Construct: ORF TRCN0000465367
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017188.2_s317c1
- Derived from:
- ccsbBroadEn_15084
- DNA Barcode:
- CCCCCTCCTCTAAACAATTGGTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PXK (54899)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465367
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54899 | PXK | PX domain containing serine... | NM_001349527.1 | 91.2% | 91.2% | 903C>T;1351_1479del |
| 2 | human | 54899 | PXK | PX domain containing serine... | NM_001349519.1 | 91% | 91% | 903C>T;1351_1482del |
| 3 | human | 54899 | PXK | PX domain containing serine... | NM_001349520.1 | 91% | 91% | 903C>T;1351_1482del |
| 4 | human | 54899 | PXK | PX domain containing serine... | NM_001349508.1 | 87.6% | 87.7% | 903C>T;1351_1539del |
| 5 | human | 54899 | PXK | PX domain containing serine... | NM_001349509.1 | 87.6% | 87.7% | 903C>T;1351_1539del |
| 6 | human | 54899 | PXK | PX domain containing serine... | XM_017006678.1 | 87.6% | 87.7% | 903C>T;1351_1539del |
| 7 | human | 54899 | PXK | PX domain containing serine... | NM_001349503.1 | 87.4% | 87.5% | 903C>T;1351_1542del |
| 8 | human | 54899 | PXK | PX domain containing serine... | NM_001349504.1 | 87.4% | 87.5% | 903C>T;1351_1542del |
| 9 | human | 54899 | PXK | PX domain containing serine... | NM_001349506.1 | 87.4% | 87.5% | 903C>T;1351_1542del |
| 10 | human | 54899 | PXK | PX domain containing serine... | NM_001349507.1 | 87.4% | 87.5% | 903C>T;1351_1542del |
| 11 | human | 54899 | PXK | PX domain containing serine... | NM_001289098.1 | 87.3% | 87.3% | 903C>T;1351_1545del |
| 12 | human | 54899 | PXK | PX domain containing serine... | NM_001349488.1 | 87.3% | 87.3% | 903C>T;1351_1545del |
| 13 | human | 54899 | PXK | PX domain containing serine... | NM_001349516.1 | 84.5% | 84.6% | 150_151ins48;855C>T;1303_1491del |
| 14 | human | 54899 | PXK | PX domain containing serine... | NM_001349511.1 | 84.3% | 84.4% | 150_151ins48;855C>T;1303_1494del |
| 15 | human | 54899 | PXK | PX domain containing serine... | NM_001349512.1 | 84.3% | 84.4% | 150_151ins48;855C>T;1303_1494del |
| 16 | human | 54899 | PXK | PX domain containing serine... | NM_001349518.1 | 84.3% | 84.4% | 101_102ins51;852C>T;1300_1488del |
| 17 | human | 54899 | PXK | PX domain containing serine... | NM_001349510.1 | 84.2% | 84.2% | 150_151ins48;855C>T;1303_1497del |
| 18 | human | 54899 | PXK | PX domain containing serine... | NM_001349515.1 | 84.1% | 84.2% | 101_102ins51;852C>T;1300_1491del |
| 19 | human | 54899 | PXK | PX domain containing serine... | NM_001349517.1 | 84.1% | 84.2% | 101_102ins51;852C>T;1300_1491del |
| 20 | human | 54899 | PXK | PX domain containing serine... | XM_017006683.1 | 84.1% | 84.2% | 101_102ins51;852C>T;1300_1491del |
| 21 | human | 54899 | PXK | PX domain containing serine... | NM_001349514.1 | 84% | 84% | 101_102ins51;852C>T;1300_1494del |
| 22 | human | 54899 | PXK | PX domain containing serine... | NM_001349524.1 | 81.2% | 81.2% | 101_102ins99;804C>T;1252_1440del |
| 23 | human | 54899 | PXK | PX domain containing serine... | NM_001349522.1 | 81% | 81.1% | 101_102ins99;804C>T;1252_1443del |
| 24 | human | 54899 | PXK | PX domain containing serine... | NM_001289100.1 | 80.9% | 80.9% | 101_102ins99;804C>T;1252_1446del |
| 25 | human | 54899 | PXK | PX domain containing serine... | NM_001349490.1 | 80.9% | 80.9% | 101_102ins99;804C>T;1252_1446del |
| 26 | human | 54899 | PXK | PX domain containing serine... | NM_001349499.1 | 80.7% | 80.7% | 903C>T;1351_1671del |
| 27 | human | 54899 | PXK | PX domain containing serine... | XM_005265250.4 | 77.9% | 77.9% | 903C>T;1351_1731del |
| 28 | human | 54899 | PXK | PX domain containing serine... | NM_001349501.1 | 77.8% | 77.9% | 150_151ins48;855C>T;1303_1623del |
| 29 | human | 54899 | PXK | PX domain containing serine... | NM_017771.5 | 77.7% | 77.8% | 903C>T;1351_1734del |
| 30 | human | 54899 | PXK | PX domain containing serine... | NM_001349502.1 | 77.6% | 77.7% | 101_102ins51;852C>T;1300_1620del |
| 31 | human | 54899 | PXK | PX domain containing serine... | NM_001349493.1 | 77.5% | 77.5% | 903C>T;1351_1740del |
| 32 | human | 54899 | PXK | PX domain containing serine... | NM_001349492.1 | 77.3% | 77.4% | 903C>T;1351_1743del |
| 33 | human | 54899 | PXK | PX domain containing serine... | NM_001349495.1 | 75.1% | 75.2% | 150_151ins48;855C>T;1303_1683del |
| 34 | human | 54899 | PXK | PX domain containing serine... | NM_001349494.1 | 75% | 75% | 150_151ins48;855C>T;1303_1686del |
| 35 | human | 54899 | PXK | PX domain containing serine... | NM_001349497.1 | 74.9% | 75% | 101_102ins51;852C>T;1300_1680del |
| 36 | human | 54899 | PXK | PX domain containing serine... | NM_001349496.1 | 74.8% | 74.9% | 101_102ins51;852C>T;1300_1683del |
| 37 | human | 54899 | PXK | PX domain containing serine... | NM_001289095.1 | 74.5% | 74.6% | 101_102ins51;852C>T;1300_1689del |
| 38 | human | 54899 | PXK | PX domain containing serine... | NM_001349498.1 | 72.6% | 72.1% | (many diffs) |
| 39 | human | 54899 | PXK | PX domain containing serine... | NM_001349500.1 | 72.2% | 72.2% | 101_102ins99;804C>T;1252_1632del |
| 40 | human | 54899 | PXK | PX domain containing serine... | NM_001289096.1 | 72% | 72.1% | 101_102ins99;804C>T;1252_1635del |
| 41 | human | 54899 | PXK | PX domain containing serine... | NM_001349530.1 | 71.3% | 71.4% | 0_1ins249;654C>T;1102_1293del |
| 42 | human | 54899 | PXK | PX domain containing serine... | NM_001349531.1 | 71.3% | 71.4% | 0_1ins249;654C>T;1102_1293del |
| 43 | human | 54899 | PXK | PX domain containing serine... | NM_001349528.1 | 71.1% | 71.2% | 0_1ins249;654C>T;1102_1296del |
| 44 | human | 54899 | PXK | PX domain containing serine... | NM_001349529.1 | 71.1% | 71.2% | 0_1ins249;654C>T;1102_1296del |
| 45 | human | 54899 | PXK | PX domain containing serine... | NM_001349521.1 | 63.5% | 63.6% | 0_1ins249;654C>T;1102_1482del |
| 46 | human | 54899 | PXK | PX domain containing serine... | NM_001289099.1 | 63.4% | 63.4% | 0_1ins249;654C>T;1102_1485del |
| 47 | human | 54899 | PXK | PX domain containing serine... | NM_001349489.1 | 63.4% | 63.4% | 0_1ins249;654C>T;1102_1485del |
| 48 | human | 54899 | PXK | PX domain containing serine... | NM_001349513.1 | 63.1% | 63.1% | 0_1ins249;654C>T;1102_1494del |
| 49 | human | 54899 | PXK | PX domain containing serine... | NM_001349539.1 | 60.9% | 61% | 0_1ins411;492C>T;940_1128del |
| 50 | human | 54899 | PXK | PX domain containing serine... | NM_001349540.1 | 60.9% | 61% | 0_1ins411;492C>T;940_1128del |
| 51 | human | 54899 | PXK | PX domain containing serine... | NM_001349535.1 | 60.8% | 60.8% | 0_1ins411;492C>T;940_1131del |
| 52 | human | 54899 | PXK | PX domain containing serine... | NM_001349536.1 | 60.8% | 60.8% | 0_1ins411;492C>T;940_1131del |
| 53 | human | 54899 | PXK | PX domain containing serine... | NM_001349537.1 | 60.8% | 60.8% | 0_1ins411;492C>T;940_1131del |
| 54 | human | 54899 | PXK | PX domain containing serine... | NM_001349538.1 | 60.8% | 60.8% | 0_1ins411;492C>T;940_1131del |
| 55 | human | 54899 | PXK | PX domain containing serine... | NM_001349533.1 | 60.7% | 60.7% | 0_1ins411;492C>T;940_1134del |
| 56 | human | 54899 | PXK | PX domain containing serine... | NM_001349534.1 | 60.7% | 60.7% | 0_1ins411;492C>T;940_1134del |
| 57 | human | 54899 | PXK | PX domain containing serine... | NM_001349532.1 | 56.1% | 56.1% | 0_1ins411;492C>T;940_1260del |
| 58 | human | 54899 | PXK | PX domain containing serine... | NM_001349526.1 | 54.1% | 54.2% | 0_1ins411;492C>T;940_1320del |
| 59 | human | 54899 | PXK | PX domain containing serine... | NM_001289101.1 | 54% | 54.1% | 0_1ins411;492C>T;940_1323del |
| 60 | human | 54899 | PXK | PX domain containing serine... | NM_001349491.1 | 54% | 54.1% | 0_1ins411;492C>T;940_1323del |
| 61 | human | 54899 | PXK | PX domain containing serine... | NM_001349525.1 | 53.8% | 53.8% | 0_1ins411;492C>T;940_1332del |
| 62 | human | 54899 | PXK | PX domain containing serine... | NR_146194.1 | 44.3% | 1_99del;1002C>T;1450_3041del | |
| 63 | human | 54899 | PXK | PX domain containing serine... | NR_146193.1 | 36.5% | (many diffs) | |
| 64 | mouse | 218699 | Pxk | PX domain containing serine... | XM_017315952.1 | 79.8% | 84% | (many diffs) |
| 65 | mouse | 218699 | Pxk | PX domain containing serine... | XM_006517982.2 | 77.9% | 83.8% | (many diffs) |
| 66 | mouse | 218699 | Pxk | PX domain containing serine... | XM_006517980.2 | 77.8% | 83.6% | (many diffs) |
| 67 | mouse | 218699 | Pxk | PX domain containing serine... | XM_006517981.2 | 77.8% | 83.6% | (many diffs) |
| 68 | mouse | 218699 | Pxk | PX domain containing serine... | XM_017315951.1 | 77.8% | 83.6% | (many diffs) |
| 69 | mouse | 218699 | Pxk | PX domain containing serine... | NM_178279.2 | 77.6% | 83.4% | (many diffs) |
| 70 | mouse | 218699 | Pxk | PX domain containing serine... | XM_006517979.3 | 77.6% | 83.4% | (many diffs) |
| 71 | mouse | 218699 | Pxk | PX domain containing serine... | XM_006517978.3 | 74.9% | 80.5% | (many diffs) |
| 72 | mouse | 218699 | Pxk | PX domain containing serine... | XM_017315950.1 | 74.9% | 80.5% | (many diffs) |
| 73 | mouse | 218699 | Pxk | PX domain containing serine... | XM_017315949.1 | 73.2% | 78.7% | (many diffs) |
| 74 | mouse | 218699 | Pxk | PX domain containing serine... | XM_006517976.3 | 68.8% | 74% | (many diffs) |
| 75 | mouse | 218699 | Pxk | PX domain containing serine... | XM_006517977.3 | 68.8% | 74% | (many diffs) |
| 76 | mouse | 218699 | Pxk | PX domain containing serine... | NM_145458.3 | 68.7% | 73.8% | (many diffs) |
| 77 | mouse | 218699 | Pxk | PX domain containing serine... | XM_006517975.3 | 68.7% | 73.8% | (many diffs) |
| 78 | mouse | 218699 | Pxk | PX domain containing serine... | XM_006517984.1 | 55.8% | 60.1% | (many diffs) |
| 79 | mouse | 218699 | Pxk | PX domain containing serine... | XM_006517985.1 | 55.8% | 60.1% | (many diffs) |
| 80 | mouse | 218699 | Pxk | PX domain containing serine... | XR_874027.2 | 52.3% | (many diffs) | |
| 81 | mouse | 218699 | Pxk | PX domain containing serine... | XR_001781106.1 | 39.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1416
- ORF length:
- 1350
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cttcatggag aagccgccag ccggcaaggt gctgctggac gacacggtgc 121 cgctgacagc agccatcgag gcgagccaga gcctgcagtc ccacacggaa tatattattc 181 gagtgcaaag aggaatttct gtggaaaaca gctggcagat tgttagaaga tacagtgact 241 ttgatttgct taacaacagc ttacagattg caggcctaag tctacctctt cctcccaaaa 301 aattgattgg taacatggat cgtgaattca tagctgaaag gcagaaaggt cttcagaact 361 atctcaacgt gatcacaaca aatcatatct tgtctaattg tgagctggtt aagaagtttt 421 tagatccaaa caactattcc gcaaactata ctgagattgc cttgcaacag gtttccatgt 481 tcttccgatc agaaccaaag tgggaggtgg tggaaccttt gaaagacata ggttggagaa 541 taaggaagaa atatttcttg atgaagatta aaaatcagcc aaaggaacgg ctagtgttaa 601 gctgggctga ccttggccca gacaagtatt tgtcagataa agattttcag tgtctaatca 661 aacttctgcc ttcttgtttg cacccttaca tctatcgggt tacctttgcc acagctaatg 721 aatcctcagc gttgctaatt aggatgttta acgaaaaggg aacattgaag gatctgatct 781 acaaggcaaa accaaaagac ccatttctaa agaagtactg caaccctaag aagattcagg 841 gcctggaact ccagcaaata aaaacatatg gacggcaaat attagaggta ctgaagtttc 901 ttcatgacaa gggattccct tatgggcatc ttcacgcctc caatgtgatg ctcgatgggg 961 acacttgtcg gctgctggac cttgagaatt ccttattggg cctgccttcc ttctaccgat 1021 cttatttttc acaattcagg aaaatcaata cattggaaag tgtggatgtc cactgctttg 1081 gccacttact gtatgaaatg acttatggac gaccgccaga ctcggtgcct gtggactcct 1141 tccctcctgc cccgtccatg gctgtggtgg ccgtgttgga gtctacgctg tcttgtgaag 1201 cctgtaaaaa tggcatgcct accatctCCC GGCTCTTACA GATGCCATTA TTCAGCGATG 1261 TTTTACTAAC CACTTCTGAA AAACCACAGT TTAAGATCCC TACAAAGTTA AAAGAGGCAT 1321 TGAGAATTGC CAAAGAATGT ATAGAGAAGA GACTAATTGA GGAACAGAAA CAGATTCACC 1381 AGCATCGAAG ACTGACAAGA GCTCAGTCCC ACCATTGCCC AACTTTCTTG TACAAAGTGG 1441 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1501 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1561 ACCCCCTCCT CTAAACAATT GGTACACGCG TTAAGTCgac aatcaacctc tggattacaa 1621 aatttgtgaa agatt