Construct: ORF TRCN0000480045

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009236.2_s317c1
Derived from:
ccsbBroadEn_14586
DNA Barcode:
GTCAGCAACGCCTGAAGGCTCGAC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CKMT2 (1160)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000480045
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1160 CKMT2 creatine kinase, mitochondr... NM_001099735.2 100% 100%
2 human 1160 CKMT2 creatine kinase, mitochondr... NM_001099736.1 100% 100%
3 human 1160 CKMT2 creatine kinase, mitochondr... NM_001825.2 100% 100%
4 human 1160 CKMT2 creatine kinase, mitochondr... XM_024454356.1 78.1% 78.1% 1_351del
5 mouse 76722 Ckmt2 creatine kinase, mitochondr... NM_198415.2 88.8% 95.9% (many diffs)
6 mouse 76722 Ckmt2 creatine kinase, mitochondr... XM_006517454.3 46.4% 49.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1323
ORF length:
1257
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc cagtatcttt tctaagttgc taactggccg caatgcttct ctgctgtttg
121ctaccatggg caccagtgtc ctgaccaccg ggtacctgct gaaccggcag aaagtgtgtg
181ccgaggtccg ggagcagcct aggctatttc ctccaagcgc agactaccca gacctgcgca
241agcacaacaa ctgcatggcc gagtgcctca cccccgccat ttatgccaag cttcgcaaca
301aggtgacacc caacggctac acgctggacc agtgcatcca gactggagtg gacaaccctg
361gccacccctt cataaagact gtgggcatgg tggctggtga cgaggagtcc tatgaggtgt
421ttgctgacct ttttgacccc gtcatcaaac taagacacaa cggctatgac cccagggtga
481tgaagcacac aacggatctg gatgcatcaa agatcaccca agggcagttc gacgagcatt
541acgtgctgtc ttctcgggtg cgcactggcc gcagcatccg tgggctgagc ctgcctccag
601cctgcacccg ggccgagcga agggaggtag agaacgtggc catcactgcc ctggagggcc
661tcaaggggga cctggctggc cgctactaca agctgtccga gatgacggag caggaccagc
721agcggctcat cgatgaccac tttctgtttg ataagccagt gtccccttta ttaacatgtg
781ctgggatggc ccgtgactgg ccagatgcca ggggaatctg gcataattat gataagacat
841ttctcatctg gataaatgag gaggatcaca ccagggtaat ctcaatggaa aaaggaggca
901atatgaaacg agtatttgag cgattctgtc gtggactaaa agaagtagaa cggttaatcc
961aagaacgagg ctgggagttc atgtggaatg agcgccTAGG ATACATTTTG ACCTGTCCTT
1021CGAACCTTGG AACAGGACTA CGAGCTGGTG TCCACGTTAG GATCCCAAAG CTCAGCAAGG
1081ACCCACGCTT TTCTAAGATC CTGGAAAACC TAAGACTCCA GAAGCGTGGC ACAGGTGGTG
1141TGGACACTGC CGCGGTCGCA GATGTGTACG ACATTTCCAA CATAGATAGA ATTGGTCGAT
1201CAGAGGTTGA GCTTGTTCAG ATAGTCATCG ATGGAGTCAA TTACCTGGTG GATTGTGAAA
1261AGAAGTTGGA GAGAGGCCAA GATATTAAGG TGCCACCCCC TCTGCCTCAG TTTGGCAAAA
1321AGTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC
1381TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT
1441GGCTTTATAT ATCTTGTGGA AAGGACGAGT CAGCAACGCC TGAAGGCTCG ACACGCGTTA
1501AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt

Download FASTA (ORF) (Full)