Transcript: Human NM_001354713.2

Homo sapiens thyroid hormone receptor beta (THRB), transcript variant 10, mRNA.

Source:
NCBI, updated 2019-09-30
Taxon:
Homo sapiens (human)
Gene:
THRB (7068)
Length:
7395
CDS:
279..1664

Additional Resources:

NCBI RefSeq record:
NM_001354713.2
NBCI Gene record:
THRB (7068)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001354713.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000020347 CCACTTGGACTAGCTCAATAT pLKO.1 460 CDS 100% 13.200 18.480 N THRB n/a
2 TRCN0000330530 GACAAGCACCAATAGTCAATG pLKO_005 1030 CDS 100% 10.800 15.120 N THRB n/a
3 TRCN0000330472 CACTTGGACTAGCTCAATATT pLKO_005 461 CDS 100% 15.000 10.500 N THRB n/a
4 TRCN0000020345 CCATCCTATTCCTGTAAATAT pLKO.1 699 CDS 100% 15.000 10.500 N THRB n/a
5 TRCN0000353680 GTGCCAGGAATGTCGCTTTAA pLKO_005 758 CDS 100% 13.200 9.240 N THRB n/a
6 TRCN0000330529 GTACTTGAAACTATTGCATTT pLKO_005 1831 3UTR 100% 10.800 7.560 N THRB n/a
7 TRCN0000020344 GCCTGTGTTGAGAGAATAGAA pLKO.1 1437 CDS 100% 5.625 3.938 N THRB n/a
8 TRCN0000330528 GCCTGTGTTGAGAGAATAGAA pLKO_005 1437 CDS 100% 5.625 3.938 N THRB n/a
9 TRCN0000222373 GCCTTTGAACACTATATCAAT pLKO.1 1482 CDS 100% 5.625 3.938 N Thrb n/a
10 TRCN0000020348 CCCTTTGTTCTTGGAAGTGTT pLKO.1 1634 CDS 100% 4.950 3.465 N THRB n/a
11 TRCN0000020346 GCAACAGATTTGGTGCTGGAT pLKO.1 801 CDS 100% 2.640 1.848 N THRB n/a
12 TRCN0000222374 GCATCTATGTTGGCATGGCAA pLKO.1 784 CDS 100% 2.640 1.848 N Thrb n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001354713.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01669 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_01669 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000470229 CTGACCGCTGTTCATAGGTGCCCC pLX_317 28.8% 100% 100% V5 n/a
4 TRCN0000488447 TCATTGCCTCAAGCTTGCCTCAAC pLX_317 19.8% 100% 100% V5 (not translated due to prior stop codon) n/a
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