Construct: ORF TRCN0000480045
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009236.2_s317c1
- Derived from:
- ccsbBroadEn_14586
- DNA Barcode:
- GTCAGCAACGCCTGAAGGCTCGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CKMT2 (1160)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480045
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1160 | CKMT2 | creatine kinase, mitochondr... | NM_001099735.2 | 100% | 100% | |
| 2 | human | 1160 | CKMT2 | creatine kinase, mitochondr... | NM_001099736.1 | 100% | 100% | |
| 3 | human | 1160 | CKMT2 | creatine kinase, mitochondr... | NM_001825.2 | 100% | 100% | |
| 4 | human | 1160 | CKMT2 | creatine kinase, mitochondr... | XM_024454356.1 | 78.1% | 78.1% | 1_351del |
| 5 | mouse | 76722 | Ckmt2 | creatine kinase, mitochondr... | NM_198415.2 | 88.8% | 95.9% | (many diffs) |
| 6 | mouse | 76722 | Ckmt2 | creatine kinase, mitochondr... | XM_006517454.3 | 46.4% | 49.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1323
- ORF length:
- 1257
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cagtatcttt tctaagttgc taactggccg caatgcttct ctgctgtttg 121 ctaccatggg caccagtgtc ctgaccaccg ggtacctgct gaaccggcag aaagtgtgtg 181 ccgaggtccg ggagcagcct aggctatttc ctccaagcgc agactaccca gacctgcgca 241 agcacaacaa ctgcatggcc gagtgcctca cccccgccat ttatgccaag cttcgcaaca 301 aggtgacacc caacggctac acgctggacc agtgcatcca gactggagtg gacaaccctg 361 gccacccctt cataaagact gtgggcatgg tggctggtga cgaggagtcc tatgaggtgt 421 ttgctgacct ttttgacccc gtcatcaaac taagacacaa cggctatgac cccagggtga 481 tgaagcacac aacggatctg gatgcatcaa agatcaccca agggcagttc gacgagcatt 541 acgtgctgtc ttctcgggtg cgcactggcc gcagcatccg tgggctgagc ctgcctccag 601 cctgcacccg ggccgagcga agggaggtag agaacgtggc catcactgcc ctggagggcc 661 tcaaggggga cctggctggc cgctactaca agctgtccga gatgacggag caggaccagc 721 agcggctcat cgatgaccac tttctgtttg ataagccagt gtccccttta ttaacatgtg 781 ctgggatggc ccgtgactgg ccagatgcca ggggaatctg gcataattat gataagacat 841 ttctcatctg gataaatgag gaggatcaca ccagggtaat ctcaatggaa aaaggaggca 901 atatgaaacg agtatttgag cgattctgtc gtggactaaa agaagtagaa cggttaatcc 961 aagaacgagg ctgggagttc atgtggaatg agcgccTAGG ATACATTTTG ACCTGTCCTT 1021 CGAACCTTGG AACAGGACTA CGAGCTGGTG TCCACGTTAG GATCCCAAAG CTCAGCAAGG 1081 ACCCACGCTT TTCTAAGATC CTGGAAAACC TAAGACTCCA GAAGCGTGGC ACAGGTGGTG 1141 TGGACACTGC CGCGGTCGCA GATGTGTACG ACATTTCCAA CATAGATAGA ATTGGTCGAT 1201 CAGAGGTTGA GCTTGTTCAG ATAGTCATCG ATGGAGTCAA TTACCTGGTG GATTGTGAAA 1261 AGAAGTTGGA GAGAGGCCAA GATATTAAGG TGCCACCCCC TCTGCCTCAG TTTGGCAAAA 1321 AGTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1381 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1441 GGCTTTATAT ATCTTGTGGA AAGGACGAGT CAGCAACGCC TGAAGGCTCG ACACGCGTTA 1501 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt