Transcript: Human NM_001350334.2

Homo sapiens cilia and flagella associated protein 298 (CFAP298), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-08-09
Taxon:
Homo sapiens (human)
Gene:
CFAP298 (56683)
Length:
3448
CDS:
364..939

Additional Resources:

NCBI RefSeq record:
NM_001350334.2
NBCI Gene record:
CFAP298 (56683)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001350334.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000423650 TCCAGCCCGAGAGCCTATTAT pLKO_005 747 CDS 100% 15.000 21.000 N CFAP298 n/a
2 TRCN0000425636 GCTGATGCTGTACTATCACAG pLKO_005 789 CDS 100% 4.050 2.835 N CFAP298 n/a
3 TRCN0000138091 GAGGAGCTCAAGAGATTGGAA pLKO.1 817 CDS 100% 3.000 2.100 N CFAP298 n/a
4 TRCN0000419522 GATGATGATGCCTATTTAAAC pLKO_005 844 CDS 100% 13.200 7.920 N CFAP298 n/a
5 TRCN0000138718 GACGAAGGAATGGGCAAGAAA pLKO.1 424 CDS 100% 5.625 3.375 N CFAP298 n/a
6 TRCN0000137849 GCAGGGCTCAACGTCATTAAA pLKO.1 601 CDS 100% 15.000 7.500 Y CFAP298 n/a
7 TRCN0000434680 ATTGAAATTGAAGGATGAATG pLKO_005 350 5UTR 100% 10.800 5.400 Y CFAP298 n/a
8 TRCN0000423548 TATCGCCAAGATTCAGCAAAG pLKO_005 714 CDS 100% 6.000 3.000 Y CFAP298 n/a
9 TRCN0000135104 CTGACCGATGATCAGATTGAA pLKO.1 327 5UTR 100% 5.625 2.813 Y CFAP298 n/a
10 TRCN0000222574 CGCCTGTAATCCCAGCACTTT pLKO.1 1225 3UTR 100% 4.950 2.475 Y ERAP2 n/a
11 TRCN0000429717 AGGAAGACTTGTCGGGAACAC pLKO_005 578 CDS 100% 4.050 2.025 Y CFAP298 n/a
12 TRCN0000138318 CAAGGAGCTGAGAAGAACGAA pLKO.1 651 CDS 100% 3.000 1.500 Y CFAP298 n/a
13 TRCN0000137892 GATGGTGAAAGATGCCTTGGA pLKO.1 477 CDS 100% 2.640 1.320 Y CFAP298 n/a
14 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 1226 3UTR 100% 13.200 6.600 Y LIAS n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001350334.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03741 pDONR223 100% 65.8% 59.5% None 0_1ins229;75_76ins68 n/a
2 ccsbBroad304_03741 pLX_304 0% 65.8% 59.5% V5 0_1ins229;75_76ins68 n/a
3 TRCN0000474470 TGCGATTAAGAACCGAAAGTTTCA pLX_317 37.6% 65.8% 59.5% V5 0_1ins229;75_76ins68 n/a
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