Transcript: Human NM_001363943.2

Homo sapiens angiomotin like 2 (AMOTL2), transcript variant 4, mRNA.

Source:
NCBI, updated 2019-08-09
Taxon:
Homo sapiens (human)
Gene:
AMOTL2 (51421)
Length:
4852
CDS:
155..2494

Additional Resources:

NCBI RefSeq record:
NM_001363943.2
NBCI Gene record:
AMOTL2 (51421)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001363943.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000138854 GAAGACCATGCGGAACAAGAT pLKO.1 1273 CDS 100% 4.950 3.960 N AMOTL2 n/a
2 TRCN0000134692 GAACAAGATGGACAGTGAAAT pLKO.1 1285 CDS 100% 13.200 9.240 N AMOTL2 n/a
3 TRCN0000134693 GAGAGATTGGAATCTGCAAAT pLKO.1 1340 CDS 100% 10.800 7.560 N AMOTL2 n/a
4 TRCN0000138672 GTCAGAACAACTGCGAGAGAA pLKO.1 1798 CDS 100% 4.950 3.465 N AMOTL2 n/a
5 TRCN0000135770 CAGTACCCTCATGTTGTACTA pLKO.1 788 CDS 100% 0.495 0.347 N AMOTL2 n/a
6 TRCN0000138653 CCGGGATCTTAGAGAGAGATT pLKO.1 1327 CDS 100% 4.950 2.970 N AMOTL2 n/a
7 TRCN0000138406 CCCAGAAGACTCTGCTTCTTA pLKO.1 2691 3UTR 100% 0.563 0.338 N AMOTL2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001363943.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11985 pDONR223 100% 59.8% 59.8% None 1_939del n/a
2 ccsbBroad304_11985 pLX_304 0% 59.8% 59.8% V5 (not translated due to frame shift) 1_939del n/a
3 TRCN0000468344 GTATCTAGCAACGCACGCGAGGTC pLX_317 29.3% 59.8% 59.8% V5 (not translated due to prior stop codon) 1_939del n/a
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