Transcript: Human NM_001349522.1

Homo sapiens PX domain containing serine/threonine kinase like (PXK), transcript variant 41, mRNA.

Source:
NCBI, updated 2019-09-26
Taxon:
Homo sapiens (human)
Gene:
PXK (54899)
Length:
2934
CDS:
100..1545

Additional Resources:

NCBI RefSeq record:
NM_001349522.1
NBCI Gene record:
PXK (54899)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001145501 GGAACGGCTAGTGTTAAGCT pXPR_003 GGG 436 30% 4 0.8577 PXK PXK 78017
2 BRDN0001146866 TTATGGACGACCGCCAGACT pXPR_003 CGG 955 66% 9 0.6286 PXK PXK 78018
3 BRDN0001487114 TCTTCCGATCAGAACCAAAG pXPR_003 TGG 333 23% 3 0.1478 PXK PXK 78019
4 BRDN0001147508 CTCCAATGTGATGCTCGATG pXPR_003 GGG 790 55% 8 -0.1184 PXK PXK 78020
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001349522.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000001804 TCCGCAAACTATACTGAGATT pLKO.1 373 CDS 100% 4.950 6.930 N PXK n/a
2 TRCN0000001803 CCACAGTTTAAGATCCCTACA pLKO.1 1219 CDS 100% 4.050 5.670 N PXK n/a
3 TRCN0000342399 CCACAGTTTAAGATCCCTACA pLKO_005 1219 CDS 100% 4.050 5.670 N PXK n/a
4 TRCN0000001801 GTCTAATCAAACTTCTGCCTT pLKO.1 587 CDS 100% 2.640 2.112 N PXK n/a
5 TRCN0000342361 GTCTAATCAAACTTCTGCCTT pLKO_005 587 CDS 100% 2.640 2.112 N PXK n/a
6 TRCN0000001802 ACAGCCACAAACAGTACTATT pLKO.1 1868 3UTR 100% 13.200 9.240 N PXK n/a
7 TRCN0000194925 CGTTGCTAATTAGGATGTTTA pLKO.1 665 CDS 100% 13.200 9.240 N PXK n/a
8 TRCN0000196594 GTACAGCAACTCCAATAATTC pLKO.1 1443 CDS 100% 13.200 9.240 N PXK n/a
9 TRCN0000195613 CGGCTCTTACAGATGCCATTA pLKO.1 1165 CDS 100% 10.800 7.560 N PXK n/a
10 TRCN0000001800 GCCTTCCTTCTACCGATCTTA pLKO.1 939 CDS 100% 5.625 3.938 N PXK n/a
11 TRCN0000342398 GCCTTCCTTCTACCGATCTTA pLKO_005 939 CDS 100% 5.625 3.938 N PXK n/a
12 TRCN0000197047 GCTTCCTGTTTACACTTGGAG pLKO.1 1769 3UTR 100% 2.640 1.848 N PXK n/a
13 TRCN0000199401 CAAACTACCCTCTTCCTGGGA pLKO.1 1828 3UTR 100% 0.660 0.462 N PXK n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001349522.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12106 pDONR223 100% 81% 81.1% None 101_102ins99;804C>T;1252_1443del n/a
2 ccsbBroad304_12106 pLX_304 0% 81% 81.1% V5 101_102ins99;804C>T;1252_1443del n/a
3 TRCN0000471464 CGATAACATCTCGACTATGCAATA pLX_317 35.6% 81% 81.1% V5 101_102ins99;804C>T;1252_1443del n/a
4 ccsbBroadEn_15084 pDONR223 0% 81% 81.1% None 101_102ins99;804C>T;1252_1443del n/a
5 ccsbBroad304_15084 pLX_304 0% 81% 81.1% V5 101_102ins99;804C>T;1252_1443del n/a
6 TRCN0000465367 CCCCCTCCTCTAAACAATTGGTAC pLX_317 14.5% 81% 81.1% V5 101_102ins99;804C>T;1252_1443del n/a
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