Construct: ORF TRCN0000470212
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017569.1_s317c1
- Derived from:
- ccsbBroadEn_00657
- DNA Barcode:
- ACCTGTCAGCCCGGTTGATGGGTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GNG4 (2786)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470212
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2786 | GNG4 | G protein subunit gamma 4 | NM_001098721.1 | 100% | 100% | |
| 2 | human | 2786 | GNG4 | G protein subunit gamma 4 | NM_001098722.2 | 100% | 100% | |
| 3 | human | 2786 | GNG4 | G protein subunit gamma 4 | NM_004485.4 | 100% | 100% | |
| 4 | human | 2786 | GNG4 | G protein subunit gamma 4 | XM_006711761.2 | 100% | 100% | |
| 5 | human | 2786 | GNG4 | G protein subunit gamma 4 | XM_011544167.2 | 100% | 100% | |
| 6 | mouse | 14706 | Gng4 | guanine nucleotide binding ... | NM_001302997.1 | 89.7% | 98.6% | (many diffs) |
| 7 | mouse | 14706 | Gng4 | guanine nucleotide binding ... | NM_010317.3 | 89.7% | 98.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 213
- ORF end:
- 438
- ORF length:
- 225
- Sequence:
-
1 TTATGCGATG GAGTTTCCCC ACACTGAGTG GGTGGAGACT GAAGTTAGGC CAGCTTGGCA 61 CTTGATGTAA TTCTCCTTGG AATTTGCCCT TTTTGAGTTT GGATCTTGGT TCATTCTCAA 121 GCCTCAGACA GTGGTTCAAA GTTTTTTTCT TCCATTTCAG GTGTCGTGAG GCTAGCATCG 181 ATTGATCAAC AAGTTTGTAC AAAAAAGTTG GCATGAAAGA GGGCATGTCT AATAACAGCA 241 CCACTAGCAT CTCCCAAGCC AGGAAAGCTG TGGAGCAGCT AAAGATGGAA GCCTGTATGG 301 ACAGGGTCAA GGTCTCCCAG GCAGCTGCGG ACCTCCTGGC CTACTGTGAA GCTCACGTGC 361 GGGAAGATCC TCTCATCATT CCAGTGCCTG CATCAGAAAA CCCCTTTCGC GAGAAGAAGT 421 TCTTTTGTAC CATTCTCTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 481 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 541 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAACCTGTC AGCCCGGTTG 601 ATGGGTAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt