Transcript: Human NM_001874.4

Homo sapiens carboxypeptidase M (CPM), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-07-06
Taxon:
Homo sapiens (human)
Gene:
CPM (1368)
Length:
6683
CDS:
76..1407

Additional Resources:

NCBI RefSeq record:
NM_001874.4
NBCI Gene record:
CPM (1368)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001874.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000050133 CCAGTATGACTTGAATCGAAA pLKO.1 519 CDS 100% 4.950 6.930 N CPM n/a
2 TRCN0000288883 CCAGTATGACTTGAATCGAAA pLKO_005 519 CDS 100% 4.950 6.930 N CPM n/a
3 TRCN0000050135 CCAACAAATATGGAGAGTATT pLKO.1 1109 CDS 100% 13.200 9.240 N CPM n/a
4 TRCN0000288950 CCAACAAATATGGAGAGTATT pLKO_005 1109 CDS 100% 13.200 9.240 N CPM n/a
5 TRCN0000050137 CCAGAACTTCAGTGCTCTTAA pLKO.1 1221 CDS 100% 13.200 9.240 N CPM n/a
6 TRCN0000288951 CCAGAACTTCAGTGCTCTTAA pLKO_005 1221 CDS 100% 13.200 9.240 N CPM n/a
7 TRCN0000050134 CCATTTGATAATGGTGTTCAA pLKO.1 670 CDS 100% 0.495 0.347 N CPM n/a
8 TRCN0000050136 CCCTGAAATCACAAATCTGAT pLKO.1 396 CDS 100% 4.950 2.970 N CPM n/a
9 TRCN0000288948 CCCTGAAATCACAAATCTGAT pLKO_005 396 CDS 100% 4.950 2.970 N CPM n/a
10 TRCN0000166201 CATGGTGAAACCCTGTCTCTA pLKO.1 1911 3UTR 100% 4.950 2.475 Y ORAI2 n/a
11 TRCN0000136653 GCCTGACCAACATGGTGAAAT pLKO.1 1901 3UTR 100% 13.200 6.600 Y IQCC n/a
12 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 5003 3UTR 100% 13.200 6.600 Y LIAS n/a
13 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 6478 3UTR 100% 5.625 2.813 Y KLHL30 n/a
14 TRCN0000179120 CAACATGGTGAAACCCTGTTT pLKO.1 1908 3UTR 100% 4.950 2.475 Y LOC339059 n/a
15 TRCN0000138772 GCAGGAGAATCGCTTGAACTT pLKO.1 2874 3UTR 100% 4.950 2.475 Y DCAF11 n/a
16 TRCN0000140719 GATCACTTGAGGTCAGGAGTT pLKO.1 2743 3UTR 100% 4.050 2.025 Y P3H4 n/a
17 TRCN0000165299 GATCACTTGAGGTCAGGAGTT pLKO.1 2743 3UTR 100% 4.050 2.025 Y ORAI2 n/a
18 TRCN0000352971 GATCACTTGAGGTCAGGAGTT pLKO_005 2743 3UTR 100% 4.050 2.025 Y P3H4 n/a
19 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 6478 3UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001874.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06031 pDONR223 100% 99.9% 100% None 417T>C n/a
2 ccsbBroad304_06031 pLX_304 0% 99.9% 100% V5 417T>C n/a
3 TRCN0000480430 ATTCGGAGAGACCCCCTCCGAGGT pLX_317 35.3% 99.9% 100% V5 417T>C n/a
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