Transcript: Mouse NM_001360831.1

Mus musculus prothymosin alpha (Ptma), transcript variant 3, mRNA.

Source:
NCBI, updated 2019-06-23
Taxon:
Mus musculus (mouse)
Gene:
Ptma (19231)
Length:
1310
CDS:
350..610

Additional Resources:

NCBI RefSeq record:
NM_001360831.1
NBCI Gene record:
Ptma (19231)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001360831.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000429553 TATTCCGAGCATTCCAGTAAC pLKO_005 1078 3UTR 100% 10.800 6.480 N PTMA n/a
2 TRCN0000125363 GAGGCAGAGAATGGAAGAGAT pLKO.1 425 CDS 100% 4.950 2.970 N Ptma n/a
3 TRCN0000417585 ACTTCCCGTCTCAGAATTTAA pLKO_005 659 3UTR 100% 15.000 7.500 Y Ptma n/a
4 TRCN0000125361 CAAGGACTTGAAGGAGAAGAA pLKO.1 391 CDS 100% 4.950 2.475 Y Ptma n/a
5 TRCN0000125360 GCTGACAATGAGGTAGATGAA pLKO.1 494 CDS 100% 4.950 2.475 Y Ptma n/a
6 TRCN0000125359 CTGTACTATAAGTAGTTGGTT pLKO.1 1117 3UTR 100% 3.000 1.500 Y Ptma n/a
7 TRCN0000134223 GCTGTACTATAAGTAGTTGGT pLKO.1 1116 3UTR 100% 2.640 1.320 Y PTMA n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001360831.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15553 pDONR223 0% 71.4% 73.8% None (many diffs) n/a
2 ccsbBroad304_15553 pLX_304 0% 71.4% 73.8% V5 (many diffs) n/a
3 TRCN0000478994 AAGACCGGGAGATGACTCTCGTTT pLX_317 100% 71.4% 73.8% V5 (many diffs) n/a
4 ccsbBroadEn_01339 pDONR223 100% 71.4% 74.7% None (many diffs) n/a
5 ccsbBroad304_01339 pLX_304 0% 71.4% 74.7% V5 (many diffs) n/a
6 TRCN0000475496 AATGTAGACTACCTTGACCGGGAC pLX_317 64.5% 71.4% 74.7% V5 (many diffs) n/a
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