Transcript: Human NM_001363365.2

Homo sapiens ubiquitin associated and SH3 domain containing B (UBASH3B), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-06-01
Taxon:
Homo sapiens (human)
Gene:
UBASH3B (84959)
Length:
6869
CDS:
438..2282

Additional Resources:

NCBI RefSeq record:
NM_001363365.2
NBCI Gene record:
UBASH3B (84959)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001363365.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000073151 GCGTTCAGACTGCACACAATA pLKO.1 1753 CDS 100% 13.200 18.480 N UBASH3B n/a
2 TRCN0000073148 CCGGCTTATTTGAGTGGACAA pLKO.1 1822 CDS 100% 4.050 5.670 N UBASH3B n/a
3 TRCN0000073150 GCCTGAGAATTACATTACCAA pLKO.1 1259 CDS 100% 3.000 4.200 N UBASH3B n/a
4 TRCN0000423202 GAATGCAGCACCTGGATATTT pLKO_005 1287 CDS 100% 15.000 10.500 N UBASH3B n/a
5 TRCN0000427144 ATTAGAGAGCAATACCATTAT pLKO_005 1700 CDS 100% 13.200 9.240 N UBASH3B n/a
6 TRCN0000427599 CATATTTCCTTTCACACTTAA pLKO_005 2395 3UTR 100% 13.200 9.240 N UBASH3B n/a
7 TRCN0000426360 CTCCAAGGACTTCGTACAAAT pLKO_005 2114 CDS 100% 13.200 9.240 N UBASH3B n/a
8 TRCN0000427820 GAGTGGTGGTTTCCGAGATTA pLKO_005 1616 CDS 100% 13.200 9.240 N UBASH3B n/a
9 TRCN0000073149 CCTCATAAGAAGCAGCTACAT pLKO.1 948 CDS 100% 4.950 3.465 N UBASH3B n/a
10 TRCN0000073152 CCTGTGAAGAATTAGGAGAAA pLKO.1 2167 CDS 100% 4.950 2.970 N UBASH3B n/a
11 TRCN0000140238 GTCTCCCAAAGTGCTAGGATT pLKO.1 3974 3UTR 100% 4.950 2.475 Y PDZD7 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001363365.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04453 pDONR223 100% 94.2% 89.3% None 0_1ins109;105_108delTGGT n/a
2 ccsbBroad304_04453 pLX_304 0% 94.2% 89.3% V5 0_1ins109;105_108delTGGT n/a
3 TRCN0000467371 CGTGAAGCTTCAAACGTCTTAAGC pLX_317 19.5% 94.2% 89.3% V5 0_1ins109;105_108delTGGT n/a
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