Construct: ORF ccsbBroad304_09906
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008595.1_s304c1, BRDN0000404892
- Derived from:
- ccsbBroadEn_09906
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMCO4 (255104)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_09906
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 255104 | TMCO4 | transmembrane and coiled-co... | NM_001349112.2 | 99.8% | 99.6% | 1433_1434delGTinsAC;1717T>N |
| 2 | human | 255104 | TMCO4 | transmembrane and coiled-co... | NM_001349113.2 | 99.8% | 99.6% | 1433_1434delGTinsAC;1717T>N |
| 3 | human | 255104 | TMCO4 | transmembrane and coiled-co... | NM_001349114.2 | 99.8% | 99.6% | 1433_1434delGTinsAC;1717T>N |
| 4 | human | 255104 | TMCO4 | transmembrane and coiled-co... | NM_181719.6 | 99.8% | 99.6% | 1433_1434delGTinsAC;1717T>N |
| 5 | human | 255104 | TMCO4 | transmembrane and coiled-co... | XM_005245822.4 | 99.8% | 99.6% | 1433_1434delGTinsAC;1717T>N |
| 6 | human | 255104 | TMCO4 | transmembrane and coiled-co... | XM_011541185.3 | 99.8% | 99.6% | 1433_1434delGTinsAC;1717T>N |
| 7 | human | 255104 | TMCO4 | transmembrane and coiled-co... | XM_011541186.2 | 99.8% | 99.6% | 1433_1434delGTinsAC;1717T>N |
| 8 | human | 255104 | TMCO4 | transmembrane and coiled-co... | XM_005245827.3 | 93.5% | 93.3% | 757_758ins120;1313_1314delGTinsAC;1597T>N |
| 9 | human | 255104 | TMCO4 | transmembrane and coiled-co... | XM_017000913.2 | 93.5% | 93.3% | 757_758ins120;1313_1314delGTinsAC;1597T>N |
| 10 | human | 255104 | TMCO4 | transmembrane and coiled-co... | XM_017000917.1 | 91.3% | 91.1% | 1_177del;1610_1611delGTinsAC;1894T>N |
| 11 | human | 255104 | TMCO4 | transmembrane and coiled-co... | XM_017000916.1 | 90.6% | 90.5% | 1_192del;1625_1626delGTinsAC;1909T>N |
| 12 | human | 255104 | TMCO4 | transmembrane and coiled-co... | XM_011541179.2 | 86.2% | 86.1% | 1_300del;1733_1734delGTinsAC;2017T>N |
| 13 | human | 255104 | TMCO4 | transmembrane and coiled-co... | XM_011541180.2 | 86.2% | 86.1% | 1_300del;1733_1734delGTinsAC;2017T>N |
| 14 | human | 255104 | TMCO4 | transmembrane and coiled-co... | XM_011541181.1 | 86.2% | 86.1% | 1_300del;1733_1734delGTinsAC;2017T>N |
| 15 | human | 255104 | TMCO4 | transmembrane and coiled-co... | XM_017000914.1 | 86.2% | 86.1% | 1_300del;1733_1734delGTinsAC;2017T>N |
| 16 | human | 255104 | TMCO4 | transmembrane and coiled-co... | XM_017000915.1 | 86.2% | 86.1% | 1_300del;1733_1734delGTinsAC;2017T>N |
| 17 | human | 255104 | TMCO4 | transmembrane and coiled-co... | XM_017000922.1 | 82.6% | 78.8% | (many diffs) |
| 18 | human | 255104 | TMCO4 | transmembrane and coiled-co... | NM_001349115.1 | 47.9% | 47% | (many diffs) |
| 19 | mouse | 77056 | Tmco4 | transmembrane and coiled-co... | XM_017320451.2 | 55.4% | 57.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1968
- ORF length:
- 1902
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc catgtggaac aggccatgcc agaggctgcc tcagcagcct ctggtagctg 121 agcccactgc agagggggag ccacacctgc ccacgggccg ggagctgact gaggccaacc 181 gcttcgccta tgctgccctc tgtggcatct ccctgtccca gttatttcct gaacccgaac 241 acagctcctt ctgcacagag ttcatggcag gcctggtgca gtggctggag ttgtctgaag 301 ctgtcttgcc aaccatgact gcttttgcga gcggcctggg aggtgaagga gcagatgtgt 361 ttgttcaaat tttactgaag gaccccatct tgaaggacga cccgacggtg atcactcagg 421 accttctgag cttctcactc aaggatgggc actatgacgc ccgggccaga gtcctcgttt 481 gccacatgac ctccctgctc caagtgccct tggaggagct ggatgtcctt gaagagatgt 541 tcctggagag cctgaaggaa atcaaagaag aggaatctga aatggccgag gcatcccgaa 601 agaagaaaga aaaccggagg aaatggaagc gttatctcct gataggcctg gcgactgtcg 661 gaggcggaac ggtgatcggt gtgactggag gtctagctgc accccttgtt gccgctggag 721 cagcgacgat tattggcagc gccggggcag cggctctggg ctcagcagcc ggcatagcca 781 tcatgacctc gctgtttggt gcagctggag ctggcctgac aggatacaag atgaagaagc 841 gagtgggagc cattgaagag ttcacgtttc tgcctctgac ggagggcagg cagctgcaca 901 tcaccatcgc cgtcacgggg tggctcgctt ctggcaaata ccgcaccttc agtgccccgt 961 gggctgccct ggcccacagc cgtgagcagt actgcctggc ctgggaagcc aagtacctga 1021 tggagctcgg caatgccctg gagaccatcc tcagtggtct cgccaacatg gtggcccagg 1081 aggccctaaa gtacacagtg ttgtctggca ttgtggctgc cctgacctgg ccagcctcac 1141 tcctcagtgt cgccaatgtc atcgacaacc cctggggggt gtgtctccat cgatcagcag 1201 aggttggcaa gcacctggcc cacatcctgc tctcccggca gcaggggcga cgacctgtca 1261 ccttgattgg cttcagcctg ggagccagag tcatctactt ctgtctgcag gagatggctc 1321 aagagaaaga ttgccaagga atcatcgagg acgtcatcct gctgggtgcg cctgtggagg 1381 gagaagccaa gcattgggag cctttccgga aggtggtgtc cgggaggatc atcaacggct 1441 actgcagggg agactggctg ctgagtttcg tgtaccgcac atcctcggtg cagctccacg 1501 tcgccggcct acagcccgtg ctgctgcagg acaggagggt ggagaacgtg gacctgacct 1561 ctgtggtcag cggccacctg gactatgcca agcagatgga tgccatcctg aaggccgtgg 1621 gcatccgcac caagccaggc tgggacgaga aggggctctt gctggcccca ggctgcctgc 1681 cctccgagga gcctcgccag gcagcagctg ccgcctcatc aggcgagacc ccccaccagg 1741 ttgggcaaac ccagggtccc atatccggag acacctccaa antggccatg tccacagacc 1801 ccagccaagc ccaggtgcca gtagggctgg accagtctga aggggcctcc cttcctgctg 1861 ctgccagccc tgaaaggccc cccatctgca gccatggcat ggaccccaac ccactgggct 1921 gccccgattg tgcctgcaag acccagggcc ccagcacggg gctggactgc ccaactttct 1981 tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt 2041 aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta