgc cctgacctgg ccagcctcac
1141tcctcagtgt cgccaatgtc atcgacaacc cctggggggt gtgtctccat cgatcagcag
1201aggttggcaa gcacctggcc cacatcctgc tctcccggca gcaggggcga cgacctgtca
1261ccttgattgg cttcagcctg ggagccagag tcatctactt ctgtctgcag gagatggctc
1321aagagaaaga ttgccaagga atcatcgagg acgtcatcct gctgggtgcg cctgtggagg
1381gagaagccaa gcattgggag cctttccgga aggtggtgtc cgggaggatc atcaacggct
1441actgcagggg agactggctg ctgagtttcg tgtaccgcac atcctcggtg cagctccacg
1501tcgccggcct acagcccgtg ctgctgcagg acaggagggt ggagaacgtg gacctgacct
1561ctgtggtcag cggccacctg gactatgcca agcagatgga tgccatcctg aaggccgtgg
1621gcatccgcac caagccaggc tgggacgaga aggggctctt gctggcccca ggctgcctgc
1681cctccgagga gcctcgccag gcagcagctg ccgcctcatc aggcgagacc ccccaccagg
1741ttgggcaaac ccagggtccc atatccggag acacctccaa antggccatg tccacagacc
1801ccagccaagc ccaggtgcca gtagggctgg accagtctga aggggcctcc cttcctgctg
1861ctgccagccc tgaaaggccc cccatctgca gccatggcat ggaccccaac ccactgggct
1921gccccgattg tgcctgcaag acccagggcc ccagcacggg gctggactgc ccaactttct
1981tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt
2041aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta

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