Transcript: Human NM_001825.2

Homo sapiens creatine kinase, mitochondrial 2 (CKMT2), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-09-21
Taxon:
Homo sapiens (human)
Gene:
CKMT2 (1160)
Length:
1650
CDS:
243..1502

Additional Resources:

NCBI RefSeq record:
NM_001825.2
NBCI Gene record:
CKMT2 (1160)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001147363 AAATGAGGAGGATCACACCA pXPR_003 GGG 805 64% 8 1.2094 CKMT2 CKMT2 76595
2 BRDN0001148542 GTCATCAAACTAAGACACAA pXPR_003 CGG 392 31% 5 0.5189 CKMT2 CKMT2 76597
3 BRDN0001147460 CCCAGCACATGTTAATAAAG pXPR_003 GGG 703 56% 7 -0.0462 CKMT2 CKMT2 76596
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001825.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000219736 ATGAGCGCCTAGGATACATTT pLKO.1 1165 CDS 100% 13.200 18.480 N CKMT2 n/a
2 TRCN0000219735 ACGGATCTGGATGCATCAAAG pLKO.1 669 CDS 100% 10.800 15.120 N CKMT2 n/a
3 TRCN0000006063 CCAGGGTAATCTCAATGGAAA pLKO.1 1048 CDS 100% 4.950 6.930 N CKMT2 n/a
4 TRCN0000006062 GAAACGAGTATTTGAGCGATT pLKO.1 1082 CDS 100% 4.050 5.670 N CKMT2 n/a
5 TRCN0000196618 GAACGGTTAATCCAAGAACGA pLKO.1 1125 CDS 100% 2.640 2.112 N CKMT2 n/a
6 TRCN0000219737 AGTCATCGATGGAGTCAATTA pLKO.1 1400 CDS 100% 13.200 9.240 N CKMT2 n/a
7 TRCN0000006061 CGTCATCAAACTAAGACACAA pLKO.1 617 CDS 100% 4.950 3.465 N CKMT2 n/a
8 TRCN0000010992 GTCGCAGATGTGTACGACATT pLKO.1 1332 CDS 100% 4.950 3.465 N CKMT2 n/a
9 TRCN0000199482 GCAGAAAGTGTGTGCCGAGGT pLKO.1 344 CDS 100% 0.720 0.504 N CKMT2 n/a
10 TRCN0000196397 GACCACTTTCTGTTTGATAAG pLKO.1 912 CDS 100% 10.800 5.400 Y CKMT2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001825.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00315 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_00315 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000491337 CAGTATGCTTATTATAATTAGTCT pLX_317 26.3% 100% 100% V5 n/a
4 ccsbBroadEn_14586 pDONR223 0% 100% 100% None n/a
5 ccsbBroad304_14586 pLX_304 0% 100% 100% V5 n/a
6 TRCN0000480045 GTCAGCAACGCCTGAAGGCTCGAC pLX_317 26.3% 100% 100% V5 n/a
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