Construct: ORF TRCN0000478994
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014485.1_s317c1
- Derived from:
- ccsbBroadEn_15553
- DNA Barcode:
- AAGACCGGGAGATGACTCTCGTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PTMA (5757)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478994
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5757 | PTMA | prothymosin alpha | NM_002823.4 | 99.3% | 99% | 219A>G;224A>G |
| 2 | human | 5757 | PTMA | prothymosin alpha | NM_001099285.1 | 98.4% | 98.1% | 118_120delGAG;222A>G;227A>G |
| 3 | human | 5757 | PTMA | prothymosin alpha | XM_011511521.1 | 39.6% | 36.7% | (many diffs) |
| 4 | human | 5757 | PTMA | prothymosin alpha | XM_011511519.1 | 39.5% | 34.5% | (many diffs) |
| 5 | human | 5757 | PTMA | prothymosin alpha | XM_011511520.1 | 39.5% | 36.6% | (many diffs) |
| 6 | human | 5757 | PTMA | prothymosin alpha | XM_011511518.1 | 39.4% | 34.4% | (many diffs) |
| 7 | human | 5757 | PTMA | prothymosin alpha | XM_011511522.2 | 26.9% | 8.9% | (many diffs) |
| 8 | human | 441454 | PTMAP11 | prothymosin alpha pseudogen... | NR_028350.1 | 22.2% | (many diffs) | |
| 9 | mouse | 19231 | Ptma | prothymosin alpha | NM_008972.3 | 91.5% | 94.5% | (many diffs) |
| 10 | mouse | 19231 | Ptma | prothymosin alpha | NM_001360830.1 | 74.1% | 58.8% | (many diffs) |
| 11 | mouse | 19231 | Ptma | prothymosin alpha | NM_001360831.1 | 71.4% | 73.8% | (many diffs) |
| 12 | mouse | 19231 | Ptma | prothymosin alpha | NR_153834.1 | 21.4% | (many diffs) | |
| 13 | mouse | 19231 | Ptma | prothymosin alpha | NR_153833.1 | 15.4% | (many diffs) | |
| 14 | mouse | 623796 | Gm12504 | predicted pseudogene 12504;... | NR_040414.1 | 11.9% | (many diffs) | |
| 15 | mouse | 108168281 | LOC108168281 | uncharacterized LOC108168281 | XR_001781889.1 | 9.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 396
- ORF length:
- 330
- Sequence:
-
1 tcTTCCATTT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCATGTC AGACGCAGCC GTAGACACCA GCTCCGAAAT CACCACCAAG GACTTAAAGG 121 AGAAGAAGGA AGTTGTGGAA GAGGCAGAAA ATGGAAGAGA CGCCCCTGCT AACGGGAATG 181 CTAATGAGGA AAATGGGGAG CAGGAGGCTG ACAATGAGGT AGACGAAGAA GAGGAAGAAG 241 GTGGGGAGGA AGAGGAGGAG GAAGAAGAAG GTGATGGTGA GGAGGAGGGT GGAGATGAAG 301 ATGAGGAAGC TGAGTCAGCT ACGGGCAAGC GGGCAGCTGA AGATGATGAG GATGACGATG 361 TCGATACCAA GAAGCAGAAG ACCGACGAGG ATGACTACCC AACTTTCTTG TACAAAGTGG 421 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 481 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 541 AAAGACCGGG AGATGACTCT CGTTTACGCG TTAAGTCgac aatcaacctc tggattacaa 601 aatttgtgaa agatt