Construct: ORF TRCN0000475496

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016648.1_s317c1
Derived from:
ccsbBroadEn_01339
DNA Barcode:
AATGTAGACTACCTTGACCGGGAC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PTMA (5757)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000475496
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5757 PTMA prothymosin alpha NM_002823.4 100% 100%
2 human 5757 PTMA prothymosin alpha NM_001099285.1 99% 99% 118_120delGAG
3 human 5757 PTMA prothymosin alpha XM_011511521.1 39.9% 37.1% (many diffs)
4 human 5757 PTMA prothymosin alpha XM_011511519.1 39.8% 34.9% (many diffs)
5 human 5757 PTMA prothymosin alpha XM_011511520.1 39.7% 37% (many diffs)
6 human 5757 PTMA prothymosin alpha XM_011511518.1 39.6% 34.7% (many diffs)
7 human 5757 PTMA prothymosin alpha XM_011511522.2 27.1% 8.9% (many diffs)
8 human 441454 PTMAP11 prothymosin alpha pseudogen... NR_028350.1 22.3% (many diffs)
9 mouse 19231 Ptma prothymosin alpha NM_008972.3 91.5% 95.4% (many diffs)
10 mouse 19231 Ptma prothymosin alpha NM_001360830.1 74.1% 58.8% (many diffs)
11 mouse 19231 Ptma prothymosin alpha NM_001360831.1 71.4% 74.7% (many diffs)
12 mouse 19231 Ptma prothymosin alpha NR_153834.1 21.5% (many diffs)
13 mouse 19231 Ptma prothymosin alpha NR_153833.1 15.4% (many diffs)
14 mouse 623796 Gm12504 predicted pseudogene 12504;... NR_040414.1 12% (many diffs)
15 mouse 108168281 LOC108168281 uncharacterized LOC108168281 XR_001781889.1 9.3% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
396
ORF length:
330
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgtc agacgcagcc gtagacacca gctccgaaat caccaccaag gacttaaagg
121agaagaagga agttgtggaa gaggcagaaa atggaagaga cgcccctgct aacgggaatg
181CTAATGAGGA AAATGGGGAG CAGGAGGCTG ACAATGAGGT AGACGAAGAA GAGGAAGAAG
241GTGGGGAGGA AGAGGAGGAG GAAGAAGAAG GTGATGGTGA GGAAGAGGAT GGAGATGAAG
301ATGAGGAAGC TGAGTCAGCT ACGGGCAAGC GGGCAGCTGA AGATGATGAG GATGACGATG
361TCGATACCAA GAAGCAGAAG ACCGACGAGG ATGACTACCC AACTTTCTTG TACAAAGTGG
421TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA
481CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG
541AAATGTAGAC TACCTTGACC GGGACACGCG TTAAGTCgac aatcaacctc tggattacaa
601aatttgtgaa agatt

Download FASTA (ORF) (Full)