Construct: ORF ccsbBroad304_12106

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017188.1_s304c1, BRDN0000402992
Derived from:
ccsbBroadEn_12106
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PXK (54899)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_12106
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 54899 PXK PX domain containing serine... NM_001349527.1 91.2% 91.2% 903C>T;1351_1479del
2 human 54899 PXK PX domain containing serine... NM_001349519.1 91% 91% 903C>T;1351_1482del
3 human 54899 PXK PX domain containing serine... NM_001349520.1 91% 91% 903C>T;1351_1482del
4 human 54899 PXK PX domain containing serine... NM_001349508.1 87.6% 87.7% 903C>T;1351_1539del
5 human 54899 PXK PX domain containing serine... NM_001349509.1 87.6% 87.7% 903C>T;1351_1539del
6 human 54899 PXK PX domain containing serine... XM_017006678.1 87.6% 87.7% 903C>T;1351_1539del
7 human 54899 PXK PX domain containing serine... NM_001349503.1 87.4% 87.5% 903C>T;1351_1542del
8 human 54899 PXK PX domain containing serine... NM_001349504.1 87.4% 87.5% 903C>T;1351_1542del
9 human 54899 PXK PX domain containing serine... NM_001349506.1 87.4% 87.5% 903C>T;1351_1542del
10 human 54899 PXK PX domain containing serine... NM_001349507.1 87.4% 87.5% 903C>T;1351_1542del
11 human 54899 PXK PX domain containing serine... NM_001289098.1 87.3% 87.3% 903C>T;1351_1545del
12 human 54899 PXK PX domain containing serine... NM_001349488.1 87.3% 87.3% 903C>T;1351_1545del
13 human 54899 PXK PX domain containing serine... NM_001349516.1 84.5% 84.6% 150_151ins48;855C>T;1303_1491del
14 human 54899 PXK PX domain containing serine... NM_001349511.1 84.3% 84.4% 150_151ins48;855C>T;1303_1494del
15 human 54899 PXK PX domain containing serine... NM_001349512.1 84.3% 84.4% 150_151ins48;855C>T;1303_1494del
16 human 54899 PXK PX domain containing serine... NM_001349518.1 84.3% 84.4% 101_102ins51;852C>T;1300_1488del
17 human 54899 PXK PX domain containing serine... NM_001349510.1 84.2% 84.2% 150_151ins48;855C>T;1303_1497del
18 human 54899 PXK PX domain containing serine... NM_001349515.1 84.1% 84.2% 101_102ins51;852C>T;1300_1491del
19 human 54899 PXK PX domain containing serine... NM_001349517.1 84.1% 84.2% 101_102ins51;852C>T;1300_1491del
20 human 54899 PXK PX domain containing serine... XM_017006683.1 84.1% 84.2% 101_102ins51;852C>T;1300_1491del
21 human 54899 PXK PX domain containing serine... NM_001349514.1 84% 84% 101_102ins51;852C>T;1300_1494del
22 human 54899 PXK PX domain containing serine... NM_001349524.1 81.2% 81.2% 101_102ins99;804C>T;1252_1440del
23 human 54899 PXK PX domain containing serine... NM_001349522.1 81% 81.1% 101_102ins99;804C>T;1252_1443del
24 human 54899 PXK PX domain containing serine... NM_001289100.1 80.9% 80.9% 101_102ins99;804C>T;1252_1446del
25 human 54899 PXK PX domain containing serine... NM_001349490.1 80.9% 80.9% 101_102ins99;804C>T;1252_1446del
26 human 54899 PXK PX domain containing serine... NM_001349499.1 80.7% 80.7% 903C>T;1351_1671del
27 human 54899 PXK PX domain containing serine... XM_005265250.4 77.9% 77.9% 903C>T;1351_1731del
28 human 54899 PXK PX domain containing serine... NM_001349501.1 77.8% 77.9% 150_151ins48;855C>T;1303_1623del
29 human 54899 PXK PX domain containing serine... NM_017771.5 77.7% 77.8% 903C>T;1351_1734del
30 human 54899 PXK PX domain containing serine... NM_001349502.1 77.6% 77.7% 101_102ins51;852C>T;1300_1620del
31 human 54899 PXK PX domain containing serine... NM_001349493.1 77.5% 77.5% 903C>T;1351_1740del
32 human 54899 PXK PX domain containing serine... NM_001349492.1 77.3% 77.4% 903C>T;1351_1743del
33 human 54899 PXK PX domain containing serine... NM_001349495.1 75.1% 75.2% 150_151ins48;855C>T;1303_1683del
34 human 54899 PXK PX domain containing serine... NM_001349494.1 75% 75% 150_151ins48;855C>T;1303_1686del
35 human 54899 PXK PX domain containing serine... NM_001349497.1 74.9% 75% 101_102ins51;852C>T;1300_1680del
36 human 54899 PXK PX domain containing serine... NM_001349496.1 74.8% 74.9% 101_102ins51;852C>T;1300_1683del
37 human 54899 PXK PX domain containing serine... NM_001289095.1 74.5% 74.6% 101_102ins51;852C>T;1300_1689del
38 human 54899 PXK PX domain containing serine... NM_001349498.1 72.6% 72.1% (many diffs)
39 human 54899 PXK PX domain containing serine... NM_001349500.1 72.2% 72.2% 101_102ins99;804C>T;1252_1632del
40 human 54899 PXK PX domain containing serine... NM_001289096.1 72% 72.1% 101_102ins99;804C>T;1252_1635del
41 human 54899 PXK PX domain containing serine... NM_001349530.1 71.3% 71.4% 0_1ins249;654C>T;1102_1293del
42 human 54899 PXK PX domain containing serine... NM_001349531.1 71.3% 71.4% 0_1ins249;654C>T;1102_1293del
43 human 54899 PXK PX domain containing serine... NM_001349528.1 71.1% 71.2% 0_1ins249;654C>T;1102_1296del
44 human 54899 PXK PX domain containing serine... NM_001349529.1 71.1% 71.2% 0_1ins249;654C>T;1102_1296del
45 human 54899 PXK PX domain containing serine... NM_001349521.1 63.5% 63.6% 0_1ins249;654C>T;1102_1482del
46 human 54899 PXK PX domain containing serine... NM_001289099.1 63.4% 63.4% 0_1ins249;654C>T;1102_1485del
47 human 54899 PXK PX domain containing serine... NM_001349489.1 63.4% 63.4% 0_1ins249;654C>T;1102_1485del
48 human 54899 PXK PX domain containing serine... NM_001349513.1 63.1% 63.1% 0_1ins249;654C>T;1102_1494del
49 human 54899 PXK PX domain containing serine... NM_001349539.1 60.9% 61% 0_1ins411;492C>T;940_1128del
50 human 54899 PXK PX domain containing serine... NM_001349540.1 60.9% 61% 0_1ins411;492C>T;940_1128del
51 human 54899 PXK PX domain containing serine... NM_001349535.1 60.8% 60.8% 0_1ins411;492C>T;940_1131del
52 human 54899 PXK PX domain containing serine... NM_001349536.1 60.8% 60.8% 0_1ins411;492C>T;940_1131del
53 human 54899 PXK PX domain containing serine... NM_001349537.1 60.8% 60.8% 0_1ins411;492C>T;940_1131del
54 human 54899 PXK PX domain containing serine... NM_001349538.1 60.8% 60.8% 0_1ins411;492C>T;940_1131del
55 human 54899 PXK PX domain containing serine... NM_001349533.1 60.7% 60.7% 0_1ins411;492C>T;940_1134del
56 human 54899 PXK PX domain containing serine... NM_001349534.1 60.7% 60.7% 0_1ins411;492C>T;940_1134del
57 human 54899 PXK PX domain containing serine... NM_001349532.1 56.1% 56.1% 0_1ins411;492C>T;940_1260del
58 human 54899 PXK PX domain containing serine... NM_001349526.1 54.1% 54.2% 0_1ins411;492C>T;940_1320del
59 human 54899 PXK PX domain containing serine... NM_001289101.1 54% 54.1% 0_1ins411;492C>T;940_1323del
60 human 54899 PXK PX domain containing serine... NM_001349491.1 54% 54.1% 0_1ins411;492C>T;940_1323del
61 human 54899 PXK PX domain containing serine... NM_001349525.1 53.8% 53.8% 0_1ins411;492C>T;940_1332del
62 human 54899 PXK PX domain containing serine... NR_146194.1 44.3% 1_99del;1002C>T;1450_3041del
63 human 54899 PXK PX domain containing serine... NR_146193.1 36.5% (many diffs)
64 mouse 218699 Pxk PX domain containing serine... XM_017315952.1 79.8% 84% (many diffs)
65 mouse 218699 Pxk PX domain containing serine... XM_006517982.2 77.9% 83.8% (many diffs)
66 mouse 218699 Pxk PX domain containing serine... XM_006517980.2 77.8% 83.6% (many diffs)
67 mouse 218699 Pxk PX domain containing serine... XM_006517981.2 77.8% 83.6% (many diffs)
68 mouse 218699 Pxk PX domain containing serine... XM_017315951.1 77.8% 83.6% (many diffs)
69 mouse 218699 Pxk PX domain containing serine... NM_178279.2 77.6% 83.4% (many diffs)
70 mouse 218699 Pxk PX domain containing serine... XM_006517979.3 77.6% 83.4% (many diffs)
71 mouse 218699 Pxk PX domain containing serine... XM_006517978.3 74.9% 80.5% (many diffs)
72 mouse 218699 Pxk PX domain containing serine... XM_017315950.1 74.9% 80.5% (many diffs)
73 mouse 218699 Pxk PX domain containing serine... XM_017315949.1 73.2% 78.7% (many diffs)
74 mouse 218699 Pxk PX domain containing serine... XM_006517976.3 68.8% 74% (many diffs)
75 mouse 218699 Pxk PX domain containing serine... XM_006517977.3 68.8% 74% (many diffs)
76 mouse 218699 Pxk PX domain containing serine... NM_145458.3 68.7% 73.8% (many diffs)
77 mouse 218699 Pxk PX domain containing serine... XM_006517975.3 68.7% 73.8% (many diffs)
78 mouse 218699 Pxk PX domain containing serine... XM_006517984.1 55.8% 60.1% (many diffs)
79 mouse 218699 Pxk PX domain containing serine... XM_006517985.1 55.8% 60.1% (many diffs)
80 mouse 218699 Pxk PX domain containing serine... XR_874027.2 52.3% (many diffs)
81 mouse 218699 Pxk PX domain containing serine... XR_001781106.1 39.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1416
ORF length:
1350
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc cttcatggag aagccgccag ccggcaaggt gctgctggac gacacggtgc
121cgctgacagc agccatcgag gcgagccaga gcctgcagtc ccacacggaa tatattattc
181gagtgcaaag aggaatttct gtggaaaaca gctggcagat tgttagaaga tacagtgact
241ttgatttgct taacaacagc ttacagattg caggcctaag tctacctctt cctcccaaaa
301aattgattgg taacatggat cgtgaattca tagctgaaag gcagaaaggt cttcagaact
361atctcaacgt gatcacaaca aatcatatct tgtctaattg tgagctggtt aagaagtttt
421tagatccaaa caactattcc gcaaactata ctgagattgc cttgcaacag gtttccatgt
481tcttccgatc agaaccaaag tgggaggtgg tggaaccttt gaaagacata ggttggagaa
541taaggaagaa atatttcttg atgaagatta aaaatcagcc aaaggaacgg ctagtgttaa
601gctgggctga ccttggccca gacaagtatt tgtcagataa agattttcag tgtctaatca
661aacttctgcc ttcttgtttg cacccttaca tctatcgggt tacctttgcc acagctaatg
721aatcctcagc gttgctaatt aggatgttta acgaaaaggg aacattgaag gatctgatct
781acaaggcaaa accaaaagac ccatttctaa agaagtactg caaccctaag aagattcagg
841gcctggaact ccagcaaata aaaacatatg gacggcaaat attagaggta ctgaagtttc
901ttcatgacaa gggattccct tatgggcatc ttcacgcctc caatgtgatg ctcgatgggg
961acacttgtcg gctgctggac cttgagaatt ccttattggg cctgccttcc ttctaccgat
1021cttatttttc acaattcagg aaaatcaata cattggaaag tgtggatgtc cactgctttg
1081gccacttact gtatgaaatg acttatggac gaccgccaga ctcggtgcct gtggactcct
1141tccctcctgc cccgtccatg gctgtggtgg ccgtgttgga gtctacgctg tcttgtgaag
1201cctgtaaaaa tggcatgcct accatctccc ggctcttaca gatgccatta ttcagcgatg
1261ttttactaac cacttctgaa aaaccacagt ttaagatccc tacaaagtta aaagaggcat
1321tgagaattgc caaagaatgt atagagaaga gactaattga ggaacagaaa cagattcacc
1381agcatcgaag actgacaaga gctcagtccc accattgccc aactttcttg tacaaagtgg
1441ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg
1501ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta

Download FASTA (ORF) (Full)