Construct: ORF TRCN0000467583
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004439.1_s317c1
- Derived from:
- ccsbBroadEn_12300
- DNA Barcode:
- TTTAGCTTGTCCCACATATTATTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KYAT3 (56267)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467583
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56267 | KYAT3 | kynurenine aminotransferase 3 | NM_001349449.1 | 100% | 100% | |
| 2 | human | 56267 | KYAT3 | kynurenine aminotransferase 3 | NM_001349450.1 | 100% | 100% | |
| 3 | human | 56267 | KYAT3 | kynurenine aminotransferase 3 | NM_001008662.2 | 69% | 69% | 1_390del |
| 4 | human | 56267 | KYAT3 | kynurenine aminotransferase 3 | NM_001349447.1 | 69% | 69% | 1_390del |
| 5 | human | 56267 | KYAT3 | kynurenine aminotransferase 3 | NM_001008661.3 | 63.8% | 63.8% | 1_492del |
| 6 | human | 56267 | KYAT3 | kynurenine aminotransferase 3 | NM_001349448.1 | 63.8% | 63.8% | 1_492del |
| 7 | human | 56267 | KYAT3 | kynurenine aminotransferase 3 | NR_146183.1 | 48% | 1_657del;1528_1809del | |
| 8 | human | 56267 | KYAT3 | kynurenine aminotransferase 3 | NR_146185.1 | 40.1% | 1_714del;886_887ins121;1464_1745del | |
| 9 | human | 56267 | KYAT3 | kynurenine aminotransferase 3 | NR_146184.1 | 34.8% | 1_869del;1041_1042ins121;1619_2031del | |
| 10 | mouse | 229905 | Kyat3 | kynurenine aminotransferase 3 | NM_173763.4 | 58.8% | 59.2% | (many diffs) |
| 11 | mouse | 229905 | Kyat3 | kynurenine aminotransferase 3 | XM_006501431.3 | 58.8% | 59.2% | (many diffs) |
| 12 | mouse | 229905 | Kyat3 | kynurenine aminotransferase 3 | XM_011240114.2 | 58.8% | 59.2% | (many diffs) |
| 13 | mouse | 229905 | Kyat3 | kynurenine aminotransferase 3 | NM_001293560.1 | 54.3% | 54.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 936
- ORF length:
- 870
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt gagaatggct ggagcaacac ctgtttttat tcccctgaga tctaaacctg 121 tttatggaaa aagatggtct agttctgact ggacattaga tcctcaagaa ctggaaagta 181 aatttaattc caaaaccaaa gctattatac taaatactcc acataaccca cttggcaagg 241 tgtataacag agaggaactg caagtaattg ctgacctttg catcaaatat gacacactct 301 gcatcagcga tgaggtttat gaatggcttg tatattctgg aaataagcac ttaaaaatag 361 ctacttttcc aggtatgtgg gagagaacaa taacaatagg aagtgctgga aagactttca 421 gtgtaactgg ctggaagctt ggctggtcca ttggtccaaa tcatttgata aaacatttac 481 agacagttca acaaaacacg atttatactt gtgcaactcc tttacaggaa gccttggctc 541 aagctttctg gattGACATC AAGCGCATGG ATGACCCAGA ATGTTACTTT AATTCTTTGC 601 CAAAAGAGTT AGAAGTAAAA AGAGATCGGA TGGTACGTTT ACTTGAAAGT GTTGGCCTAA 661 AACCCATAGT TCCTGATGGA GGATACTTCA TCATCGCTGA TGTGTCTTTG CTAGATCCAG 721 ACCTCTCTGA TATGAAGAAT AATGAGCCTT ATGACTATAA GTTTGTGAAA TGGATGACTA 781 AACATAAGAA ACTATCAGCC ATCCCCGTTT CAGCATTCTG TAACTCAGAG ACTAAATCAC 841 AGTTTGAGAA GTTTGTGCGT TTTTGCTTCA TTAAAAAAGA CAGCACACTG GATGCTGCTG 901 AAGAAATCAT CAAGGCATGG AGTGTACAGA AGTCTTGCCC AACTTTCTTG TACAAAGTGG 961 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1021 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1081 ATTTAGCTTG TCCCACATAT TATTAACGCG TTAAGTCgac aatcaacctc tggattacaa 1141 aatttgtgaa agatt